Truncation of βA3/A1-crystallin during Aging of the Bovine Lens; Possible Implications for Lens Optical Quality

Analysis of UVA-Related Alterations upon Aging of Eye Lens Proteins by Mini Two-Dimensional Polyacrylamide Gel Electrophoresis

“…Age-related alterations have been investigated by Werten et al [35] and Lampi et al [36]. In the present study, we have identified age-related modifications and changes of ·A-crystallin and one of the major urea-soluble cytoskeletal lens proteins following irradiation.…”

Purification and Characterization of Lens Specific Calpain (Lp82) from Bovine Lens

“…bA3-crystallin missing 11 amino acids from its N-terminus is also found in vivo in both young cow and rodent lenses (David et al, 1994), but undergoes removal of an additional 11 residues during further aging (Werten, Vos and De Jong, 1999). While the initial cleavage may be produced by either or both enzymes in vivo, the protease responsible for the additional cleavage remains unknown.…”

Pb2+ exposure alters the lens αA-crystallin protein profile in vivo and induces cataract formation in lens organ culture

“…Abnormal modifications of the crystallins may contribute to the development of cataracts (Werten et al, 1999;David et al, 1994;Lampi et al, 1998) through altered protein-protein interactions including altered chaperoning activity and protein packing. The present study examined the ability of shortterm oral Pb 2+ exposure to alter the post-translational protein expression profile of a dominant lens chaperone protein isoform, ␣A-crystallin and of another crystallin, ␤A 4 -crystallin.…”