Fast Multipoint Immobilized MOF Bioreactor

Abstract

An enzyme-NBD@MOF bioreactor with exemplary proteolytic performance, even after successive reuse and storage, was produced through a novel, rapid and simple multipoint immobilization technique without chemical modification of the solid support. Enzyme loading and distribution could be directly monitored from the fluorescence emission of the bioreactor. The dye molecular dimension plays a role in its overall performance.

Stable metal-organic frameworks containing single-molecule traps for enzyme encapsulation

“…In addition, cages usually have small windows, which can efficiently encapsulate enzymes, diminishing aggregation, eliminating leaching and optimizing the catalytic environment. Unfortunately, there are very few reported MOFs with enzyme compatible cages, and even fewer can survive in aqueous media 3,11,12 . Thus, MOFs with larger cages and better stability are highly desired for enzyme encapsulation.…”

Coupling two enzymes into a tandem nanoreactor utilizing a hierarchically structured MOF

“…The total enzyme encapsulation capacity was 300 wt%, which is the highest among all MOFs. 9,16,17 In the following discussion the nanoreactor generated with the →GOx → HRP order is named PCN-888-en.…”

Magnetic MOF microreactors for recyclable size-selective biocatalysis

“…There remain few reports of immobilization of active enzymes onto MOF supports, and current strategies involve the adsorption of biomolecules into mesoporous 6 and ultra-large pore frameworks, 7 covalent surface modification of MOF crystals with proteins 8 or host–guest interactions between tag-group(s) on the enzyme and MOF pores to anchor the large functional molecules to the surface. 9 These post-synthetic methods are not without their drawbacks, and typically lead to significant reductions in porosity as the large protein molecules occupy pores or block (surface) apertures to the available framework microporosity; although not necessarily a barrier to function, this could be an issue for the selectivity and/or molecular diffusion of composite biocatalytic materials. Further, adsorption directly into MOF pores necessarily restricts the size of the proteins that can enter the pores 7 which may need to undergo conformational changes 10 that could compromise biomolecule activity.…”