TRP-2 specifically decreases WM35 cell sensitivity to oxidative stress

Michard, Quentin; Commo, S; Belaïdi, Jean-Philippe; Alleaume, Anne-Marie; Michelet, Jean‐François; Daronnat, Edwige; Eilstein, Joan; Duché, Daniel; Marrot, Laurent; Bernard, Bruno

doi:10.1016/j.freeradbiomed.2007.11.021

Cited by 42 publications

(40 citation statements)

References 31 publications

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“…Because ferrocifenyl derivatives may be good candidates for melanoma cancer therapy and TRP-2 melanogenic protein has been involved in cell detoxification processes against particular chemotherapeutic agents as well as oxidative stress [26,32,33,40,41], we next explored the toxic potential of Fc-OHTam and Fc-diOH towards TRP-2 overexpressing WM35 melanoma cell line. Interestingly, TRP-2 overexpression did not provide any resistance to tested compounds suggesting they might have a broad toxic spectrum with respect to melanomas.…”

Section: Discussionmentioning

confidence: 99%

“…WM35, WM9, as well as the TRP-2 overexpressing WM35 derived clone WM35-C2 [26] were cultured in RPMI 1640 medium (GIBCO, Bethesda, MD, USA) containing 5% fetal bovine serum (FBS), 1% L-glutamine and 0.5% penicillin-streptomycin (GIBCO, Bethesda, MD, USA). WM793 cells were cultured in MCDB 153 supplemented with 20% L-15 medium (GIBCO, Bethesda, MD, USA), 2% FBS, 5 lg/ml insulin and 1.68 mM CaCl 2 .…”

Section: Cell Culturementioning

confidence: 99%

“…In particular, overexpression of the protein in the melanoma cell line WM35 has been shown to be beneficial to cell management of oxidative stress generated by hydrogen peroxide or paraquat [26]. Here we submitted the TRP-2 overexpressing clone WM35-C2 (Fig.…”

Section: Effect Of Ferrocenyl Derivatives In the Presence Of Trp-2mentioning

confidence: 99%

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Evaluation of cytotoxic properties of organometallic ferrocifens on melanocytes, primary and metastatic melanoma cell lines

Michard¹,

Jaouen²,

Vessières³

et al. 2008

Journal of Inorganic Biochemistry

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Section: Discussionmentioning

confidence: 99%

Section: Cell Culturementioning

confidence: 99%

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Evaluation of cytotoxic properties of organometallic ferrocifens on melanocytes, primary and metastatic melanoma cell lines

Michard¹,

Jaouen²,

Vessières³

et al. 2008

Journal of Inorganic Biochemistry

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“…The TRP2 protects human against oxidative stress by increasing glutathione levels and by reducing the toxicity of quinones and DNA damage induced by free radicals [58]. Several melanosome glycoproteins have been shown to be antigenic in human.…”

Section: Tyrosine Related Protein (Trp-2)mentioning

confidence: 99%

Autoantibodies Profile in Vitiligo

Hamid¹,

Bitsue²,

Mirshafiey³

2015

Pigmentary Disorders

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Vitiligo is one of the disease which is yet to understand its pathogenesis, however many studies associate this disease as an autoimmune. Detection of autoimmune cells in the serum, lesional and perilesional area of vitiligo patients gives more insight on the disease mechanism. Presence of autoantibodies against melanocytes antigens in vitiligo patients indicates an autoimmune involvement in the aetiology of the disease. Identification and characterization of vitiligo autoantibodies would pave the way for developing new laboratory test for diagnosis. Studying the autoantibodies profile can give an impression on the disease condition of vitiligo patients. We realized the need of research emphasis in this area as more is yet to be discovered. In this review we give an account on different autoantibodies and their associated autoantigens in vitiligo as another effort of providing an updated data for detail analysis.

