Trypanosoma cruzi diversity in the Gran Chaco: Mixed infections and differential host distribution of TcV and TcVI

Monje-Rumi, María M.; Brandán, Cecilia Pérez; Ragone, Paula G.; Tomasini, Nicolás; Lauthier, Juan José; D’Amato, Anahí M. Alberti; Cimino, Rubén O.; Orellana, Viviana; Basombrío, Miguel Á.; Diosque, Patricio

doi:10.1016/j.meegid.2014.11.001

Cited by 56 publications

(48 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The recent detection of a TcIII-infected human case in the Argentine Chaco is consistent with this chain of evidence(Monje-Rumi et al, 2015). Rare findings of TcIII in dogs were also reported elsewhere(Chapman et al, 1984;.…”

supporting

confidence: 86%

“…The most recent, largest survey conducted in the same region yielded 115 DTU identifications from PCR-positive human and dog blood samples typed directly by PCR-DNA blotting and hybridization assays (Monje-Rumi et al, 2015): TcV predominated in humans and TcVI in dogs, as in previous studies, but nearly half (40/81) of human cases had mixed TcV-TcVI infections. We estimated that mixed infections with TcV-TcVI were significantly more frequent than expected from chance alone (χ 2 = 4.37, 1 df, P < 0.05).…”

Section: Molecular Epidemiology Of Trypanosoma Cruzi In the Argentinementioning

confidence: 69%

See 1 more Smart Citation

Reservoir host competence and the role of domestic and commensal hosts in the transmission of Trypanosoma cruzi

2015

View full text Add to dashboard Cite

supporting

confidence: 86%

Section: Molecular Epidemiology Of Trypanosoma Cruzi In the Argentinementioning

confidence: 69%

Reservoir host competence and the role of domestic and commensal hosts in the transmission of Trypanosoma cruzi

2015

View full text Add to dashboard Cite

“…cruzi has led to a great advance in the understanding of parasite population dynamics and epidemiology of the infection [26–30]. TcV is the most frequent genotype reported in infected humans of Argentina, followed by TcVI and TcI [11,31]. In the present study we have established 7 new human-derived T .…”

Section: Discussionmentioning

confidence: 89%

Phenotypic diversity and drug susceptibility of Trypanosoma cruzi TcV clinical isolates

et al. 2018

View full text Add to dashboard Cite

Trypanosoma cruzi is a genetically heterogeneous group of organisms that cause Chagas disease. It has been long suspected that the clinical outcome of the disease and response to therapeutic agents are, at least in part, related to the genetic characteristics of the parasite. Herein, we sought to validate the significance of the genotype of T. cruzi isolates recovered from patients with different clinical forms of Chagas disease living in Argentina on their biological behaviour and susceptibility to drugs. Genotype identification of the newly established isolates confirmed the reported predominance of TcV, with a minor frequency of TcI. Epimastigote sensitivity assays demonstrated marked dissimilar responses to benznidazole, nifurtimox, pentamidine and dihydroartemisinin in vitro. Two TcV isolates exhibiting divergent response to benznidazole in epimastigote assays were further tested for the expression of anti-oxidant proteins. Benznidazole-resistant BOL-FC10A epimastigotes had decreased expression of Old Yellow Enzyme and cytosolic superoxide dismutase, and overexpression of mitochondrial superoxide dismutase and tryparedoxin- 1, compared to benznidazole-susceptible AR-SE23C parasites. Drug sensitivity assays on intracellular amastigotes and trypomastigotes reproduced the higher susceptibility of AR-SE23C over BOL-FC10A parasites to benznidazole observed in epimastigotes assays. However, the susceptibility/resistance profile of amastigotes and trypomastigotes to nifurtimox, pentamidine and dihydroartemisinin varied markedly with respect to that of epimastigotes. C3H/He mice infected with AR-SE23C trypomastigotes had higher levels of parasitemia and mortality rate during the acute phase of infection compared to mice infected with BOL-FC10A trypomastigotes. Treatment of infected mice with benznidazole or nifurtimox was efficient to reduce patent parasitemia induced by either isolate. Nevertheless, qPCR performed at 70 dpi revealed parasite DNA in the blood of mice infected with AR-SE23C but not in BOL-FC10A infected mice. These results demonstrate high level of intra-type diversity which may represent an important obstacle for the testing of chemotherapeutic agents.

