Triterpenoid Saponins from Leaves of Hedera pastuchowii

Abstract

Five new triterpenoid saponins, pastuchoside A (1), B (3), C (5), D (7) and E (9), were isolated from the leaves of Hedera pastuchowii. They have oleanolic acid or hederagenin as aglycone. The structures were established by NMR spectroscopy including gs (gradient selected)-COSY, gs-HSQC, gs-HSQC-TOCSY and gs-HMBC experiments, and mass spectrometry ( ESI-HR-MS). Heptaoside saponins, compounds 1 and 3, are described for the first time in the genus Hedera.

Evaluation of Antinociceptive and Anti-Inflammatory Activities of a New Triterpene Saponin from Bauhinia variegata Leaves

“…Normal control Saline 38.3 ± 0.8 --9 40 30.2 ± 1.9 -21.3* 38* 9 80 28.3 ± 2.7* -26.2* 47* 9 100 27.5 ± 2.4* -28.3* 51* 9 200 23.1 ± 1.8* -39.6* 71* Indomethacine 20 16.9 ± 1.6* -55.9* 100* * Signifi cant different from control group at P < 0.05. , corresponding to the loss of pentoside from the latter fragment. These data together with the alkaline hydrolysis was compatible with a structure of hydroxyolean 28-rhamnosyl-glucosyl-glucosyl ester with an O-rhamnosyl-pentoside, most probably at C-3 (Mshvildadze et al, 2004) (TLC and PC with the authentic samples). The resonances due to six sp 3 methyl carbon atoms at δ C 13.…”

“…00, 102.40, 95.29, 104.52, and 101.32, respectively (Table I). The sugar moieties were deduced to adopt α- and 13 C resonances were assigned by the aid of HSQC and HMBC correlation peaks and by comparison with the corresponding data of structurally related compounds (Mshvildadze et al, 2004;Shao et al, 1989).…”

Antioxidant, anti-inflammatory and anticancer activities of methanolic extracts from Ledum groenlandicum Retzius

“…The most active fraction (band 3) was separated by TLC analysis using chloroform:ethanol 20:1 as eluant and sulfuric acid 20% in methanol as developer. The pink coloring of the main compound in the fraction suggested a triterpene (Mshvildadze et al, 2004). To isolate this compound, the diethyl ether extract (6.62 g) was separated by silica gel column using as eluant chloroform (4 L), chloroform:methanol 90:1 (2.8 L) and chloroform:methanol 80:1 (2.8 L).…”

Évaluation de l'activité biologique du Ledum groenlandicum Retzius /

“…The most active fraction (band 3) was separated by TLC analysis using chloroform:ethanol 20:1 as eluant and sulfuric acid 20% in methanol as developer. The pink coloring of the main compound in the fraction suggested a triterpene (Mshvildadze et al, 2004). To isolate this compound, the diethyl ether extract (6.62 g) was separated by silica gel column using as eluant chloroform (4 L), chloroform:methanol 90:1 (2.7 L) and chloroform:methanol 80:1 (2.8 L).…”