TRPV1 inactivation alters core body temperature and serum corticosterone levels: Impacts on clock genes expression in the liver and adrenal glands

Horta, Nayara AC.; Fernandes, Paola; Cardoso, Thais SR.; Machado, Frederico SM.; Drummond, Lucas Rios; Coimbra, Cândido Celso; Wanner, Samuel Penna; Castrucci, Ana Maria L.; Poletini, Maristela O.

doi:10.1016/j.jtherbio.2023.103514

Cited by 1 publication

(1 citation statement)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The study of circadian rhythms is necessary, as these rhythms play a vital role in preparing an organism's body for expected changes in the environment within a 24 h period. It is important for controlling various physiological processes, including the regulation of sleep, metabolism, stress response, blood pressure, body temperature, and the immune system [64,65]. The ontogeny of the coordinated entrainment of circadian rhythms and the HPA axis is an emergent investigational area [66,67].…”

Section: Discussionmentioning

confidence: 99%

Differential Expression of Circadian Clock Genes in the Bovine Neuroendocrine Adrenal System

Earnhardt-San,

Baker,

Riley

et al. 2023

Genes

View full text Add to dashboard Cite

Knowledge of circadian rhythm clock gene expression outside the suprachiasmatic nucleus is increasing. The purpose of this study was to determine whether expression of circadian clock genes differed within or among the bovine stress axis tissues (e.g., amygdala, hypothalamus, pituitary, adrenal cortex, and adrenal medulla). Tissues were obtained at an abattoir from eight mature nonpregnant Brahman cows that had been maintained in the same pasture and nutritional conditions. Sample tissues were stored in RNase-free sterile cryovials at −80 °C until the total RNA was extracted, quantified, assessed, and sequenced (NovaSeq 6000 system; paired-end 150 bp cycles). The trimmed reads were then mapped to a Bos taurus (B. taurus) reference genome (Umd3.1). Further analysis used the edgeR package. Raw gene count tables were read into RStudio, and low-expression genes were filtered out using the criteria of three minimum reads per gene in at least five samples. Normalization factors were then calculated using the trimmed mean of M values method to produce normalized gene counts within each sample tissue. The normalized gene counts important for a circadian rhythm were analyzed within and between each tissue of the stress axis using the GLM and CORR procedures of the Statistical Analysis System (SAS). The relative expression profiles of circadian clock genes differed (p < 0.01) within each tissue, with neuronal PAS domain protein 2 (NPAS2) having greater expression in the amygdala (p < 0.01) and period circadian regulator (PER1) having greater expression in all other tissues (p < 0.01). The expression among tissues also differed (p < 0.01) for individual circadian clock genes, with circadian locomotor output cycles protein kaput (CLOCK) expression being greater within the adrenal tissues and nuclear receptor subfamily 1 group D member 1 (NR1D1) expression being greater within the other tissues (p < 0.01). Overall, the results indicate that within each tissue, the various circadian clock genes were differentially expressed, in addition to being differentially expressed among the stress tissues of mature Brahman cows. Future use of these findings may assist in improving livestock husbandry and welfare by understanding interactions of the environment, stress responsiveness, and peripheral circadian rhythms.

show abstract