Trypanocidal potentials of african woody plants: In vitro trial of Khaya grandifoliola seed extracts against Trypanosoma brucei brucei

Owolabi, O. A.; Makanga, B.; Thomas, Emrys W.; Molyneux, David; Oliver, R.

doi:10.1016/0378-8741(90)90012-i

Cited by 13 publications

(8 citation statements)

References 8 publications

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“…That some of the plants tested showed promising trypanocidal effect (table 1) is not surprising since earlier reports (Freiburghaus et al, 1996(Freiburghaus et al, , 1997(Freiburghaus et al, , 1998Asuzu and Chineme, 1990;Nok et al, 1993;Owolabi et al, 1990) have clearly indicated that plants of different families could possess potent trypanocidal activity. In fact, natural products with trypanocidal activity and belonging to a variety of phytochemical classes have been identified (Hopp et al, 1976;Oliver-Bever, 1986;Sepulveda-Boza and Cassels, 1996).…”

Section: Discussionmentioning

confidence: 63%

In vitro trypanocidal effect of methanolic extract of some Nigerian savannah plants

Atawodi

Bulus

Ibrahim

et al. 2003

Afr. J. Biotechnol.

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Methanol extracts from twenty three plants harvested from the Savannah vegetation belt of Nigeria were analyzed in vitro for trypanocidal activity against Trypanosoma brucei brucei and Trypanosoma congolense at concentrations of 4 mg/ml, 0.4 mg/ml and 0.04 mg/ml. Extracts of Khaya senegalensis, Piliostigma reticulatum, Securidaca longepedunculata and Terminalia avicennoides were strongly trypanocidal to both organisms while extracts of Anchomanes difformis, Cassytha spp, Lannea kerstingii, Parkia clappertioniana, Striga spp, Adansonia digitata and Prosopis africana were trypanocidal to either T. brucei brucei or T. congolense. These findings provide evidence of the effects of some plants in the traditional management of trypanosomiasis.

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Section: Discussionmentioning

confidence: 63%

In vitro trypanocidal effect of methanolic extract of some Nigerian savannah plants

Atawodi

Bulus

Ibrahim

et al. 2003

Afr. J. Biotechnol.

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“…Results of this study showed that the extracts of some of the plants (Terminalia catappa, Waltheria indica, Cucubita pepo, Entada abyssinica and Ximenia americana) had strong trypanocidal activity in vitro, while the extracts of Stylosanthes erecta and Holarrhena floribunda had moderate trypanocidal activity, with extract of Cissus quadrangularis showing no in vitro effect. That some of the plants showed promising trypanocidal effect is not surprising, since earlier reports (Asuzu and Chineme, 1990;Owolabi et al, 1990;Nok et al, 1993;Freiburghaus et al, 1996Freiburghaus et al, , 1997Freiburghaus et al, , 1998Bala et al, 2005;Agbedahunsi et al, 2006) have clearly indicated that plants of different families could possess potent trypanocidal activity. In fact, natural products with trypanocidal activity and belonging to a variety of phytochemical classes have been identified (Hopp et al, 1976;Sepulveda -Boza and Cassels, 1996;Oliver -Bever, 1986).…”

Section: Discussionmentioning

confidence: 78%

Studies on the In Vitro Trypanocidal Effect of the Extracts of Some Selected Medicinal Plants in Sokoto State, Nigeria

Bala¹,

Adamu²,

Abubakar³

et al. 2010

Nig J Bas App Sci

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The in vitro trypanocidal activity of aqueous extracts of some selected medicinal plants used by local herdsmen in the treatment of various animal diseases in Sokoto State, Nigeria was conducted. Trypanosoma brucei brucei were cultured using 96 well micro titer plate and maintained at 37 0 C. About 20-25 parasites per microscope field were dosed with 1, 2 and 4mg/ml of aqueous extracts of the plants and a control group without extracts. After 5 minutes incubation in Eppendorf tubes maintained at 37 0 C, the parasites survived more than four (4) hours in the absence of extract/Berenil. At 4mg/ml of the extracts 0f Terminalia catappa, Waltheria indica, Cucurbita pepo, Entada abyssinica and Ximenia Americana, complete cessation of motility of T. brucei brucei within 60 minutes was observed. However, at 2mg/ml of Waltheria indica, trypanosome motility after 20 minutes was stopped but Terminalia catappa, and Ximenia americana were found to reduce trypanosome motility at 35 and 55 minutes respectively. Only Waltheria indica reduced trypanosome motility within 25 minutes at 1mg/ml concentration. Berenil, the standard drug, caused cessation of trypanosomal motility within 60 minutes even at 1mg/ml. From the results Waltheria indica was the most effective among the extracts when compared with Berenil, and may be a potential source of compounds with trypanocidal activity.

