TRPV1 Is Associated with Testicular Apoptosis in Mice

Abstract

Reproductive potential decreases with age. A decrease in male fertility is due to a combination of morphological and molecular alterations in the testes. Transient receptor potential vanilloid receptor-1 (TRPV1) is associated with aging and lifespan, and its activation causes apoptotic cell death in various cell types. However, the effect of TRPV1 on testicular apoptosis in aged mice has not yet been reported. TRPV1 knockout (KO) mice had a longer lifespan than that of wild-type (WT) mice. Lifespan was increased by 11.8% in male TRPV1 KO mice compared to that in WT mice. TRPV1 KO males lived approximately 100 days longer than WT males on average, and the maximum lifespan was markedly extended in TRPV1 KO mice compared with that in WT mice. The TRPV1 expression levels were highly increased in the testes of older mice. TRPV1 was expressed in the entire testes region of the old mice. In addition, old TRPV1 KO mice had lower testicular apoptosis than that of WT mice. Our results show that TRPV1 induces testicular apoptosis and suggest that TRPV1 may be associated with testicular aging.

TRPV1 activation induces cell death of TM3 mouse Leydig cells

“…However, another report showed that TRPV1 KO mice have an extended lifespan and maintained youthfulness compared to WT at the same age (Riera et al, 2014). Our recent study showed that TRPV1 could induce testicular apoptosis (Siregar et al, 2019). The TRPV1 expression levels are significantly higher in the testes of older mice, with a distinct age-dependent expression pattern: a predominant expression in the Leydig cells of young adult mice and a similar level of expression throughout the testes of older mice (Siregar et al, 2019).…”

“…TRPV1 KO mice have a testis phenotype with more cell death induced by oxidative stress stimuli and massive loss of germ cells from seminiferous tubules than wild-type (WT) mice Martins et al, 2014). However, another report showed that TRPV1 KO mice have an extended lifespan and maintained youthfulness compared to WT at the same age (Riera et al, 2014). Our recent study showed that TRPV1 could induce testicular apoptosis (Siregar et al, 2019).…”

Aquaporin 4 expression is downregulated in large bovine ovarian follicles

“…The experimental materials and methods were used similarly to previous studies conducted in this laboratory (Siregar et al, 2019;Choe et al, 2010).…”

“…The ovarian sections were used for immunostaining to identify the expression and localization of AQP4. The immunostaining procedures were performed as previously described (Siregar et al, 2019). Briefly, deparaffinized tissue sections were permeabilized with 0.2% Triton X-100 for 10 min at room temperature.…”

“…The quantified cDNA was used as a template. The RT-PCR procedures were performed as previously described (Siregar et al, 2019). The PCR steps included initial denaturation at 94℃ for 5 min, then 32 cycles at 94℃ for 20 sec, 55℃ for 20 sec, 72℃ for 20 sec, and a final extension step at 72℃ for 10 min.…”