Trophectoderm regeneration to support full-term development in the inner cell mass isolated from bovine blastocyst

Kohri, Nanami; Akizawa, Hiroki; Iisaka, Sakie; Bai, Hanako; Yanagawa, Yuchio; Takahashi, Masashi; Komatsu, Masaya; Kawai, Masahito; Nagano, Masashi; Kawahara, Manabu

doi:10.1074/jbc.ra119.010746

Cited by 22 publications

(22 citation statements)

References 65 publications

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“…At day 7, we found a subset of embryos where SOX17 was not present yet, while in embryos that expressed the marker, intensity was low, mutually exclusive with NANOG and restricted to the ICM. Other studies using in vitro produced embryos report co-expression with NANOG as early as the 16-32 cell stage [10] and ectopic expression in the TE until day 6.5 [27]. In contrast to various reports using in vitro produced embryos [9,10,25], we did not find co-expression of GATA6 and NANOG in day 7 ex vivo embryos, indicating an earlier commitment to the HB or EPI lineage by reciprocal repression.…”

Section: Discussioncontrasting

confidence: 99%

The second lineage differentiation of bovine embryos fails in the absence of OCT4/POU5F1

Simmet¹,

Kurome²,

Zakhartchenko³

et al. 2021

Preprint

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The mammalian blastocyst undergoes two lineage segregations, i.e., formation of the trophectoderm and subsequently differentiation of the hypoblast (HB) from the inner cell mass, leaving the epiblast (EPI) the remaining pluripotent lineage. To clarify expression patterns of markers specific for these lineages in bovine embryos, we analyzed day 7, 9 and 12 blastocysts completely derived ex vivo by staining for OCT4, NANOG, SOX2 (EPI) and GATA6, SOX17 (HB) and identified genes specific for these developmental stages in a global transcriptomics approach. To study the role of OCT4, we generated OCT4-deficient (OCT4 KO) embryos via somatic cell nuclear transfer or in vitro fertilization. OCT4 KO embryos reached the expanded blastocyst stage by day 8 but lost of NANOG and SOX17 expression, while SOX2 and GATA6 were unaffected. Blastocysts transferred to recipient cows from day 6 to 9 expanded, but the OCT4 KO phenotype was not rescued by the uterine environment. Exposure of OCT4 KO embryos to exogenous FGF4 or chimeric complementation with OCT4 intact embryos did not restore NANOG or SOX17 in OCT4-deficient cells. Our data show, that OCT4 is required cell-autonomously for the maintenance of pluripotency of the EPI and differentiation of the HB in bovine embryos.

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Section: Discussioncontrasting

confidence: 99%

The second lineage differentiation of bovine embryos fails in the absence of OCT4/POU5F1

Simmet¹,

Kurome²,

Zakhartchenko³

et al. 2021

Preprint

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“…In the mouse, the ICM is unable to differentiate into TE (Nichols and Gardner, 1984), whereas human ICM cells from E6 blastocysts readily differentiate into this lineage (Guo et al, 2020). In cattle, isolated ICMs can reconstitute a blastocyst (Kohri et al, 2019), demonstrating that a fully competent TE can emerge from ICMs. In experiments using mouse embryonic stem cells (ESCs), the in vitro equivalent of the pre-implantation naive epiblast shows that these cells cannot differentiate to TE in chimeras (Beddington and Robertson, 1989;Bradley et al, 1984).…”

Section: Peri-implantation Development In Mammalian Embryos With Bilaminar Discsmentioning

confidence: 99%

Conserved features of non-primate bilaminar disc embryos and the germline

2021

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Post-implantation embryo development commences with a bilaminar disc in most mammals, including humans. Whereas access to early human embryos is limited and subject to greater ethical scrutiny, studies on non-primate embryos developing as bilaminar discs offer exceptional opportunities for advances in gastrulation, the germline, and the basis for evolutionary divergence applicable to human development. Here, we discuss the advantages of investigations in the pig embryo as an exemplar of development of a bilaminar disc embryo with relevance to early human development. Besides, the pig has the potential for the creation of humanized organs for xenotransplantation. Precise genetic engineering approaches, imaging, and single-cell analysis are cost effective and efficient, enabling research into some outstanding questions on human development and for developing authentic models of early human development with stem cells.

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“…This period of full ICM potency can be prolonged until the 64-to 128-cell stage in mouse when embryos are cultured in ERK inhibitors from the morula stage, suggesting that ERK activity is not only necessary for PrE formation, but it also plays a role in final TE and ICM specification (Wigger et al, 2017). A significantly extended period of ICM potency has been observed in non-murine mammals such as in cattle, where blastocysts regenerated from isolated ICMs and transferred to the recipient mother can result in live births (Kohri et al, 2019).…”

Section: Potency and Plasticity In Mammalian Developmentmentioning

confidence: 99%

“…2B) (Anani et al, 2014;Maître et al, 2016;Niwayama et al, 2019). As a result, the cells that inherit the apical domain are less contractile than their non-polar counterparts, Frum et al, 2013;Nichols et al, 1998;Palmieri et al, 1994Chambers et al, 2003Mitsui et al, 2003Avilion et al, 2003Wicklow et al, 2014Chazaud et al, 2006Schrode et al, 2014Artus et al, 2011Niakan et al, 2010 Dietrich andHiiragi, 2007;Strumpf et al, 2005 Kirchhof et al, 2000;van Eijk et al, 1999;Madeja et al, 2013Khan et al, 2012Kuijk et al, 2012Kuijk et al, , 2008Madeja et al, 2013 Goissis andCibelli, 2014a;Khan et al, 2012;Ozawa et al, 2012Khan et al, 2012Kuijk et al, 2012Kuijk et al, , 2008Madeja et al, 2013Canizo et al, 2019Kohri et al, 2019;Negrón-Pérez et al, 2017Berg et al, 2011Goissis and Cibelli, 2014b;…”

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confidence: 99%

Common principles of early mammalian embryo self-organisation

Płusa

Piliszek

2020

Development

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Pre-implantation mammalian development unites extreme plasticity with a robust outcome: the formation of a blastocyst, an organised multi-layered structure ready for implantation. The process of blastocyst formation is one of the best-known examples of self-organisation. The first three cell lineages in mammalian development specify and arrange themselves during the morphogenic process based on cell-cell interactions. Despite decades of research, the unifying principles driving early mammalian development are still not fully defined. Here, we discuss the role of physical forces, and molecular and cellular mechanisms, in driving self-organisation and lineage formation that are shared between eutherian mammals.

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Trophectoderm regeneration to support full-term development in the inner cell mass isolated from bovine blastocyst

Cited by 22 publications

References 65 publications

The second lineage differentiation of bovine embryos fails in the absence of OCT4/POU5F1

The second lineage differentiation of bovine embryos fails in the absence of OCT4/POU5F1

Conserved features of non-primate bilaminar disc embryos and the germline

Common principles of early mammalian embryo self-organisation

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