Trypsin and N-aminopeptidase (APN) activities in the hepatopancreas of an intertidal euryhaline crab: Biochemical characteristics and differential modulation by histamine and salinity

Michiels, María Soledad; Valle, Juana Cristina del; Mañanes, Alejandra Antonia López

doi:10.1016/j.cbpa.2016.12.003

Cited by 13 publications

(4 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The activity of APN in crustaceans is not limited to salinity adaptation. APN activity in the hepatopancreas of the euryhaline crab ( Cyrtograpsus angulatus ) was changed in response to salinity, pH, and water temperature challenges ( 35 ). This enzyme plays an important role in the final stages of protein digestion in mammals, and its activity in the intestine is considered an important indicator of protein digestive capacity ( 36 ).…”

Section: Discussionmentioning

confidence: 99%

The role of the renin-angiotensin system (RAS) in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

Farhadi

Liu

et al. 2022

Front. Endocrinol.

View full text Add to dashboard Cite

The renin-angiotensin system (RAS) is a hormonal system that plays an important role in the regulation of blood pressure and cardiovascular homeostasis in mammals. In fishes, the RAS pathway participates in osmoregulation and salinity adaptation. However, the role of the RAS pathway in invertebrates, particularly in crustaceans, remains unknown. In this study, four key genes of the RAS pathway (LV-ACE, LV-APN, LV-AT1R, and LV-RR) were cloned, characterized, and their expression levels were detected in the eyestalk, hepatopancreas, and muscle of Litopenaeus vannamei during long-term and short-term low salinity stress. The results showed that LV-ACE, LV-APN, LV-AT1R, and LV-RR encode 666, 936, 175, and 323 amino acids, respectively. Low salinity stress downregulated the expression levels of LV-ACE, LV-APN, LV-AT1R, and LV-RR in L. vannamei, indicating that the RAS pathway was suppressed under low salinity. Moreover, these genes play important roles in the regulation of drinking rate, controlling urine output, blood glucose, and blood pressure, indicating that their downregulation probably affected the homeostasis of shrimps. These findings provide novel insights into the mechanism of salinity adaptation in L. vannamei.

show abstract

Section: Discussionmentioning

confidence: 99%

The role of the renin-angiotensin system (RAS) in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

Farhadi

Liu

et al. 2022

Front. Endocrinol.

View full text Add to dashboard Cite

show abstract

“…Protein assays were conducted according to Bradford (1976) monitoring the increase in absorption at 595 nm produced by the binding of the dye Coomasie Brilliant Blue G-50 to proteins (spectrophotometer ZL5000 PLUS, Zeltec). Bovine serum albumin was used as protein concentration standard to build the corresponding standard curve and to calculate the protein concentrations of the samples (pinoni et al 2013;Michiels et al 2015aMichiels et al , 2017.…”

Section: Biochemical Assaysmentioning

confidence: 99%

“…The hepatopancreas of decapod crustaceans is a sensitive indicator organ for digestive and metabolic adjustments to distinct physiological and / or environmental conditions (Belgrad & griffen 2016;pinoni et al 2018;lópez Mañanes et al 2020). In various intertidal burrowing crabs, the modulation of specific digestive enzymes and/or energy reserves in the hepatopancreas is part of the responses involved in biochemical adaptation to key environmental challenges such as salinity and food availability (Michiels et al 2017;pinoni et al 2018;lópez Mañanes et al 2020). In intertidal crabs of the same species successfully inhabiting different habitats in a same intertidal area (e.g., mudflat or saltmarsh) of coastal lagoons, the distinct and particular environmental conditions in each habitat may impose distinct challenges leading to intraspecific differences in responses, for example, at the biochemical level (pinoni et al 2011;Mueller 2017;lópez Mañanes et al 2020).…”

Section: Introductionmentioning

confidence: 99%

The influence of habitat on metabolic and digestive parameters in an intertidal crab from a SW Atlantic coastal lagoon

