Trypanosoma cruzi infection in Triatoma sordida before and after community-wide residual insecticide spraying in the Argentinean Chaco

Macchiaverna, Natalia Paula; Gaspe, María Sol; Enriquez, Gustavo Fabián; Tomassone, Laura; Gürtler, Ricardo E.; Cardinal, Marta Victoria

doi:10.1016/j.actatropica.2014.12.010

Cited by 18 publications

(21 citation statements)

References 40 publications

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“…1998). This species shows great capacity of adaptation to peridomestic sites, especially in association with chickens (Macchiaverna et al 2015). In the current study, all specimens ( n = 203) were negative for flagellates probably because their principal food sources are avian.…”

Section: Discussionmentioning

confidence: 99%

Hosts and vectors of Trypanosoma cruzi discrete typing units in the Chagas disease endemic region of the Paraguayan Chaco

et al. 2017

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SUMMARYActive Trypanosoma cruzi transmission persists in the Gran Chaco region, which is considered hyperendemic for Chagas disease. Understanding domestic and sylvatic transmission cycles and therefore the relationship between vectors and mammalian hosts is crucial to designing and implementing improved effective control strategies. Here we describe the species of triatomine vectors and the sylvatic mammal reservoirs of T. cruzi, in different localities of the Paraguayan and Bolivian Chaco. We identify the T. cruzi genotypes discrete typing units (DTUs) and provide a map of their geographical distribution. A total of 1044 triatomines and 138 sylvatic mammals were captured. Five per cent of the triatomines were microscopically positive for T. cruzi (55 Triatoma infestans from Paraguay and one sylvatic Triatoma guasayana from Bolivia) and 17 animals (12·3%) comprising eight of 28 (28·5%) Dasypus novemcinctus, four of 27 (14·8%) Euphractus sexcinctus, three of 64 (4·7%) Chaetophractus spp. and two of 14 (14·3%) Didelphis albiventris. The most common DTU infecting domestic triatomine bugs was TcV (64%), followed by TcVI (28%), TcII (6·5%) and TcIII (1·5%). TcIII was overwhelmingly associated with armadillo species. We confirm the primary role of T. infestans in domestic transmission, armadillo species as the principal sylvatic hosts of TcIII, and consider the potential risk of TcIII as an agent of Chagas disease in the Chaco.

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Section: Discussionmentioning

confidence: 99%

Hosts and vectors of Trypanosoma cruzi discrete typing units in the Chagas disease endemic region of the Paraguayan Chaco

et al. 2017

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“…Otros autores han descrito infestación intradomiciliar de T. sordida en áreas previamente ocupadas por T. infestans, pero con un índice de infestación e infección natural inferior (17)(18)(19) al observado en este trabajo.…”

Section: Discussionunclassified

Triatoma sordida: indicadores de adaptación y transmisión de Trypanosoma cruzi en intradomicilio del Chaco Paraguayo

