Structures of Glycolipids Found in Trypanosomatids: Contribution to Parasite Functions

Barreto‐Bergter, Eliana

doi:10.2174/1874421401004010084

Cited by 11 publications

(11 citation statements)

References 144 publications

(184 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Interestingly, the glycosidic part is constituted by a sulphated disaccharide composed of hexuronic acid linked to a hexose unit. Although the hexose was not characterized, glucose or galactose is probably present as both of these sugars are known to be transferred to the ceramide moiety as the first step of the biosynthesis of complex glycosphingolipids (38–41). Glycolipids containing sulphated hexuronic acid are not common but have been identified in human dorsal root and sympathetic ganglia (42–44).…”

Section: Resultsmentioning

confidence: 99%

Structural and immunological characterization of sulphatides: relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates

Acosta

Soprano

Ferrero

et al. 2012

Parasite Immunology

View full text Add to dashboard Cite

Summary Sulphoglycosphingolipids, present on the surface of diverse cells, participate in the regulation of various cellular events. However, little is known about the structure and the role of sulphoglycosphingolipids in trypanosomatids. Herein, sulphated dihexosylceramide structures – composed mainly of sphingosine as the long chain base acylated with stearic acid – have been determined for the first time in Trypanosoma cruzi epimastigotes by UV‐MALDI‐TOF‐MS analysis. Interestingly, inhibition ELISA assays using cruzipain as antigen and polyclonal rabbit antibodies specific for cruzipain, the major cysteine proteinase of T. cruzi, or for its C‐terminal domain, have demonstrated (i) that sulphate epitopes are shared between cruzipain and sulphatides of T. cruzi, (ii) that cross‐reactivity maps to the C‐terminal domain and (iii) the existence of other antigenic determinants in the glycolipidic structures. These features provide evidence that sulphate groups are antigenic in sulphate‐containing parasite glycoconjugates. Furthermore, IgG2 antibody levels inversely correlate with disease severity in chronic Chagas disease patients, suggesting that IgG2 antibodies specific for sulphated epitopes might be associated with protective immunity and might be considered as potential surrogates of the course of chronic Chagas disease.

show abstract

Section: Resultsmentioning

confidence: 99%

Structural and immunological characterization of sulphatides: relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates

Acosta

Soprano

Ferrero

et al. 2012

Parasite Immunology

View full text Add to dashboard Cite

show abstract

“…Glycoproteins are involved in the attachment and invasion of the host cells, 4–8 while glycoinositolphospholipids help to suppress its immune system. 9,10 These actions are recognized as key factors for the pathogenesis and permanence of the parasite in the human body. 11–13 Because of this, it is believed that inhibiting Gal f formation would be a target for chemical intervention.…”

Section: ■ Introductionmentioning

confidence: 99%

Molecular Dynamics Simulations of Substrate Release from Trypanosoma cruzi UDP-Galactopyranose Mutase

Cossio-Pérez

Pierdominici‐Sottile

Sobrado

et al. 2019

J. Chem. Inf. Model.

View full text Add to dashboard Cite

The enzyme UDP-galactopyranose mutase (UGM) represents a promising drug target for the treatment of infections with Trypanosoma cruzi. We have computed the Potential of Mean Force for the release of UDP-galactopyranose from UGM, using Umbrella Sampling simulations. The simulations revealed the conformational changes that both substrate and enzyme undergo during the process. It was determined that the galactopyranose portion of the substrate is highly mobile and that the opening/closing of the active site occurs in stages. Previously uncharacterized interactions with highly conserved residues were also identified. These findings provide new pieces of information that contribute to the rational design of drugs against T. cruzi.

show abstract

“…On the other hand, the surface of the protozoan membrane is very conserved among individuals of this group, including the presence of glycosylphosphatidylinositol (GPI)-anchored glycoproteins, a covering that surrounds the cell membranes and forms the glycocalyx, a boundary between the parasite and the external environment, which also helps to form a negative net charge membrane. The glycocalyx mediates cell attachment, protects against harmful molecular and cellular agents, like AMPs, preventing their action on the membrane and/or affecting other vital functions [80,81].…”

Section: Differences Between Plasma Membranes Of Protozoan and Mammentioning

confidence: 99%

Natural Occurrence in Venomous Arthropods of Antimicrobial Peptides Active against Protozoan Parasites

Júnior

Menezes

Araújo

et al. 2019

Toxins

View full text Add to dashboard Cite

Arthropoda is a phylum of invertebrates that has undergone remarkable evolutionary radiation, with a wide range of venomous animals. Arthropod venom is a complex mixture of molecules and a source of new compounds, including antimicrobial peptides (AMPs). Most AMPs affect membrane integrity and produce lethal pores in microorganisms, including protozoan pathogens, whereas others act on internal targets or by modulation of the host immune system. Protozoan parasites cause some serious life-threatening diseases among millions of people worldwide, mostly affecting the poorest in developing tropical regions. Humans can be infected with protozoan parasites belonging to the genera Trypanosoma, Leishmania, Plasmodium, and Toxoplasma, responsible for Chagas disease, human African trypanosomiasis, leishmaniasis, malaria, and toxoplasmosis. There is not yet any cure or vaccine for these illnesses, and the current antiprotozoal chemotherapeutic compounds are inefficient and toxic and have been in clinical use for decades, which increases drug resistance. In this review, we will present an overview of AMPs, the diverse modes of action of AMPs on protozoan targets, and the prospection of novel AMPs isolated from venomous arthropods with the potential to become novel clinical agents to treat protozoan-borne diseases.

show abstract

Structures of Glycolipids Found in Trypanosomatids: Contribution to Parasite Functions

Cited by 11 publications

References 144 publications

Structural and immunological characterization of sulphatides: relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates

Structural and immunological characterization of sulphatides: relevance of sulphate moieties in Trypanosoma cruzi glycoconjugates

Molecular Dynamics Simulations of Substrate Release from Trypanosoma cruzi UDP-Galactopyranose Mutase

Natural Occurrence in Venomous Arthropods of Antimicrobial Peptides Active against Protozoan Parasites

Contact Info

Product

Resources

About