The role of the renin-angiotensin system (RAS) in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

Abstract

The renin-angiotensin system (RAS) is a hormonal system that plays an important role in the regulation of blood pressure and cardiovascular homeostasis in mammals. In fishes, the RAS pathway participates in osmoregulation and salinity adaptation. However, the role of the RAS pathway in invertebrates, particularly in crustaceans, remains unknown. In this study, four key genes of the RAS pathway (LV-ACE, LV-APN, LV-AT1R, and LV-RR) were cloned, characterized, and their expression levels were detected in the eyestalk, hepatopancreas, and muscle of Litopenaeus vannamei during long-term and short-term low salinity stress. The results showed that LV-ACE, LV-APN, LV-AT1R, and LV-RR encode 666, 936, 175, and 323 amino acids, respectively. Low salinity stress downregulated the expression levels of LV-ACE, LV-APN, LV-AT1R, and LV-RR in L. vannamei, indicating that the RAS pathway was suppressed under low salinity. Moreover, these genes play important roles in the regulation of drinking rate, controlling urine output, blood glucose, and blood pressure, indicating that their downregulation probably affected the homeostasis of shrimps. These findings provide novel insights into the mechanism of salinity adaptation in L. vannamei.

Impact of water temperature on the shipping of berried female broodstock (Macrobrachium rosenbergii): A study on survival, egg retention, hatching duration, and hatching success rate

“…In the similar with P. japonicus in salinity 37 and 21 ppt. and temperatures 10-14°C and 25°C, this research indicated the higher excretion of ammonia depended on high temperature and low salinity (Farhadi et al, 2022).…”

Effects of Salinity Stress on Histological Changes, Glucose Metabolism Index and Transcriptomic Profile in Freshwater Shrimp, Macrobrachium nipponense

“…Studies have shown that the expression of anthocyanin and rhodopsin genes can help shrimp to cope with salinity stress [ 23 ]. In addition, studies have shown that the renin-angiotensin system (RAS) plays important roles in salinity adaptation in Penaeus vannamei [ 24 ]. At present, there are more and more transcriptomic studies being conducted on the response of aquatic organisms to saline environments.…”