Tropomyosins in mosquito and house dust mite cross‐react at the humoral and cellular level

Cantillo, José Fernando; Puerta, Leonardo; Fernández-Caldas, Enrique; Subiza, José Luis; Soria, Irene; Wöhrl, Stefan; Ebner, Christof; Keller, Walter; Resch, Yvonne; Vrtala, Susanne; Bohle, Barbara

doi:10.1111/cea.13229

Cited by 15 publications

(14 citation statements)

References 23 publications

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“…Compelling experimental evidence demonstrated the cross-reactivity between Der p 10 and Aed a 10.01 allergens at both humoral and cellular levels. 47 These findings highlight the presence of significant cross-reactivity between mosquitos and other arthropods, and this may be explained by tropomyosin.…”

Section: Tropomyosin and Mosquitoesmentioning

confidence: 88%

Tropomyosin: A panallergen that causes a worldwide allergic problem

Papia

Bellia

Uasuf

2021

allergy asthma proc

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Background: Panallergens are proteins that take part in key processes of organisms and, therefore, are ubiquitously distributed with highly conserved sequences and structures. One class of these panallergens is composed of the tropomyosins. The highly heat-stable tropomyosins comprise the major allergens in crustaceans and mollusks, which make them important food allergens in exposed populations. Tropomyosins are responsible for a widespread immunoglobulin E cross-reactivity among allergens from different sources. Allergic tropomyosins are expressed in many species, including parasites and insects. Methods: This panallergen class is divided, according to it capacity of induced allergic symptoms, into allergenic or nonallergenic tropomyosin. Although vertebrate tropomyosins share ∼55% of sequence homology with invertebrate tropomyosins, it has been thought that the invertebrate tropomyosins would not have allergic properties. Nevertheless, in recent years, this opinion has been changed. In particular, tropomyosin has been recognized as a major allergen in many insects. Results: A high grade of homology has been shown among tropomyosins from different species, such as crustaceans and insects, which supports the hypothesis of cross-reactivity among tropomyosins from divergent species. Moreover, the emerging habit of consuming edible insects has drawn the attention of allergists to invertebrate tropomyosin protein due to its potential allergenic risk. Nevertheless, evidence about tropomyosin involvement in clinical allergic response is still scarce and deserves more investigation. Conclusion: This review intended to report allergic reactions associated with different tropomyosins when considering house dust mites, parasites, seafood, and insects, and to summarize our current knowledge about its cross-reactivity because this could help physicians to accurately diagnose patients with food allergy.

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Section: Tropomyosin and Mosquitoesmentioning

confidence: 88%

Tropomyosin: A panallergen that causes a worldwide allergic problem

Papia

Bellia

Uasuf

2021

allergy asthma proc

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“…Commercially available skin prick tests and IgE ELISA are prepared using mosquito whole body extracts, which contain minute amounts of the relevant salivary allergens. In addition, they contain nonsalivary gland allergens, such as tropomyosin, that could be cross-reactive to other species such as house dust mite 21 or Cte f 2 that could crossreact with cat flea. 22 Moreover, the commercial test is based on A. communis which is not distributed in tropical areas.…”

Section: Discussionmentioning

confidence: 99%

Novel salivary gland allergens from tropical mosquito species and IgE reactivity in allergic patients

