Trypanocidal Activity of a New Diterpene from<i>Casearia sylvestris</i>var.<i>lingua</i>

Espindola, Laïla Salmen; Júnior, José Rossy e Vasconcelos; Mesquita, Mariana Laundry de; Marquié, Patrick; Paula, José Elías de; Mambu, Lengo; Santana, Jaime M.

doi:10.1055/s-2004-832655

Cited by 60 publications

(60 citation statements)

References 9 publications

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“…Twenty eight clerodane diterpenes typical of Casearia were isolated from C. sylvestris: casearins A-X (Carvalho et al 1998, Itokawa et al 1990, Morita et al 1991, Wang et al 2009, Santos et al 2010 and caseargrewiin F (Santos et al 2010), isolated from leaves; casearvestrins A-C, isolated from leaves and branches (Oberlies et al 2002);and rel-19S-acetoxy-18R-butanoyloxy-18,19-epoxy-6S-hidroxy-2R-(2-methylbutanoyloxy) -5S, 8R, 9R, 10S-cleroda-3,13(16),14-triene, isolated from roots (Espíndola et al 2004) (Fig. 1).…”

Section: Antitumor Activitymentioning

confidence: 99%

“…Besides this antitumor potentiality, different parts of the plant also exhibit activity against Leishmania donovani promastigotes (Mesquita et al 2005), Trypanosoma cruzi amastigotes (Espíndola et al 2004, Mesquita et al 2005, Plasmodium falciparum strains resistant to chloroquine (Mesquita et al 2007) and Aedes aegypti larvae (Rodrigues et al 2006). Leaf methanolic extract shows anti-hyperlipidemic properties, decreasing serum triglycerides and lipases (Schoenfelder et al 2008) and the water extract displays neuromodulatory effects on central nervous system by the interference on enzymatic action of the purinergic and cholinergic systems since it inhibits Na + -/K + /ATPase and 5 -nucleotidase activities (Silva et al 2006).…”

Section: Antitumor Activitymentioning

confidence: 99%

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Folk uses and pharmacological properties of Casearia sylvestris: a medicinal review

Ferreira

Costa-Lotufo

Moraes

et al. 2011

An. Acad. Bras. Ciênc.

103

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Folk uses and scientific investigations have highlighted the importance of Casearia sylvestris extracts and their relevant bioactive potential. The aim of this work was to review the pharmacological properties of C. sylvestris, emphasizing its anti-ulcer, anti-inflammatory, anti-ophidian and antitumor potentialities. Ethanolic extracts and essential oil of their leaves have antiulcerogenic activity and reduce gastric volume without altering the stomach pH, which corroborates their consumption on gastrointestinal disorders. Leaf water extracts show phospholipase A 2 inhibitory activity that prevents damage effects on the muscular tissue after toxin inoculation. This antiphospholipasic action is probably related to the use as an anti-inflammatory, proposing a pharmacological blockage similar to that obtained with non-steroidal anti-inflammatory drugs on arachidonic acid and cyclooxygenase pathways. Bioguided-assay fractionations lead to the identification of secondary metabolites, especially the clerodane diterpenes casearins (A-X) and casearvestrins (A-C), compounds with a remarkable cytotoxic and antitumor action. Therefore, the C. sylvestris shrub holds a known worldwide pharmacological arsenal by its extensive folk utilization, exciting searches for new molecules and a better comprehension about biological properties.

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Section: Antitumor Activitymentioning

confidence: 99%

Section: Antitumor Activitymentioning

confidence: 99%

Folk uses and pharmacological properties of Casearia sylvestris: a medicinal review

Ferreira

Costa-Lotufo

Moraes

et al. 2011

An. Acad. Bras. Ciênc.

103

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“…Para ação antilarvativa foi realizado o extrato hexânico e etanólico, sendo testados in vitro, em doses de 500 ìg/mL, apresentando atividade larvativa contra Aedes aegypti (Rodrigues et al, 2006). Para ação antiprotozoária foi analisado o extrato hexânico da casca da raiz, sendo testado in vitro, em doses de 0,1 a 4,9 ìg/mL, apresentando atividade contra Trypanosoma cruzi (doença de Chagas) (Mesquita et al,2005;Espindola et al, 2004).…”

Section: Rodrigues Etunclassified

Casearia sylvestris SW. (SALICACEAE) E JACARANDA PUBERULA CHAM. (BIGNONIACEAE): USO POPULAR VERSUS LITERATURA CIENTÍFICA

Darabas

Moreira

Citadini-Zanette

et al. 2009

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A utilização de plantas medicinais é notória, entretanto, nem todas possuem ações terapêuticas cientificamente comprovadas. Este artigo é resultado de um levantamento bibliográfico sobre duas árvores medicinais presentes no Parque Ecológico Municipal José Milanese (PEMJM), localizado no Bairro Mina União, no município de Criciúma, Santa Catarina e utilizadas pela população local por apresentarem efeitos terapêuticos. Trata-se de uma revisão de cunho científico sobre os efeitos terapêuticos apontados pela comunidade e posteriormente confrontados em periódicos reconhecidos pela comunidade científica. Foram estudadas Casearia sylvestris Sw.(Salicaceae) e Jacaranda puberula Cham. (Bignoniaceae) nas base de dados eletrônicas Pubmed e Science Direct, cujas informações foram fundamentais para comparar seus possíveis usos medicinais. Pode-se observar que alguns relatos populares vêm ao encontro das informações obtidas nesses bancos de dados, e outras extrapolam as informações populares.

