In vitro trypanocidal effect of methanolic extract of some Nigerian savannah plants

Atawodi, Sunday E.; Bulus, T.; Ibrahim, S.; Ameh, D. A.; Nok, AJ; Mamman, M.; Galadima, M

doi:10.5897/ajb2003.000-1065

Cited by 99 publications

(6 citation statements)

References 18 publications

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“…Aside from being a plant that is super-low in calories and high in nutrients like vitamins A, C and manganese, M. oleifera contains an anti-oxidant called chlorogenic acid. This anti-oxidant regulates blood sugar and acts as a fat burner thereby enhancing weight loss (Atawodi et al, 2003), which may have been the case in the present study, and irrespective of the presence of Aluminium chloride.…”

Section: Discussionsupporting

confidence: 59%

Effect of Moringa oleifera Lam. Leaf Extract Against Aluminium Chloride Induced Hippocampal Histology and Serum Enzyme activities in adult Wistar rats

Adighije

Ekerete

Ekong

2020

IJMSS

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Introduction: Aluminium, a ubiquitous metal implicated in some neurodegenerative diseases is linked to activation of free oxygen species. The antioxidant-rich plants, Moringa oleifera (MO) is reported to protect against Aluminium activities. This study investigated the actions of MO leaf extract (MOLE) against Aluminium chloride (AlCl3 )- induced hippocampal cellular changes and serum levels of alkaline phosphatase (ALP), aspartate transaminase (AST) and alanine transaminase (ALT) in adult Wistar rats. Materials and Methods: Thirty Wistar rats weighing between 150 g and 220 g were grouped (n=5) into; 1-control (5 mL/kg distilled water), 2-AlCl3 (100 mg/kg), 3-low dose MOLE (250 mg/kg), 4-high dose MOLE (1,000 mg/kg), 5-concurrent AlCl3 and low dose MOLE, and 6-concurrent AlCl3 and high dose MOLE. All administrations were by oral gavages for 21 days. On day 22, following deep anaesthesia and cardiac puncture, blood was obtained for serum enzyme analysis, and the brain perfusion fixed, harvested and processed for histological study. Results: Results showed significantly (p < 0.05) higher ALP level in the AlCl3 group compared with the control, as well as the other test groups. However, there was no significant (p > 0.05) AST and ALT levels. The hippocampal CA3 of the AlCl3 group showed hypertrophic cells, with some of the cells having karyorrhectic features. The concurrent AlCl3 and low and high doses, MOLE groups showed less of these adverse features. Conclusion: These results suggest that MOLE may protect enzymatic activities against Aluminium chloride. However, its action on hippocampus is still subject to further investigation.

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Section: Discussionsupporting

confidence: 59%

Effect of Moringa oleifera Lam. Leaf Extract Against Aluminium Chloride Induced Hippocampal Histology and Serum Enzyme activities in adult Wistar rats

Adighije

Ekerete

Ekong

2020

IJMSS

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“…Thereafter, about 2 μl of the test mixture were placed on separate microscope slide and covered with cover slips and parasite observed after every 5 min for motility. Cessation or reduction in motility of the parasites in extract treated blood compared to that of the parasite loaded control blood without extract was taken as a measure of trypanocidal activity [25, 26]. …”

Section: Methodsmentioning

confidence: 99%

Trypanocidal activity of ethanolic extracts of Commiphora swynnertonii Burtt on Trypanosoma congolense