Opasawatchai

Yolwong

Thuncharoen

et al. 2020

World Allergy Organization Journal

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Background: Mosquito allergy is common in tropical countries but remains under-diagnosed.This may be due to the lack of knowledge and diagnostic tools for tropical mosquito allergens.Objective: We aimed to characterize allergens from tropical mosquito species and investigate IgE reactivity in mosquito-allergic patients to the salivary gland proteins from these mosquitoes.Methods: Salivary gland extract (SGE) from 4 mosquito species, highly distributed in the tropics, including Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles dirus b, were studied. SGE-specific IgE and IgG ELISA were developed, and serum from 64 mosquito-allergic and 22 non-allergic healthy control subjects was assayed. Further investigations using IgEimmunoblots followed by mass spectrometry analysis were performed to identify and characterize allergens from each species.Results: Mosquito-allergic subjects have detectable serum IgE to SGE derived from local mosquito species, while the IgE levels to Aedes communis using commercially available ELISA were mostly minimal. IgE-immunoblot analysis and mass spectrometry identified 5 novel mosquito allergens from A. albopictus (Aed al 2, Aed al 3), C. quinquefasciatus (Cul q 2.01, Cul q 3), and A. dirus b (Ano d 2). Interestingly, 4 of the 5 new allergens belong to the D7 protein family.Conclusions & clinical relevance: Five novel allergens from 3 tropical mosquito species were characterized. The majority of mosquito-allergic subjects who live in the tropics have IgE reactivity to these allergens. Our study paves the way for the development of diagnostic tests, componentresolved diagnostics, and future immunotherapy for mosquito allergy in tropical countries.

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“…PBMCs isolated from non-allergic donors (5 × 10^5 cells) were stripped in 2 mL ice cold lactic acid buffer (0.13 M KCl, 0.05 M NaCl, 0.01 M lactic acid, pH = 3.9) for 30 s as described before [18]. After washing 3 times by PBS, cells were pre-incubated with sera from HDMallergic individuals for 1 h at 37 °C.…”

Section: Basophil Activation Testmentioning

confidence: 99%

Tropomyosin in mugwort cross-reacts to house dust mite, eliciting non-Th2 response in allergic rhinitis patients sensitized to house dust mite

et al. 2021

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Background Mugwort and house dust mite (HDM) are two of the most common inhalant allergens in Asia, however, whether mugwort affects polysensitized HDM+ allergic rhinitis (AR) patients has not been elucidated. Methods Overall, 15,884 AR outpatients were assessed for clinical status. Amino acid sequences of mugwort were determined by mass spectrometry. Afterward, cross-reactivity between mugwort tropomyosin and Dermatophagoides pteronyssinus 10 (Der p10) was analysed by ELISA inhibition and basophil activation experiments. To compare immunologic responses eliciting by two different tropomyosins, peripheral blood mononuclear cells (PBMCs) of HDM-monosensitized patients were stimulated by mugwort, HDM, Der p10 and synthetic peptides representing mugwort tropomyosin respectively. Results Polysensitized HDM+AR patients were mainly sensitized to cat and mugwort, and the positive rate of monosensitized HDM+AR out-clinic patients was increased during the mugwort pollen season. Tropomyosin protein was able to find in mugwort. Synthetic tropomyosin peptide of mugwort activated basophils which were primed by HDM-specific IgE; ELISA inhibition experiment showed synthetic tropomyosin peptide of mugwort inhibited IgE binding to HDM tropomyosin, Der p10. Unlike HDM and Derp 10, mugwort and mugwort tropomyosin mainly induced IFN-γ and IL-17 release in PBMCs of monosensitized HDM+AR patients, but not IL-5. Conclusions Pan-allergen tropomyosin accounts for the cross-reactivity between mugwort and HDM, which reminds HDM+ patients to reduce mugwort exposure in mugwort pollen season in virtue of the tropomyosin induced mild inflammation.

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Tropomyosins in mosquito and house dust mite cross‐react at the humoral and cellular level

Abstract: Tropomyosins from D. pteronyssinus and A. aegypti show humoral and cellular cross-reactivity, involving 5 potential T cell-activating regions. The more pronounced cross-reactivity of Aed a 10.01 and Der p 10 matched the higher sequence similarity of both proteins.

Cited by 15 publications

References 23 publications

Tropomyosin: A panallergen that causes a worldwide allergic problem

Tropomyosin: A panallergen that causes a worldwide allergic problem

Novel salivary gland allergens from tropical mosquito species and IgE reactivity in allergic patients

Tropomyosin in mugwort cross-reacts to house dust mite, eliciting non-Th2 response in allergic rhinitis patients sensitized to house dust mite

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