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“…At least 16 Casearia species have shown biological activity such as cytotoxic [C. arborea; C. grayi; C. grewiifolia; C. lucida; C. multinervosa; C. nigrescens; C. obliqua (2)(3)(4)(5)(6)(7)(8)]; antifungal [C. decandra (9)]; antidiabetic [C. esculenta (10)]; immunomodulatory [C. guianensis (11)]; antifertility [C. ilicifolia (12)]; antiophidian [C. mariquitensis (13)]; antitumor [C. membranacea (14)]; genotoxic [C. tomentosa (15)]; and antioxidant actions [C. velutina (16)]. The most studied among them is Casearia sylvestris Sw. that has shown several biological activities, including antitumor, antiulcer, analgesic, anti-inflammatory, cytotoxic, antifungal, anticancer, anti-snake and bee venom PLA 2 , genotoxic, trypanocidal, enzyme inhibitory, antihyperlipidemic and antibothropic properties (17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27). The latter have been used as antivenom in folk medicine by isolated Brazilian communities (22).…”

Section: Introductionmentioning

confidence: 99%

Bothrops jararacussu venom-induced neuromuscular blockade inhibited by Casearia gossypiosperma Briquet hydroalcoholic extract

Camargo¹,

Nazato²,

Silva³

et al. 2010

J. Venom. Anim. Toxins incl. Trop. Dis

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Abstract:The hydroalcoholic extract of Casearia gossypiosperma Briquet (Flacourtiaceae) was standardized for the first time through quality control procedures including pharmacognostic methods, fingerprint chromatograms, defined amounts of marker substances and physicochemical characteristics. The pharmacological activity of C. gossypiosperma (Cg) hydroalcoholic extract was assayed by a traditional in vitro test, which involved irreversible neuromuscular blockade induced by Bothrops jararacussu (Bjssu) venom (60 μg/mL) in mouse phrenic nerve-diaphragm preparations. Bjssu venom blocked muscle activity for 26 (± 2.0) minutes (n = 6). Cg extract (0.1 mg/mL) induced changes on the baseline muscle activity without impairing the muscle function and inhibited 87.6% (± 1.8) (n = 6) of the Bjssu venom-induced blockade. Both flavonoids (0.624 g%) and polyphenols (4.63 g%) from the extract were spectrophotometrically quantified. Therefore, the present study confirms the antibothropic activity of Cg extract, supporting the ethnomedical use of Casearia sp. in the treatment of snakebite victims.Key words: antibothropic extract, Bothrops jararacussu, Casearia gossypiosperma Briquet, snake venoms. pharmacologically assessed for the first time. Original PaPer The Journal of Venomous Animals and Toxins including Tropical DiseasesIn vivo myotoxic effects and in vitro irreversible neuromuscular blockade effects of crude venom from the snake Bothrops jararacussu are well known pharmacological methods used to study drugs showing antivenom properties (29,30). Thus, the aim of the present study was to verify the capability of Casearia gossypiosperma hydroalcoholic extract to neutralize the neuromuscular blockade induced by Bothrops jararacussu venom. MATERIALS AND METHODS Plant Material and ExtractionLeaves from an adult C. gossypiosperma Briquet (Cg) tree were collected from the herbarium at the Higher School of Agriculture Luiz de Queiroz (ESALQ) of the University of São Paulo, Piracicaba, São Paulo, Brazil, in January 2006; the species was previously identified in 1988 (protocol IAC 38115). Cg leaves were dried at 35°C to 40°C for 24 hours. The dried leaves were then powdered, ground in a mill, macerated (200 g, during five days) in 2 L of 70% ethanol and the suspension was percolated (under protection against light) at 20 drops/minute, resulting in a 10% (m/v) hydroalcoholic extract. Quality Control Assays of the Medicinal Plants Ash and humidity testsTo observe elementary physical and chemical characteristics, Cg powder was subjected to ash and humidity tests (31). Briefly, 100 g of the specimen powder were placed in six calibrated melting pots, which were warmed until total carbonization of the powders. The melting pots were kept at 650°C and the ashes were then weighed. Results are expressed as grams of ashes/100 g of sample. The humidity test was performed by placing 1 g of specimen powder in six calibrated porcelain capsules, which were heated at 105ºC during four hours and then weighed. Thin Layer Chromatography (TLC)Aliquots...

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Trypanocidal Activity of a New Diterpene fromCasearia sylvestrisvar.lingua

Cited by 60 publications

References 9 publications

Folk uses and pharmacological properties of Casearia sylvestris: a medicinal review

Folk uses and pharmacological properties of Casearia sylvestris: a medicinal review

Casearia sylvestris SW. (SALICACEAE) E JACARANDA PUBERULA CHAM. (BIGNONIACEAE): USO POPULAR VERSUS LITERATURA CIENTÍFICA

Bothrops jararacussu venom-induced neuromuscular blockade inhibited by Casearia gossypiosperma Briquet hydroalcoholic extract

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