Trypanocidal Constituents in Plants 3. Leaves of Garcinia intermedia and Heartwood of Calophyllum brasiliense

Abe, Fumiko; Nagafuji, Shinya; Okabe, Hikaru; Akahane, H.; Estrada-Muñiz, Elizabet; Huerta-Reyes, Maira; Reyes-Chilpa, Ricardo

doi:10.1248/bpb.27.141

Cited by 77 publications

(50 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to its HIV-inhibitory activity (Gustafson et al, 1992), guttiferone A (3) has also been shown to be a potent inhibitor of cholinesterase (Lenta et al, 2007) and glutathione transferase enzymes (Muleya et al, 2008), as well as exhibiting antioxidant (Ngouela et al, 2006), leishmanicidal (Lenta et al, 2007), antiplasmodial (Ngouela et al, 2006), trypanocidal (Abe et al, 2004), and cytotoxic (Williams et al, 2003) effects. The activity of morelloflavone (1) as an antiinflammatory (Gil et al, 1997;Castardo et al, 2008) and antioxidant (Sanz et al, 1994;Deachathai et al, 2005;Hutadilok-Towatana et al, 2007) are well-documented; it has also been found to be a potent tyrosinase inhibitor (Masuda et al, 2005), anti-HIV-1 (Lin et al, 1997), and anti-bacterial (Verdi et al, 2004) agent, and to have antimalarial (Ngouamegne et al, 2008) and analgesic (Luzzi et al, 1997) properties.…”

Section: Resultsmentioning

confidence: 99%

Non-toxic melanin production inhibitors from Garcinia livingstonei (Clusiaceae)

Mulholland

Mwangi

Dlova³

et al. 2013

Journal of Ethnopharmacology

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Non-toxic melanin production inhibitors from Garcinia livingstonei (Clusiaceae)

Mulholland

Mwangi

Dlova³

et al. 2013

Journal of Ethnopharmacology

View full text Add to dashboard Cite

“…MC 100 values of these compounds and berberine chloride 3) are listed in Table 2. IC 50 values against epimastigotes were estimated as described in the preceding paper 1) and are shown in Table 2.…”

Section: Resultsmentioning

confidence: 99%

“…Assay procedure against trypomastigotes was described in the previous paper. 3,4) The activity was expressed as the MC 100 value as in the case of epimastigotes after 24-incubation at 37°C under 5% CO 2 . IC 50 values were estimated using Cell Counting Kit-8 as described in the preceding paper.…”

Section: )mentioning

confidence: 99%

See 1 more Smart Citation

Trypanocidal Constituents in Plants 5. Evaluation of Some Mexican Plants for Their Trypanocidal Activity and Active Constituents in the Seeds of Persea americana

Abe

Nagafuji

Okawa

et al. 2005

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

As a preliminary screening test for the trypanocidal activity against epimastigotes of Trypanosoma cruzi in vitro, we examined some crude extracts of Mexican medicinal plants. 2)T. cruzi is the etiological agent for Chagas' disease (American trypanosomiasis), one of the most serious protozoan diseases in Latin America. Then we started on identification of the principal compounds of the active extracts seeking new chemotherapeutic agents for Chagas' disease. Lignans 2) from Guaco (roots of Aristolochia taliscana) and xanthones 3) from Bari (stems of Calophyllum brasiliense) were isolated as active components against epimastigotes in vitro.T. cruzi exhibits three developmental stages: epimastigote in the insect gut; trypomastigote, an infectious form in the mammalian blood stream; and an amastigote, a proliferative form in mammalian cells. In this paper we describe the results of continuous preliminary screening tests for trypanocidal activity against trypomastigotes as well as against epimastigotes in some Mexican plants.The edible fruit of Persea americana MILL. (Lauraceae), avocado, is grown throughout the tropics worldwides. The MeOH extract of seeds of Persea americana also showed moderate activity in the screening test. Herein we also describe the identification of the active constituents in seeds of P. americana. MATERIALS AND METHODS Plant Materials and Preparation of Their ExtractsPlant materials were mainly purchased at a medicinal plant market in Guadalajara, Jalisco, and collected in the fields. Identification of the plants was done by M. A. Martinez-Alfaro, one of the co-authors. The voucher specimens were deposited in the Herbarium of Jardín Botánico del Instituto de Biologia, Universidad Nacional Autonoma de Mexico.The plants examined are listed in Table 1 including those referred to in ref. 2. Dried and powdered materials were extracted with MeOH or MeOH-CH 2 Cl 2 at room temperature overnight. In the case of fresh materials, chopped materials were soaked in MeOH and filtered. The residue was extracted again with MeOH. The solvent was concentrated in vacuo to give each extract.Cultivation of T. cruzi The strain of T. cruzi used in this study and the cultivation method of epimastigotes were the same as those described in the previous paper.2) The trypomastigotes were harvested from the culture supernatant of rhesus monkey kidney (LLC-MK2) cells infected with T. cruzi as described in the previous paper.4,5) LLC-MK-2 cells were maintained in DMEM containing 10% FBS at 37°C under 5% CO 2 . After inoculation of trypomastigotes into LLC-MK2 cells, the medium was changed to DMEM containing 10% NCS.Instruments and Reagents The instruments and reagents used in this study are the same as those described in the preceding paper. 1)Trypanocidal Assay The detailed assay procedure against epimastigotes was described in the previous paper.2) The activity was expressed as the MC 100 value (mg/ml or mM), which was defined as the minimum concentration at which all the epimastigotes were terminated after incubation fo...

