Trypanosoma cruzi: Involvement of glycoinositolphospholipids in the attachment to the luminal midgut surface of Rhodnius prolixus

Nogueira, Nadir F. S.; Gonzalez, Marcelo S.; Gomes, José Eugênio P. Lima; Souza, Wanderley de; Garcia, Elói S.; Nohara, Lilian L.; Almeida, Igor C.; Zingales, Bianca; Colli, Walter

doi:10.1016/j.exppara.2006.12.014

Cited by 77 publications

(73 citation statements)

References 41 publications

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“…This parasite concentration is similar to that used in several other published studies of T. cruzi infection in triatomines, 9,44,46,50,[54][55][56][57][58][59] and blood meals taken by triatomines at this parasite concentration lead to infective doses that fall within the range of peak parasitemias observed in mice and guinea pigs experimentally infected with T. cruzi. 25,27,[60][61][62][63] Insect infection: insect preparation and feeding.…”

Section: Methodssupporting

confidence: 54%

Rhodnius prolixus Life History Outcomes Differ when Infected with Different Trypanosoma cruzi I Strains

Peterson

Graham

Dobson

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. The effect of a parasite on the life history of its vector is important for understanding and predicting disease transmission. Chagas disease agent Trypanosoma cruzi is a generalist parasite that is diverse across scales from its genetic diversity to the 100s of mammal and vector species it infects. Its vertebrate hosts show quite variable responses to infection, however, to date there are no studies looking at how T. cruzi variability might result in variable outcomes in its invertebrate host. Therefore, we investigated the effect of different T. cruzi I strains on Rhodnius prolixus survival and development. We found significant variation between insects infected with different strains, with some strains having no effect, as compared with uninfected insects, and others with significantly lower survival and development. We also found that different variables had varying importance between strains, with the effect of time postinfection and the blood:weight ratio of the infective meal significantly affecting the survival of insects infected with some strains, but not others. Our results suggest that T. cruzi can be pathogenic not only to its vertebrate hosts but also to its invertebrate hosts.

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Section: Methodssupporting

confidence: 54%

Rhodnius prolixus Life History Outcomes Differ when Infected with Different Trypanosoma cruzi I Strains

Peterson

Graham

Dobson

et al. 2015

The American Society of Tropical Medicine and Hygiene

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“…The parasite suspension (200 lL) was incubated with the perimicrovillar membrane of each insect group inside microtubes for 30 min at 25°C. Then the midgut preparations were spread onto glass slides and the number of attached parasites was counted under a microscope (Nogueira et al, 2007). A hundred randomly chosen epithelial cells from 10 different sites of each midgut preparation were counted.…”

Section: T Cruzi Gut Adhesionmentioning

confidence: 99%

Physalin B inhibits Trypanosoma cruzi infection in the gut of Rhodnius prolixus by affecting the immune system and microbiota

Castro

Moraes

Gonzalez

et al. 2012

Journal of Insect Physiology

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Physalin B is a natural secosteroidal, extracted from the Solanaceae plant, Physalis angulata, and it presents immune-modulator effects on the bloodsucking bug, Rhodnius prolixus. In this work, R. prolixus was treated with physalin B at a concentration of 1 mg/ml of blood meal (oral application), or 20 ng/insect (applied topically) or 57 ng/cm(2) of filter paper (contact treatment), and infected with Trypanosoma cruzi Dm28c clone (2×10(6) epimastigotes/insect). The three types of applications significantly decreased the number of T. cruzi Dm28c in the gut comparing with the non-treated infected insects (controls). All groups of infected insects treated with physalin B had higher numbers of bacterial microbiota in the gut than the non-treated controls infected with T. cruzi. We observed that the infected physalin B insects with topical and contact treatments had a lower antibacterial activity in the gut when compared with control infected insects. Furthermore, infected insects with the physalin B oral treatment produced higher levels of nitrite and nitrate in the gut than control infected insects. These results demonstrate that physalin B decreases the T. cruzi transmission by inhibiting the parasite development in the insect vector R. prolixus. Herein the importance of physalin B modulation on the immune system and microbiota population in terms of parasite development and transmission are discussed.

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“…Fourth, GIPLs are involved in parasite attachment to the midgut of the insect vector. The administration of 0.5 μM of GIPLs inhibits up to 90% parasite infection in Rhodnius prolixus [39].…”

Section: Trypanosoma Cruzimentioning

confidence: 99%

Structures of Glycolipids Found in Trypanosomatids: Contribution to Parasite Functions

Barreto‐Bergter¹

2010

TOPARAJ

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Neutral monohexosylceramides (CMHs) globosides (globotriasyl ceramides), other glycosphingolipids (GSLs) and more complex structures such as glycoinositol-phospholipids(GIPLs) and glycosyl phosphatidylinositol (GPI) anchors have been described in several members of the trypanosomatid family. These highly bioactive molecules are not only components of biological structures but also participants in host-parasite interactions such as macrophage invasion, antigenic presentation and signal transduction. Glycolipid structures have been studied using mass spectrometry (MS).This review describes a wide range of glycoconjugates with unique and complex structures that are present in several trypanosomatid species. Their structures are described in the context of their biological significance.

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Trypanosoma cruzi: Involvement of glycoinositolphospholipids in the attachment to the luminal midgut surface of Rhodnius prolixus

Cited by 77 publications

References 41 publications

Rhodnius prolixus Life History Outcomes Differ when Infected with Different Trypanosoma cruzi I Strains

Rhodnius prolixus Life History Outcomes Differ when Infected with Different Trypanosoma cruzi I Strains

Physalin B inhibits Trypanosoma cruzi infection in the gut of Rhodnius prolixus by affecting the immune system and microbiota

Structures of Glycolipids Found in Trypanosomatids: Contribution to Parasite Functions

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