Trophoblastic microRNAs are downregulated in a diabetic pregnancy through an inhibition of Drosha

Pendzialek, S. Mareike; Knelangen, Julia M.; Schindler, Maria; Gürke, Jacqueline; Grybel, Katarzyna J.; Gócza, Elen; Fischer, Bernd; Santos, Anne Navarrete

doi:10.1016/j.mce.2018.11.002

Cited by 5 publications

(3 citation statements)

References 86 publications

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“…The cells were lysed in RIPA buffer and protein concentration was measured using Bradford’s method. Nuclear proteins were isolated as previously described [ 18 ]. Briefly, the residual pallet was solubilized in 100 µL NLB buffer (50 mM Tris (pH 8.1), 10 mM ETDA, 1% SDS).…”

Section: Methodsmentioning

confidence: 99%

Nrf2/Keap1-Pathway Activation and Reduced Susceptibility to Chemotherapy Treatment by Acidification in Esophageal Adenocarcinoma Cells

Storz

Walther

Chemnitzer

et al. 2021

Cancers

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Chronic acid reflux causes cellular damage and inflammation in the lower esophagus. Due to these irritating insults, the squamous epithelium is replaced by metaplastic epithelium, which is a risk factor for the development of esophageal adenocarcinoma (EAC). In this study, we investigated the acid susceptibility in a Barrett’s cell culture in vitro model, using six cell lines, derived from squamous epithelium (EPC1 and EPC2), metaplasia (CP-A), dysplasia (CP-B), and EAC (OE33 and OE19) cells. Cells exposed to acidic pH showed a decreased viability dependent on time, pH, and progression status in the Barrett’s sequence, with the highest acid susceptibility in the squamous epithelium (EPC1 and EPC2), and the lowest in EAC cells. Acid pulsing was accompanied with an activation of the Nrf2/Keap1- and the NFκB-pathway, resulting in an increased expression of HO1—independent of the cellular context. OE33 showed a decreased responsiveness towards 5-FU, when the cells were grown in acidic conditions (pH 6 and pH 5.5). Our findings suggest a strong damage of squamous epithelium by gastroesophageal reflux, while Barrett’s dysplasia and EAC cells apparently exert acid-protective features, which lead to a cellular resistance against acid reflux.

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Section: Methodsmentioning

confidence: 99%

Nrf2/Keap1-Pathway Activation and Reduced Susceptibility to Chemotherapy Treatment by Acidification in Esophageal Adenocarcinoma Cells

Storz

Walther

Chemnitzer

et al. 2021

Cancers

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“…. Diabetes induced downregulation of DROSHA and DICER protein expression 97 , 98 . Drosha and Dicer expression is inhibited by high glucose treatment of primary skeletal muscle microvascular endothelial cells in mice 99 .…”

Section: Peripheral Exosome Synthesis and Central Exosome Absorption ...mentioning

confidence: 99%

Peripheral origin exosomal microRNAs aggravate glymphatic system dysfunction in diabetic cognitive impairment

Zhang

et al. 2023

Acta Pharmaceutica Sinica B

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“…Embryonic proteins and tissue samples were isolated as previously described [33,34]. Tissues were homogenized in 500 µL of cold radio immunoprecipitation assay (RIPA) buffer with protease and phosphatase inhibitor (Roche, Basel, Switzerland).…”

Section: Protein Preparation and Immunoblottingmentioning

confidence: 99%

Rabbit as an Aging Model in Reproduction: Advanced Maternal Age Alters GLO1 Expression in the Endometrium at the Time of Implantation

et al. 2020

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Advanced maternal age is associated with adverse pregnancy outcomes and the decline of female fertility in mammals. A potential reason for reduced fertility is metabolic changes due to protein modifications by advanced glycation end products. To elucidate the aging process in female reproduction, we analysed a key enzyme for detoxification of reactive dicarbonyls, the glyoxalase 1 (GLO1), in reproductive organs and blastocysts of young and old rabbits at the preimplantation stage. At day 6 post coitum, uterine, oviductal, ovarian tissue and blastocysts from young (16–20 weeks) and old rabbits (>108 weeks) were characterised for GLO1 expression. GLO1 amounts, enzymatic activity and localisation were quantified by qPCR, Simple Western, activity assay and immunohistochemistry. The GLO1 enzyme was present and active in all reproductive tract organs in a cell-type-specific pattern. Ovarian follicle and uterine epithelial cells expressed GLO1 to a high extent. In tertiary follicles, GLO1 expression increased, whereas it decreased in the endometrium of old rabbits at day 6 of pregnancy. In blastocysts of old animals, GLO1 expression remained unchanged. In early pregnancy, advanced maternal age leads to modified GLO1 expression in ovarian follicles and the endometrium, indicating an altered metabolic stress response at the preimplantation stage in older females.

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Trophoblastic microRNAs are downregulated in a diabetic pregnancy through an inhibition of Drosha

Cited by 5 publications

References 86 publications

Nrf2/Keap1-Pathway Activation and Reduced Susceptibility to Chemotherapy Treatment by Acidification in Esophageal Adenocarcinoma Cells

Nrf2/Keap1-Pathway Activation and Reduced Susceptibility to Chemotherapy Treatment by Acidification in Esophageal Adenocarcinoma Cells

Peripheral origin exosomal microRNAs aggravate glymphatic system dysfunction in diabetic cognitive impairment

Rabbit as an Aging Model in Reproduction: Advanced Maternal Age Alters GLO1 Expression in the Endometrium at the Time of Implantation

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