2021

View full text Add to dashboard Cite

The hepatopancreas of decapod crustaceans is an organ which can act as indicator for digestive and metabolic parameters under different physiological and / or environmental conditions. However, biochemical studies on digestive and metabolic parameters of the hepatopancreas of euryhaline burrowing crabs such as Neohelice granulata from habitats with different diet compositions are still scarce. In the wild, adult males of N. granulata from Mar Chiquita Coastal Lagoon (Argentina) in mudflat habitat have diets with higher lipid and protein content than crabs from the saltmarsh, suggesting that diets could be an important factor influencing hepatopancreas activities. We tested this hypothesis here by exposing adult male crabs to a similar experimental diet and comparing hepatopancreas parameters for lipid components and protein metabolism between males from these two habitat types at different times (up to three months). At month 3, we noticed a decrease of the triglyceride concentration and lipase activity and an increase of protein concentration in crabs from the mudflat. In contrast, triglycerides and protein concentration did not change in crabs from the saltmarsh, while lipase activity decreased and levamisole insensitive AP increased at month 3. The results indicate that digestive and metabolic parameters in the hepatopancreas of crabs from habitats varying in diet content remain different, even if crabs are subsequently fed by a similar experimental diet. This suggests that specific intrinsic regulations of these hepatopancreas parameters could operate differently in each habitat and could not be changed by recent diet conditions.

show abstract

“…Free glucose was assessed from assay without α-amyloglucosidase.Triglycerides (TAG) were measured by the colorimetric method of glycerol phosphate oxidase (code 861110001, Wiener-Lab AA). An aliquot of the corresponding sample was incubated with this reactant for 5 min at 37 C(Michiels et al, 2017;Pinoni et al, 2011). The amount of released glycerol was determined by reading the absorbance at 505 nm of the coloured quinone complex.…”

mentioning

confidence: 99%

Understanding the early ontogenetic stages of Mugil liza (Mugilidae): Morphological traits and digestive/metabolic profile of pre‐juveniles after recruitment

Albanesi

González‐Castro

López-Mañanes

2020

Journal of Fish Biology

View full text Add to dashboard Cite

The family Mugilidae consists mainly of diadromous species, whose reproduction occurs in offshore waters. Pre-juveniles shift their diet in the surf zone (zooplanktophagous to iliophagous). Later, during their recruitment into estuaries, huge changes take place in their digestive system. However, digestive and metabolic characteristics and some morphological traits at recruitment are unknown for Mugilidae. We performed comparative studies on early and late pre-juveniles of Mugil liza recruited in Mar Chiquita Coastal Lagoon (37 32 0-37 45 0 S, 57 19 0-57 26 0 W, Argentina). We determined digestive enzyme activities (intestine), energy reserves (liver/muscle), total/standard length, total weight, intestinal coefficient, hepatosomatic index and retroperitoneal fat. Pre-juveniles exhibited amylase, maltase, sucrase, lipase, trypsin and aminopeptidase-N (APN) activities, which were maintained over a wide range of pH and temperature, and exhibited Michaelis-Menten kinetics. In late pre-juveniles, amylase (422 ± 131 μmol maltose min −1 mgprot −1), sucrase (86 ± 14 mg glucose min −1 mgprot −1), trypsin (84 ± 9 μmoles min −1 mgprot −1) and APN (0.58 ± 0.08 μmoles min −1 mgprot −1) activities were higher (42%, 28%, 35% and 28%, respectively) than in the early stage. Also, the intestinal coefficient was higher in late (3.04) compared to early (2.06) pre-juveniles. Moreover, the liver appeared to be a main site of glycogen and triglyceride storage in late pre-juveniles, muscle being the site of storage in early pre-juveniles, exhibiting higher glycogen, free glucose and protein concentrations (92%, 82%, 32%, respectively). The results suggest that pre-juveniles of M. liza exhibit an adequate digestive battery to perform complete hydrolysis of various dietary substrates, availability of energy reserves and morphological characteristics to support their feeding habit and growth after recruitment. Our results represent an important contribution to knowledge of the ecology and digestive physiology of pre-juveniles of Mugilidae in the wild. K E Y W O R D S digestive enzymes, energy reserves, Mar Chiquita Coastal Lagoon, Mugil liza, pre-juveniles, recruitment Mariano González-Castro and Alejandra López-Mañanes have contributed equally to this study.

show abstract

Trypsin and N-aminopeptidase (APN) activities in the hepatopancreas of an intertidal euryhaline crab: Biochemical characteristics and differential modulation by histamine and salinity

Cited by 13 publications

References 53 publications

The role of the renin-angiotensin system (RAS) in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

The role of the renin-angiotensin system (RAS) in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

The influence of habitat on metabolic and digestive parameters in an intertidal crab from a SW Atlantic coastal lagoon

Understanding the early ontogenetic stages of Mugil liza (Mugilidae): Morphological traits and digestive/metabolic profile of pre‐juveniles after recruitment

Contact Info

Product

Resources

About