Aquaporin 4 expression is downregulated in large bovine ovarian follicles

Kim, Chang-Woon; Choi, Eunju; Kim, Eun Jin; Siregar, Adrian S.; Han, Jaehee; Kang, Dawon

doi:10.12750/jarb.35.4.315

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…AQP4 might collaborate with TASK-3 for water and ion transport in GCs [32]. However, given the reduced levels of AQP4 in LFGCs [33], there may be limitations in water and ion transport.…”

Section: Discussionmentioning

confidence: 99%

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Downregulation of TASK-3 Channel Induces Senescence of Granulosa Cells in the Bovine Follicular Cystic Ovary

Kim,

Woo

et al. 2024

Preprint

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Ovarian cysts are linked to hormone imbalances and altered gene expressions, but the connection between cysts and ion channel expression is understudied. This study explored the role of TWIK-related acid-sensitive K+ (TASK) channels in bovine ovarian cyst formation. The ovarian follicles were split into small (5 to 10 mm in diameter) and large (> 25 mm in diameter) groups. Among the measured K+, Na+, and Cl- concentrations in follicular fluid (FF) obtained from small-sized follicles (SF) and large-sized follicles (LF), the K+ concentration was significantly lower in LFFF. Quantitative PCR, western blot, and immunocytochemistry data revealed that TASK-3 expression levels significantly decreased by approximately 50% in both LF and granulosa cells obtained from LF (LFGCs) compared to the corresponding controls. The TASK-3 protein was localized to the plasma membrane of GCs. The diameter of LFGCs was larger than that of SFGC. The cell swelling response to exposure to a hypotonic solution (200 mOsm/L) was highly reduced in TASK-3-overexpressing cells compared to vector-transfected cells. TASK-3-knockdown cells showed arrested growth. Senescence markers were detected in LFGCs and TASK-3-knockdown cells. These findings suggest that reduced TASK-3 expression in LF is associated with the inhibition of GC growth, leading to senescence and cyst formation.

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“…AQP4 might collaborate with TASK-3 for water and ion transport in GCs [32]. However, given the reduced levels of AQP4 in LFGCs [33], there may be limitations in water and ion transport.…”

Section: Discussionmentioning

confidence: 99%

“…The isolation of FF and GCs was performed as previously described [33]. Briefly, FF was carefully aspirated in follicles with a 10 mL syringe fitted with an 18-or 23-gauge needle.…”

Section: Isolation Of Follicular Fluid and Granulosa Cellsmentioning

confidence: 99%

Downregulation of TASK-3 Channel Induces Senescence of Granulosa Cells in the Bovine Follicular Cystic Ovary

Kim,

Woo

et al. 2024

Preprint

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“…Folliculogenesis can be divided into two rather distinct stages: the gonadotropin-independent (preantral) around 300 days and gonadotropin-dependent (antral or Graafian) periods for 50 days, respectively. After antrum formation, the follicle becomes dependent on FSH stimulation for continued growth and development (Giudice, 2001;Kim et al, 2020). Although FSH is the primary regulator of DF development, it is now clear that growth factors (GFs) produced by the follicle itself can act by autocrine and paracrine mechanisms to modulate, either amplify or attenuate, FSH action (Erickson and Shimasaki, 2001).…”

Section: Papp-a Related To Folliculogenesismentioning

confidence: 99%

Various expression patterns of pregnancy-associated plasma protein-A

Jeon

Lee

Son

et al. 2022

J Anim Reprod Biotechnol

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The zinc metalloproteinase, PAPP-A was firstly identified as one of the four placental antigens of unknown function found in high concentrations in the plasma of pregnant women (Gall and Halbert, 1972), hence the name 'pregnancy-associated plasma protein-A' (Lin et al., 1974). Subsequent studies found PAPP-A mRNA to be expressed in a variety of cell types and tissues unrelated to pregnancy (Overgaard et al., 1999).Along with PAPP-A, three other (non-proteolytic) pregnancy-associated proteins were described and were named PAPP-B, -C, and -D based on positions of precipitate lines in immunodiffusion experiments. PAPP-A was shown to enhance insulin-like growth factor (IGF) action through specific cleavage of inhibitory IGF binding protein (IGFBP), primarily IGFBP-4 (Lawrence et al., 1999). Cleavage of IGFBP-4 results in loss of binding affinity for IGF, causing increased IGF bioavailability for IGF-IRmediated proliferation, survival and migration (Conover and Oxvig, 2018).

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Gene Expression of Aquaporins (AQPs) in Cumulus Oocytes Complex and Embryo of Cattle

Petano-Duque

Castro-Vargas

Cruz-Méndez

et al. 2022

Animals

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Aquaporins (AQPs) are proteins with various functions related to proper cell function and early development in mammals. The aim of this study was to evaluate the presence of AQPs and determine their mRNA levels in the cumulus oocyte complex (COC) of four bovine breeds and in blastocysts of five bovine crosses. Grade I, II and III COCs were collected by ovum pick up from non-lactating heifers of the Brahaman, Holstein, Gir and Romosinuano breeds. Embryos were produced in vitro up to the blastocyst stage of the bovine ♀Gir × ♂Holstein, ♀Holstein × ♂Gir, ♀Brahman × ♂Holstein, ♀Holstein × ♂Brahman, and ♀Romosinuano × ♂Holstein crosses. mRNA expression of AQP1-AQP12b was estimated in COC and embryos by real-time-PCR. The presence of the twelve AQPs in the COCs and bovine embryos was established. Additionally, significant differences were determined in the expression of AQP6 and AQP12b in COCs, as well as in transcripts levels of AQP4, AQP8 and AQP9 from bovine embryos. Gene expression of AQPs in COCs and bovine embryos is consistent with the previously described biological functions. This is the first report of AQPs in COC of Gir, Brahman, Holstein and Romosinuano and embryos of five crossbreeds between Bos indicus and B. taurus.

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Aquaporin 4 expression is downregulated in large bovine ovarian follicles

Cited by 3 publications

References 24 publications

Downregulation of TASK-3 Channel Induces Senescence of Granulosa Cells in the Bovine Follicular Cystic Ovary

Downregulation of TASK-3 Channel Induces Senescence of Granulosa Cells in the Bovine Follicular Cystic Ovary

Various expression patterns of pregnancy-associated plasma protein-A

Gene Expression of Aquaporins (AQPs) in Cumulus Oocytes Complex and Embryo of Cattle

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