2021
DOI: 10.1371/journal.pntd.0009729
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Trypanosoma cruzi infection follow-up in a sylvatic vector of Chagas disease: Comparing early and late stage nymphs

Abstract: Chagas disease is caused by Trypanosoma cruzi and transmitted by the triatomine Mepraia spinolai in the southwest of South America. Here, we examined the T. cruzi-infection dynamics of field-caught M. spinolai after laboratory feeding, with a follow-up procedure on bug populations collected in winter and spring of 2017 and 2018. Bugs were analyzed twice to evaluate T. cruzi-infection by PCR assays of urine/fecal samples, the first evaluation right after collection and the second 40 days after the first feeding… Show more

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Cited by 5 publications
(2 citation statements)
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“…Interestingly, three out of the five M. spinolai populations showing absence of molting were time-related. Specifically, C3, C4, and W4—all 2018′ populations—presented maximum rates of molting failure in a year with an unusually warm fall [ 48 ]. Therefore, our results of molting failure could be interpreted as diapause by environmental stochasticity, which seems to end after an unknown period of time after failure.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Interestingly, three out of the five M. spinolai populations showing absence of molting were time-related. Specifically, C3, C4, and W4—all 2018′ populations—presented maximum rates of molting failure in a year with an unusually warm fall [ 48 ]. Therefore, our results of molting failure could be interpreted as diapause by environmental stochasticity, which seems to end after an unknown period of time after failure.…”
Section: Discussionmentioning
confidence: 99%
“…Fecal samples were individually extracted in a final volume of 200 μL, using conditions already described by manufacturers of the EZNA Blood DNA kit (OMEGA BIO-TEK, Norcross, GA, USA). T. cruzi presence was tested three times by PCR analysis directed to minicircle kDNA with oligos 121 and 122, using different volumes of extracted DNA as templates in a final volume of 50 μL to improve detection [ 47 , 48 ]. A volume of 10 μL of each reaction mixture was run in agarose gel electrophoresis (Lafken, Santiago, Chile) and stained with ethidium bromide (Invitrogen, Waltham, MA, USA).…”
Section: Methodsmentioning
confidence: 99%