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“…In Nigeria, the indigenous people are exploiting a variety of herbs for ef-fective curing of various ailments [12]. In Nigeria, several ethnobotanical studies of Nigerian plants used in the traditional management of trypanosomiasis indicated both significant in vitro /in vivo antitrypanosomal activity [13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Medicinal Plants from Nupeland for Their in vivo Antitrypanosomal Activity

Mann¹,

Ogbadoyi²

2012

AJB

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Over 25% of our common medicines contain at least some compounds obtained from plants. Nupeland located on the coastal basins of Rivers Niger and Kaduna in North Central Nigeria is greatly influenced by the savannah-forest vegetations with biodiversity rich in medicinal plants. This type of vegetation also harbours tsetsefly which are the vector of trypanosomes, thus making the area highly endemic to sleeping sickness. Trypanosomiasis is a tropical disease threatening human health and economical development and is responsible for the death of about half a million patients per year. Nupeland inhabited predominantly by Nupe tribe are traditionally noted for generations of using medicinal plants in curing human trypanosomal infections; many of which have not been scientifically proved. Natural products derived from plants offer novel possibilities to obtain new drugs that are active against trypanosomes. In furtherance to the earlier in vitro trypanocidal activity evaluation of some Nupeland medicinal plants; six medicinal plants traditionally used for treatment of sleeping sickness in Nupeland namely: Acacia nilotica, Bombax buonopozense, Heterotis rotundifolia, Pterocarpus erinaceus, Terminalia avicennioides and Zanthoxylum zanthoxyloides were investigated for in vivo antitrypanosomal activity. The solvent soluble extracts obtained by standard methods from various plant parts (stem barks and fruits) of Nupeland medicinal plants were evaluated for their in vivo antitrypanosomal activities against Trypanosoma brucei brucei as well as their phytochemical screening were performed by standard procedures. The extracts of A. nilotica (stem bark), B. buonopozense (stem bark), H. rotundifolia (whole plant), T. avicennioides (round fruit) and Z. zanthoxyloides (stem bark) were effective on trypanosomes. The extracts of A. nilotica, B. buonopozense and H. rotundifolia exhibited antitrypanosomal effects at 200, 300 and 800 mg/kg body weight respectively and cleared the parasites from circulation with prolonging survival period of up to 30 days. The stem bark extracts of P. erinaceus, T. avicennioides and Z. zanthoxyloides showed only trypanostatic effects and could not clear the parasites completely. These extracts contain metabolites that are associated antitrypanosomal effects; hence these medicinal plants may be sources of new antitrypanosomal compounds that may be active against T. b. brucei. The discovery of these potent antitrypanosomal extracts from these plants has increased their potentials to provide lead compounds for the development of new natural drugs for effective treatment of sleeping sickness. It has also justified the claim that some medicinal plants of Nupeland possess antitrypanosomal activity and could be useful in the management of trypanosomiasis. The isolation of trypanocidal compounds from these extracts will require State-Art-Instrumentation particularly for further bioactive fractionation and characterization using chromatographic and spectroscopic techniques.

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Trypanocidal potentials of african woody plants: In vitro trial of Khaya grandifoliola seed extracts against Trypanosoma brucei brucei

Cited by 13 publications

References 8 publications

In vitro trypanocidal effect of methanolic extract of some Nigerian savannah plants

In vitro trypanocidal effect of methanolic extract of some Nigerian savannah plants

Studies on the In Vitro Trypanocidal Effect of the Extracts of Some Selected Medicinal Plants in Sokoto State, Nigeria

Evaluation of Medicinal Plants from Nupeland for Their in vivo Antitrypanosomal Activity

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