Triticum aestivum in open skin wounds: cytotoxicity and collagen histopathology

Abstract

Phytoterapic compounds have been used in wound healing for many centuries. Nowadays, scientific evidences of phytotherapeutics is a requirement of the legislation. The scientific literature notes the need for healing topics yielding scars that are both aesthetically appealing and resistant. We aimed to evaluate the cytotoxicity of several doses of T. aestivum extract (2 mg mL -1 , 4 mg mL -1 , 6 mg mL -1 , 8 mg mL -1 and 10 mg mL -1 ) in a fibroblast cell line and the healing process in an in vivo experimental model (New Zealand rabbits). For this, MTT test in 3T6 cells was performed in duplicates using MEM (0 mg ml -1 ) as negative control. Cell viability was calculated as: absorbance average in treatments/ absorbance average in controls x 100. In vivo test was performed in 78 skin wounds in rabbits that were treated with 2 mg ml -1 and 10 mg ml -1 of T. aestivum and non-ionic cream for 21 days. After this period, it was evaluated the histology using picrosorius and Gomori's trichrome staining. Statistical analysis was evaluated using T test (Graphpad) for cytotoxicity assay, Fischer test for the gomori trichrome test (Grahpad) and Kruskal-Wallis (Statistic 9.0) for picrosirius test. The in vitro test resulted in cytotoxicity observed at 2mg mL -1 whereas cells were viable at higher doses. On the other hand, it was observed that collagen formation of wounds was more uniform with this dose than with 10mg mL -1 extract in the in vivo study. Thus, we conclude that the 2mg mL -1 T. aestivum aqueous extract dose was more efficient in the in vivo wound healing study, despite its cytotoxic effects in vitro. Key words: Wound healing. Rabbits. Picrosirius. Gomori trichome. Wheat. ResumoOs extratos vegetais têm sido utilizados na cicatrização de feridas a muitos séculos. No entanto nos dias atuais a comprovação científica dos fitoterápicos é uma exigência da legislação. Na literatura científica se observa a necessidade de cicatrizantes tópicos que proporcionem uma cicatriz estética e resistente. Devido a isso objetivou-se avaliar a citotoxicidade de diversas doses de T. aestivum (2 mg mL 1 , 4 mg mL -1 , 6 mg mL -1 , 8 mg mL -1 e 10 mg mL -1 ) em linhagem celular de fibroblasto, e o processo cicatricial em modelo experimental (New Zealand rabbits) in vivo. Para isso foi realizado o teste de MTT em linhagem celular 3T. Tests were performed in duplicates, using MEM (0 mg mL -1 ) as negative control. Cell viability was calculated as: absorbance average in treatments/absorbance average

Antioxidant, α-Glucosidase Inhibitory, and Anti-Inflammatory Activities and Cell Toxicity of Waxy and Normal Wheat Sprouts at Various Germination Time

“…Therefore, the enhancement of viability or non-cytotoxicity on these cells is beneficial for human health. Wheat aqueous extract was reported to increase the cell viability with an increase in the dose of the extract [42], but there have been no reports on wheat sprout. Apigenin, a flavonoid in plants, and arabinoxylan, a soluble dietary fiber, are known to promote cell viability [43,44] and are present in wheat and wheat sprout [23,43,45].…”

Lesões cutâneas traumática em pequenos animais: características e tratamentos

“…Estudos em cicatrização comprovam a ação plantas medicinais populares como a babosa, calêndula, trigo, cajueiro, mastrunço e urucum, usadas para estimular a cicatrização. Elas apresentam diferentes potenciais terapêuticos, como por exemplo, a babosa que possui ação anti-inflamatória, o cajueiro e urucum estimulam a formação de tecido de granulação e o trigo que age na fibroplasia, assim é imprescindível o conhecimento das plantas medicinais e do tipo de lesão para que tenha uma cicatrização adequada e de qualidade (CAPELLA et al, 2016;PAVLETIC, 2018;PIRIZ et al, 2014;TILLMANN et al, 2018).…”