Trypanosoma cruzi high infectivity in vitro is related to cardiac lesions during long-term infection in Beagledogs

Guedes, Paulo MM; Veloso, Vanja Maria; Caliari, Marcelo Vidigal; Carneiro, Cláudia Martins; Souza, Sheler M; Lana, Marta de; Chiari, Égler; Bahia, Maria T.; Galvão, Lúcia Maria da Cunha

doi:10.1590/s0074-02762007000200003

Cited by 31 publications

(27 citation statements)

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“…Lesions observed in chronic chagasic cardiomyopathy (CCC) frequently produce electrocardiographic alterations and affect cardiac function. The abnormalities recorded in the EKGs agreed with the cardiomegaly found in the infected dogs and the TcSP gene-immunized/infected group, this is consistent with reports in studies with Beagle dogs infected with other T. cruzi strains, where mild splenomegalies and severe cardiomegalies were observed in chronic CD [18,31]. In contrast, the dogs immunized with the TcSSP4 gene at the same dose and then infected exhibited a heart index similar to that of the healthy non-immunized group, and probably TcSSP4, the amastigote specific protein, encoded by the TcSSP4 gene, had a partial protective role during CCC likely because this gene induces immunity against the stagespecific surface antigen present on the intracellular form.…”

Section: Discussionsupporting

confidence: 90%

“…The cardiomegaly, inflammation, and fibrosis were associated to the presence of high in vitro infectivity of T. cruzi strains. Despite that Ninoa T. cruzi is a strain of low virulence, it was possible to find heart and spleen histological changes suggestive of cardiomegaly, myocarditis, and splenomegaly, as reported by other authors [18,28,31,33,34].…”

Section: Discussionsupporting

confidence: 56%

“…Cardiomegaly and splenomegaly were evaluated by determining the heart and spleen indices (organ weight/total body weight × 100), respectively, and also by inspecting for macroscopic alterations. Cardiomegaly and splenomegaly were considered present in the animals when the organ index was significantly higher than that observed in healthy non-infected animals [31]. .…”

Section: Macroscopic Evaluationmentioning

confidence: 99%

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Effect of the Plasmid-DNA Vaccination on Macroscopic and Microscopic Damage Caused by the Experimental ChronicTrypanosoma cruziInfection in the Canine Model

Rodríguez-Morales

Carrillo-Sánchez

García-Mendoza

et al. 2013

BioMed Research International

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The dog is considered the main domestic reservoir for Trypanosoma cruzi infection and a suitable experimental animal model to study the pathological changes during the course of Chagas disease (CD). Vaccine development is one of CD prevention methods to protect people at risk. Two plasmids containing genes encoding a trans-sialidase protein (TcSP) and an amastigote-specific glycoprotein (TcSSP4) were used as DNA vaccines in a canine model. Splenomegaly was not found in either of the recombinant plasmid-immunized groups; however, cardiomegaly was absent in animals immunized only with the plasmid containing the TcSSP4 gene. The inflammation of subendocardial and myocardial tissues was prevented only with the immunization with TcSSP4 gene. In conclusion, the vaccination with these genes has a partial protective effect on the enlargement of splenic and cardiac tissues during the chronic CD and on microscopic hearth damage, since both plasmids prevented splenomegaly but only one avoided cardiomegaly, and the lesions in heart tissue of dog immunized with plasmid containing the TcSSP4 gene covered only subepicardial tissue.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 56%

Section: Macroscopic Evaluationmentioning

confidence: 99%

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Effect of the Plasmid-DNA Vaccination on Macroscopic and Microscopic Damage Caused by the Experimental ChronicTrypanosoma cruziInfection in the Canine Model

Rodríguez-Morales

Carrillo-Sánchez

García-Mendoza

et al. 2013

BioMed Research International

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“…Tais achados assemelham-se ao registrado por Guedes et al (2009), que demonstraram que, diferentemente da fase aguda, todos os cães mostraram normalização dos nódulos linfáticos cervicais, fato também relatado por Andrade & Andrade (1980) e Klein & Camacho (1997). Essa ausência de sintomatologia clínica pode ser justiϐicada pelo desconhecimento do período de infecção natural, virulência dependente do tipo de cepa circulante e pela idade dos animais (Guedes et al 2007, Souza et al 2008. A idade média dos cães observada nesta pesquisa correspondia a animais adultos, portanto, torna-se mais diϐícil que os mesmos desenvolvam a fase aguda da doença à semelhança que ocorre em animais jovens, apresentando, assim, sinais menos evidentes (Camacho et al 2003).…”