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“…The DCT-melanocyte protection via its enzymatic products also functions for the benefit of pigmented malignant melanoma. A DCT-mediated pathway that enables melanoma cells with more resistance to chemo-/radiotherapeutic or oxidative stress (5)(6)(7)(8) has been reported. In a speculative proposed mechanism, the most stress-resistant pigmented melanoma cell lines, overexpressing endogenous DCT, will produce high amounts of the stable antioxidant DHICA, which may indirectly activate the ERK pathway (9).…”

mentioning

confidence: 99%

Cross-talk between Dopachrome Tautomerase and Caveolin-1 Is Melanoma Cell Phenotype-specific and Potentially Involved in Tumor Progression

Popa

Milac

Sima

et al. 2016

Journal of Biological Chemistry

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L-Dopachrome tautomerase (L-DCT), also called tyrosinaserelated protein-2 (TRP-2), is a melanoma antigen overexpressed in most chemo-/radiotherapeutic stress-resistant tumor clones, and caveolin-1 (CAV1) is a main regulator of numerous signaling processes. A structural and functional relationship between DCT and CAV1 is first presented here in two human amelanotic melanoma cell lines, derived from vertical growth phase (MelJuSo) and metastatic (SKMel28) melanomas. DCT co-localizes at the plasma membrane with CAV1 and Cavin-1, another molecular marker for caveolae in both cell phenotypes. Our novel structural model proposed for the DCT-CAV1 complex, in addition to co-immunoprecipitation and mass spectrometry data, indicates a possible direct interaction between DCT and CAV1. The CAV1 control on DCT gene expression, DCT posttranslational processing, and subcellular distribution is cell phenotype-dependent. DCT is a modulator of CAV1 stability and supramolecular assembly in both cell phenotypes. During autocrine stimulation, the expressions of DCT and CAV1 are oppositely regulated; DCT increases while CAV1 decreases. Sub-confluent MelJuSo clones DCT high /CAV1 low are proliferating and acquire fibroblast-like morphology, forming massive, confluent clusters as demonstrated by immunofluorescent staining and TissueFAXS quantitative image cytometry analysis. CAV1 down-regulation directly contributes to the expansion of MelJuSo DCT high subtype. CAV1 involved in the perpetuation of cell phenotype-overexpressing anti-stress DCT molecule supports the concept that CAV1 functions as a tumor suppressor in early stages of melanoma. DCT is a regulator of the CAV1-associated structures and is possibly a new molecular player in CAV1-mediated processes in melanoma.Cutaneous malignant melanoma remains the deadliest form of skin cancer worldwide (1). The skin melanocytes, normally located at the epidermis-dermis junction, start an uncontrolled proliferation process (radial growth phase, RGP), followed by dermis invasion (vertical growth phase, VGP).3 In a more advanced stage, melanoma cells migrate from this primary site to distant organs (liver, lung), populating them (metastasis) and eventually causing death (2). The mechanisms that specifically operate in tumor cell phenotypes determining the switch from the non-metastatic to metastatic stage are not entirely elucidated.Tyrosinase-related protein-2 (TRP-2 or Tyrp2) is a member of the tyrosinase-related protein family, together with tyrosinase (TYR) and tyrosinase-related protein-1 (TRP-1). Tyrosinase-related proteins are expressed by both melanocytes and melanoma forming the enzymatic cluster responsible for pigment production (3).TRP-2, also named dopachrome tautomerase (DCT), functions downstream of TYR by converting dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA), the precursor of eumelanins with a potent hydroxyl radical-scavenging activity (4). In the absence of DCT, dopachrome is spontaneously converted into 5,6-dihydroxyindole, a more toxic intermediate than...

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TRP-2 specifically decreases WM35 cell sensitivity to oxidative stress

Cited by 42 publications

References 31 publications

Evaluation of cytotoxic properties of organometallic ferrocifens on melanocytes, primary and metastatic melanoma cell lines

Evaluation of cytotoxic properties of organometallic ferrocifens on melanocytes, primary and metastatic melanoma cell lines

Autoantibodies Profile in Vitiligo

Cross-talk between Dopachrome Tautomerase and Caveolin-1 Is Melanoma Cell Phenotype-specific and Potentially Involved in Tumor Progression

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