show abstract

“…Cell Counting Kit-8 In previous papers, 1,2,9) we assayed trypanocidal activity by observing the mobility of T. cruzi microscopically. The activity was shown by MC 100 value, which was defined as the minimum concentration at which all the epimastigotes or trypomastigotes become immobilized after 24 or 48 h incubation.…”

Section: Evaluation Of Ic 50 Values Against Epimastigotes Usingmentioning

confidence: 99%

Trypanocidal Constituents in Plants: 4. Withanolides from the Aerial Parts of Physalis angulata

Nagafuji

Okabe

Akahane

et al. 2004

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

In the previous paper in this series, 2) we described the identification of trypanocidal xanthones in Garcinia subelliptica against epimastigotes and trypomastigotes of the protozoan Trypanosoma cruzi, which is the etiologic agent of Chagas' disease (American trypanosomiasis). In the course of our search for trypanocidal constituents in plants, we have found that the MeOH extract of the aerial parts of Physalis angulata L. (Solanaceae) showed considerable activity.P. angulata is indigenous to tropical America and was naturalized in Japan in the Edo era. It is an annual small herb and has been traditionally utilized for antipyretic purposes in Japan.3) The isolation of ergostane-type steroidal compounds, physagulins A-G, 4-6) withangulatin A 7) and physalins, 8) has been reported from this plant.T. cruzi is transformed into three stages in its life cycle: epimastigote in the insect gut; trypomastigote, an infectious form in the mammalian blood stream; and amastigote, a proliferative form in mammalian cells. During the isolation procedure, trypanocidal activity was monitored against epimastigotes, because it is easy to cultivate them and they are not infectious. Ten withanolides (1-10) were isolated from the active fraction. To check the therapeutic possibility of these withanolides, trypanocidal activity against trypomastigotes, an infectious form, was examined. Furthermore, evaluation of the IC 50 value against epimastigotes was performed using Cell Counting Kit-8 in order to compare with cytotoxicity at the IC 50 value. In this paper we describe the structural elucidation of the four new withanolides (2, 5, 9, 10) and the trypanocidal activity of the isolates. The possibility of chemotherapeutic utility is also discussed. MATERIALS AND METHODSPlant Materials P. angulata was cultivated in the medicinal plant garden of Fukuoka University and harvested in September 2002. The aerial parts were air-dried and powdered.Instruments and Reagents The instruments used in this study were a JEOL JNM-A500 for NMR spectra (500 MHz for 1 H-NMR, 125 MHz for 13 C-NMR), a JEOL JMS-HX 110 for MS spectra, a JASCO DIP 360 polarimeter for optical rotations, and a Labsystems Multiskan JX Microplate Reader for measuring the absorbance of a formazan product. The following materials were used for chromatography: Diaion HP-20 (a styrene polymer, Mitsubishi Chemical Industries, Ltd.), Silica gel 60N (Cica), Sephadex LH-20 (Pharmacia Fine Chemicals), and YMC gel (ODS, YMC Co. Ltd.). Newborn calf serum (NCS) was purchased from Nacalai Tesque. Dulbecco's modified Eagle's medium (DMEM) and RPMI 1640 medium were purchased from Sigma. Gentian violet, ketoconazole, and 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Wako. A Cell Counting Kit-8 was purchased from Dojindo. Other reagents are the same as in the previous papers. 2,9)Cultivation of T. cruzi The strain of T. cruzi used in this study and the cultivation methods of epimastigotes and trypomastigotes were the same as those described in the previous p...

show abstract

Trypanocidal Constituents in Plants 3. Leaves of Garcinia intermedia and Heartwood of Calophyllum brasiliense

Cited by 77 publications

References 18 publications

Non-toxic melanin production inhibitors from Garcinia livingstonei (Clusiaceae)

Non-toxic melanin production inhibitors from Garcinia livingstonei (Clusiaceae)

Trypanocidal Constituents in Plants 5. Evaluation of Some Mexican Plants for Their Trypanocidal Activity and Active Constituents in the Seeds of Persea americana

Trypanocidal Constituents in Plants: 4. Withanolides from the Aerial Parts of Physalis angulata

Contact Info

Product

Resources

About