Section: Resultsunclassified

Caracterização clínica e laboratorial de cães naturalmente infectados com Trypanosoma cruzi no semiárido nordestino

et al. 2012

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This study aimed to evidence the clinical and laboratorial signs of this disease to help characterize this illness in a natural way in the semiarid in the northeastern region. We evaluated 10 positive for Trypanosoma cruzi dogs, that were identiϐied by serological analysis of immunoϐluorescence assay (RIFI) and enzyme linked immunosorbent assay (ELISA); molecular analysis by polymerase chain reaction (PCR), direct microscopy and blood culture. The chagasic dogs underwent physical examination, electrocardiographic, radiographic, blood pressure, hematology (erythrocyte and leukocyte count) and biochemical exams (urea, creatinine, ALT, AST, PT, albumin, globulin, CK, CK-MB, and cTnl). The physical examination and the blood pressure were presented within the normal range, while in the electrocardiography the FC was observed as normal with a sinus rhythm, with the exception of one dog that presented a sinus tachycardia (168 bat/min). In the ECG of eight dogs there was increase of duration of P (47+6.5ms) suggestive to atrial enlargement, not conϐirmed in the radiography. A supraunlevelling was observed in the ST segment in one dog. In the hematological results, thrombocytopenia (187.4x10 3 +137.2x10 3 ) and anemia (5.0x10 6 +1.39x10 6 /ul) were noted. The mean hemoglobin (11 +2.7g/dL), hematocrit (34+10.5%) were below normal limits. The white series were within normal variation, with the exception of eosinophilia observed in three dogs. Individually, there were two dogs which registered leukocytosis, lymphocytosis and neutrophilia. In the biochemical evaluation there was hyperproteinemia PT=7.2 +0.9g/dL, hypoalbuminemia (2.2+0.4g/dL), hyperglobulinemia (5.1+1.0g/dL), increased of CK (196+171 U/L) and there was no alteration on ALT and AST enzymes. The CK-MB isoenzymes and cTnI did not change, except in three dogs. We conclude that dogs naturally infected in the northeastern semiarid present characteristics related to indeterminate chronic form (asymptomatic dogs) and that the identiϐication of the naturally infected dogs with no pathognomonic characteristics of the Chagas disease underscores the importance of this illness in the diagnostic process with the other proϐiles that show nonspeciϐic or not associated to cardiovascular disease.INDEX TERMS: Heart disease, parasite, Trypanosoma cruzi, Chagas disease, diseases of dog, parasitology.

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“…Triplicate samples were treated with NO donors as described earlier. Culture media were removed 48 h post‐infection and stained with Giemsa, according to the procedure previously described (Guedes et al. , 2007; Silva et al.…”

Section: Methodsmentioning

confidence: 99%

Novel ruthenium complexes as potential drugs for Chagas's disease: enzyme inhibition and in vitro/in vivo trypanocidal activity

Silva

Guedes

Zottis

et al. 2010

British J Pharmacology

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Background and purpose:The discovery of the pharmacological functions of nitric oxide has led to the development of NO donor compounds as therapeutic agents. A new generation of ruthenium NO donors, cis-[Ru(NO) , yielded survival rates of 80 and 60%, respectively, in infected mice, and eradicated any amastigotes from their myocardial tissue. Conclusions and implications:The ruthenium compounds exhibited potent in vitro and in vivo trypanocidal activities at doses up to 1000-fold lower than the clinical dose for benznidazole. Furthermore, one mechanism of action of these compounds is via the S-nitrosylation of Cys166 of T. cruzi GAPDH. Thus, these compounds show huge potential as candidates for the development of new drugs for the treatment of Chagas's disease.

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Trypanosoma cruzi high infectivity in vitro is related to cardiac lesions during long-term infection in Beagledogs

Cited by 31 publications

References 10 publications

Effect of the Plasmid-DNA Vaccination on Macroscopic and Microscopic Damage Caused by the Experimental ChronicTrypanosoma cruziInfection in the Canine Model

Effect of the Plasmid-DNA Vaccination on Macroscopic and Microscopic Damage Caused by the Experimental ChronicTrypanosoma cruziInfection in the Canine Model

Caracterização clínica e laboratorial de cães naturalmente infectados com Trypanosoma cruzi no semiárido nordestino

Novel ruthenium complexes as potential drugs for Chagas's disease: enzyme inhibition and in vitro/in vivo trypanocidal activity

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