Nagagi

Silayo

Kweka

2016

BMC Complement Altern Med

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BackgroundAfrican trypanosomosis is the disease caused by extracellular protozoan parasites of the genus Trypanosoma transmitted by tsetse flies. The current study has evaluated the trypanocidal activity of Commiphora swynnertonii extracts on Trypanosoma congolense.MethodsThe effect of ethanolic stem bark and resinous extracts on motility of T. congolense was evaluated by in vitro study at concentrations of 2 mg/ml and 4 mg/ml. Then, trypanocidal activity was evaluated by drug incubation infectivity test using mice at concentrations of 0.4 mg/ml and 2 mg/ml. In both studies negative (without drug) and positive (diminazene diaceturate) controls were used.ResultsThe in vitro study showed that, ethanolic stem bark extract of C. swynnertonii at concentration of 4 mg/ml caused complete cessation of motility for T. congolense in 30 min. However, resinous ethanolic extract had delayed effect on cessation of motility of T. congolense observed at 90 and 100 min post-incubation at concentrations of 4 mg/ml and 2 mg/ml respectively. The drug incubation infectivity test study depicted that ethanolic stem bark extract at concentration of 2 mg/ml significantly (p = 0.000) reduced the infectivity of T. congolense in mice. However, it did not vary significantly (P =0.897) with group treated with diminazene diaceturate incubated mixture.ConclusionThe current study has provided evidence that, ethanolic stem bark extract of C. swynnertonii possess trypanocidal activity against T. congolense. Based on these findings, further studies are recommended to determine its potential as a lead to trypanocidal drug discovery.

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“…The protocols of Atawodi et al, (2003) were adopted by weighing 200mg of the Allium sativum aqeuoes extract disolved in 5mls of phosphate buffered saline solution (PBSS) giving 40mg/ml concentration, followed by serial dilution of the stock using PBSS to obtain descending grades of 40.0, 20.0, 10.0, 5.0, 2.5, 1.25, 0.625, 0.313, 0.156 and 0.078 mg concentrations. Trypanasoma brucei brucei Federe strain/NTR/14 obtained from the Nigerian Institute for Trypanosomiasis and Onchocerciasis Research (NITOR) Vom, Plateau State, Nigeria, stabilized through serial passage in donor albino rats and identified based on their morphology and negative blood inhibition infectivity test (BIT).…”

Section: In Vitro-testingmentioning

confidence: 99%

Phytochemistry, Invitro Anti-Trypanosomal Efficacy and Acute Toxicity of ‎Allium sativum Linn. Bulb Aqueous Extract

Biu,

Buratai,

Maina

et al. 2023

SJVS

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Allium sativum Linn. is a bulbos perennial plant of the Family Liliaceae has been reported as having potential medicinal properties. This study was on the phytochemistry, in vitro anti-trypanosomal efficacy and acute toxicity of Allium sativum Linn bulb aqueous extract using standard procedures. Qualitative phytochemistry indicated high scores (+++) for reducing sugars, moderate scores (++) for alkaloids, steriods and carbohydrates with low scores (+) for tannins, flavonoids and saponins. The median lethal dose of the aqueous extract in albino rats was determined to be 2400 mg/kg body weight following intraperitoneal administration. The albino rats exhibited weakness, lordosis, starry hair coat, awkward posture, loss of apetite. All the albino rats died within 24 hours. There was a significant (p<0.05) reduction in mean parasites count (x106) on exposure in vitro to all the graded extract concentrations compared with the normal, and parasite count also decreasedwith time post-inoculation. Similarly, a positive correlation existed between extract concentrations and inhibition rates of the parasites post-inoculation. In conclusion, the aqueous extract of Allium sativum has in vitro anti-trypanosomal activity.

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In vitro trypanocidal effect of methanolic extract of some Nigerian savannah plants

Cited by 99 publications

References 18 publications

Effect of Moringa oleifera Lam. Leaf Extract Against Aluminium Chloride Induced Hippocampal Histology and Serum Enzyme activities in adult Wistar rats

Effect of Moringa oleifera Lam. Leaf Extract Against Aluminium Chloride Induced Hippocampal Histology and Serum Enzyme activities in adult Wistar rats

Trypanocidal activity of ethanolic extracts of Commiphora swynnertonii Burtt on Trypanosoma congolense

Phytochemistry, Invitro Anti-Trypanosomal Efficacy and Acute Toxicity of ‎Allium sativum Linn. Bulb Aqueous Extract

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