show abstract

“…This study has shown for the first time that TcIII and TcIV are related to human infection in ES. Until now, these DTUs have only been reported in the Amazon—TcIII and TcIV [12, 13], in Bolivian patients in Spain-TcIV [79], in the Southern and Northeast parts of Brazil—TcIII [91, 92], in Minas Gerais state-TcIII [93] and in Argentina-TcIII [94]. These findings show that the distribution of TcIII and in particular TcIV is higher than has been assumed up to now and confirm that these two DTUs are involved in human infection.…”

Section: Discussionmentioning

confidence: 99%

Ecological scenario and Trypanosoma cruzi DTU characterization of a fatal acute Chagas disease case transmitted orally (Espírito Santo state, Brazil)

et al. 2016

View full text Add to dashboard Cite

BackgroundTrypanosoma cruzi infection via oral route results in outbreaks or cases of acute Chagas disease (ACD) in different Brazilian regions and poses a novel epidemiological scenario. In the Espírito Santo state (southeastern Brazil), a fatal case of a patient with ACD led us to investigate the enzootic scenario to avoid the development of new cases. At the studied locality, Triatoma vitticeps exhibited high T. cruzi infection rates and frequently invaded residences.MethodsSylvatic and domestic mammals in the Rio da Prata locality, where the ACD case occurred, and in four surrounding areas (Baia Nova, Buenos Aires, Santa Rita and Todos os Santos) were examined and underwent parasitological and serological tests. Triatomines were collected for a fecal material exam, culturing and mini-exon gene molecular characterization, followed by RFLP-PCR of H3/Alul. Paraffin-embedded cardiac tissue of a patient was washed with xylene to remove paraffin and DNA was extracted using the phenol-chloroform method. For genotype characterization, PCR was performed to amplify the 1f8, GPI and 18S rRNA genes. In the case of V7V8 SSU rRNA, the PCR products were molecularly cloned. PCR products were sequenced and compared to sequences in GenBank. Phylogenetic analysis using maximum likelihood method with 1000 bootstrap replicates was performed.ResultsNone of the animals showed positive hemocultures. Three rodents and two dogs showed signs of infection, as inferred from borderline serological titers. T. vitticeps was the only triatomine species identified and showed T. cruzi infection by DTUs TcI and TcIV. The analysis of cardiac tissue DNA showed mixed infection by T. cruzi (DTUs I, II, III and IV) and Trypanosoma dionisii.ConclusionsEach case or outbreak of ACD should be analyzed as a particular epidemiological occurrence. The results indicated that mixed infections in humans may play a role in pathogenicity and may be more common than is currently recognized. Direct molecular characterization from biological samples is essential because this procedure avoids parasite selection. T. dionisii may under certain and unknown circumstances infect humans. The distribution of T. cruzi DTUS TcIII and TcIV in Brazilian biomes is broader than has been assumed to date.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1754-4) contains supplementary material, which is available to authorized users.

show abstract

Trypanosoma cruzi diversity in the Gran Chaco: Mixed infections and differential host distribution of TcV and TcVI

Cited by 56 publications

References 27 publications

Reservoir host competence and the role of domestic and commensal hosts in the transmission of Trypanosoma cruzi

Reservoir host competence and the role of domestic and commensal hosts in the transmission of Trypanosoma cruzi

Phenotypic diversity and drug susceptibility of Trypanosoma cruzi TcV clinical isolates

Ecological scenario and Trypanosoma cruzi DTU characterization of a fatal acute Chagas disease case transmitted orally (Espírito Santo state, Brazil)

Contact Info

Product

Resources

About