Trypsin‐Immobilized Metal–Organic Framework as a Biocatalyst In Proteomics Analysis

Synthesis of patterned enzyme–metal–organic framework composites by ink-jet printing

“…For example, by incubating enzyme (or protein) molecules with pre-synthesized MOFs in aqueous solution, enzyme (or protein) molecules were adsorbed into the large pores of MOFs (Lykourinou et al 2011;Chen et al 2012;Deng et al 2012;Feng et al 2015;Li et al 2016a, b;Zhang et al 2017;Lian et al 2016). By chemical reactions between amino acids of enzyme and functional groups of pre-synthesized MOFs, enzyme molecules were attached on the surface of MOFs (Jung et al 2011;Shih et al 2012). Alternatively, another strategy is in situ one-pot synthesis of enzyme-MOF composites directly from enzyme, metal ions, and organic ligands in solution, which has been investigated by several groups (Lyu et al 2014;Shieh et al 2015;Liang et al 2015a;Lian et al 2016;Liao et al 2017).…”

Preparation of a Nanobiocatalyst by Efficiently ImmobilizingAspergillus nigerLipase onto Magnetic Metal–Biomolecule Frameworks (BioMOF)

“…The higher porosity and larger surface areas of MOFs also make the biocatalyst to react easily with its substrate. Moreover, MOFs have a large amount of functional groups, which provide a large amount binding sites to immobilize enzymes, resulting in a significant increase of the enzyme protein loading capacity . Moreover, MOF‐coated magnetic NPs allow for an easy separation of the immobilized enzyme from the reaction system and enzyme recycling with the help of external magnetic forces.…”

Fast Multipoint Immobilized MOF Bioreactor

“…The dye molecular dimension plays a role in its overall performance.Studies on metal-organic frameworks (MOFs) as solid support materials have reached the area of functionalization of biomolecules on the MOFs to serve as bioreactors or biocatalysts to initiate reactions useful in specific area of research. [1][2][3][4][5][6] Most biomolecules, such as enzymes, are macromolecules with at least one of the dimensions measuring 3 to 5 nm. [7] However, commonly used MOFs are usually microporous (< 2 nm) and only few mesoporous MOFs (!…”

“…As far as the literature is concerned, very few studies have been reported on MOF materials utilized as solid support for the immobilization of biomolecules, even less so than other MOF applications. [1][2][3][4][5][6] The unmatched molecular dimensions of the biomolecules with that of the pores of microporous MOFs restricts the entry and the eventual residence of these molecules in the MOFs, which remains a challenge. This is why most MOFs are used as adsorbents for small molecules, such as dyes [8,9] and organic compounds, [10,11] which usually conform with the pore sizes of most MOFs.…”

“…High-sequence coverage of 69 % was attained with further functionalization of MIL-88B(Cr) with amine groups (referred to in the study as MIL-88B-NH 2 (Cr)). [6] In another study, physical adsorption of trypsin onto our fabricated CYCU-4 MOF with the aid of vortex mixing was realized; the trypsin bioreactor, referred as trypsin-FITC@CYCU-4, was produced without chemical modification of the solid support thus we regarded this as a novel bioreactor. [18] To fabricate this bioreactor, trypsin adsorption onto the MOF was facilitated by fluorescein isothiocyanate (FITC) dye, a small-molecule dye compared to the pores of the MOF used.…”