Sánchez

Russomando

Chena

et al. 2016

Mem. Inst. Investig. Cienc. Salud

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El gran Chaco es una eco-región que incluye Argentina, Bolivia y Paraguay; donde la trasmisión vectorial de la enfermedad de Chagas por Triatoma infestans (vector principal), constituye hasta la fecha un problema de salud pública. El Chaco paraguayo ocupa el 25% de esta región, caracterizada por una baja densidad poblacional y localidades dispersas. El objetivo de este estudio fue determinar el rol potencial de la especie Triatoma sórdida (vector secundario) en el ciclo doméstico de transmisión de Trypanosoma cruzi. Se aplicaron técnicas moleculares asociadas a indicadores entomológicos y epidemiológicosa436 ejemplares de T. sordida capturados en 147 viviendas del Chaco Paraguayo. Se detectó infestación y colonización en el intradomicilio y peridomicilio por T. sordida en 12 (8.2%) y 79 (53.7%) viviendas de las 147 evaluadas, respectivamente. Al menos un ejemplar infectado con T. cruzi fue detectado por PCR en las 12 viviendas con colonización intradomiciliar y en dos de ellas por caracterización molecular se detectó en ninfas el genotipo TC2. Adultos y ninfas en el peridomicilio de 4 viviendas dieron positivo para el genotipo TC1. Se estima un elevado riesgo de transmisión de T. cruzi intradomiciliar del 87%.Estos resultados evidencian capacidad adaptativa de esta especie en el domicilio, y un incremento de su potencial vectorial para transmitir la enfermedad de Chagas en el Chaco Paraguayo. Palabras claves: T. cruzi, genotipos, T.sordida, infestación, colonizaciónTriatoma sordida: indicators of adaptive capacity and Trypanosoma cruzi intradomicile transmission in the Paraguayan Chaco A B S T R A C TThe Gran Chaco is an eco-region that includes Argentina, Bolivia and Paraguay; where the vectorial transmission of Chagas disease by Triatoma infestans (main vector) is a public health problem up to date. The Paraguayan Chaco occupies 25% of this region, characterized by low population density and dispersed locations. The aim of this study was to determine the potential role of the specie Triatoma sordida (secondary vector) in the domestic cycle of transmission of Trypanosoma cruzi. Molecular techniques associated with entomological and epidemiological indicators were applied in 436 specimens of T. sordida captured in 147 dwellings of the Paraguayan Chaco. Infestation rate and colonization by T. Sordida was detected in the intradomicile and peridomiciliary in 12 (8.2%) and 79 (53.7%) of the 147 dwellings, respectively. In 12 houses were colonization was observed, at least one specimen was PCR-T. cruzi positive, and in two of them genotype TC2 of T. cruzi were

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“…TcI is a highly diverse DTU frequently found in opossums and rodents residing in sylvatic habitats (Cura et al, 2012;Guhl and Ramírez, 2011;Llewellyn et al, 2009). TcI infection occurred in approximately 6% of Triatoma sordida collected in (peri)domestic habitats, and bug infections were locally aggregated in partial coincidence with a hotspot of marsupials, armadillos and rodents infected with TcI or TcIII (Macchiaverna et al, 2015). TcI was isolated from T. cruzi-seropositive dogs, cats and Didelphis opossums from the disturbed area (Enriquez et al, 2014;Orozco et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

A comparative study of Trypanosoma cruzi infection in sylvatic mammals from a protected and a disturbed area in the Argentine Chaco

et al. 2016

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Understanding the complex epidemiology of Trypanosoma cruzi transmission cycles requires comparative studies in widely different environments. We assessed the occurrence of T. cruzi infection in sylvatic mammals, their infectiousness to the vector, and parasite genotypes in a protected area of the Argentine Chaco, and compared them with information obtained similarly in a nearby disturbed area. A total of 278 mammals from >23 species in the protected area were diagnosed for T. cruzi infection using xenodiagnosis, kDNA-PCR and nuclear satellite DNA-PCR (SAT) from blood samples. The relative abundance and species composition differed substantially between areas. Didelphis albiventris opossums were less abundant in the protected area; had a significantly lower body mass index, and a stage structure biased toward earlier stages. The capture of armadillos was lower in the protected area. The composite prevalence of T. cruzi infection across host species was significantly lower in the protected area (11.1%) than in the disturbed area (22.1%), and heterogeneous across species groups. The prevalence of infection in D. albiventris and Thylamys pusilla opossums was significantly lower in the protected area (nil for D. albiventris), whereas infection in sigmodontine rodents was three times higher in the protected area (17.5 versus 5.7%). Parasite isolates from the two xenodiagnosis-positive mammals (1 Dasypus novemcinctus and 1 Conepatus chinga) were typed as TcIII; both specimens were highly infectious to Triatoma infestans. Fat-tailed opossums, bats and rodents were kDNA-PCR-positive and xenodiagnosis-negative. Desmodus rotundus and Myotis bats were found infected with T. cruzi for the first time in the Gran Chaco.

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Trypanosoma cruzi infection in Triatoma sordida before and after community-wide residual insecticide spraying in the Argentinean Chaco

Cited by 18 publications

References 40 publications

Hosts and vectors of Trypanosoma cruzi discrete typing units in the Chagas disease endemic region of the Paraguayan Chaco

Hosts and vectors of Trypanosoma cruzi discrete typing units in the Chagas disease endemic region of the Paraguayan Chaco

Triatoma sordida: indicadores de adaptación y transmisión de Trypanosoma cruzi en intradomicilio del Chaco Paraguayo

A comparative study of Trypanosoma cruzi infection in sylvatic mammals from a protected and a disturbed area in the Argentine Chaco

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