Triterpene Saponins from Primula veris Subsp. macrocalyx and Primula elatior Subsp. meyeri

Oleanane-type triterpenes of Embelia schimperi leaves

“…This was further supported by the characteristic retro-Diels- (Hiradate et al, 1999;Sukumar et al, 1995). In fact, the relatively low field values for the oxymethine (d 4.65) and oxymethylene protons (d 4.30 and 3.76) in comparison with primulagenin A Calis et al, 1992;Sindambiwe et al, 1996), indicated that the acetyl groups were at C-3 and C-28, confirmed by HMBC experiments (Fig. 1) and further supported by the CIMS splitting pattern (Fig.…”

“…The spectrum also indicated the presence of two oxygen-bearing carbon methines showing resonances at d 80.0 (C-3) and 69.4 (C-16). The CIMS strong fragments at m/z 249 and 264 were attributable to the fragments arising from the cleavage of the 9-11 and 8-14 bonds and their formation further supported the presence of a hemiacetal function and a hydroxyl group in rings D/E, whereas the acetyl group was present in rings A/B (Calis et al, 1992). The site of esterification as C-3 was evident from the long-range C-H correlations between C-3 (d 80.0) and H-5 (d 0.80) as observed in the HMBC spectrum.…”

“…Additional significant signals observed at d 4.70 (1H, dd, J = 10.5, 5. 1 Hz,, 4.12 (1H, d, J = 3.5 Hz, H-16) and a typical singlet at d 4.80 (1H, s, H-28) suggested the presence of a 13, 28-oxydo moiety with a hemiacetal function at C-28 (Calis et al, 1992;Calis, 1989). The 13 C NMR further supported this: d 99.6 (H-C(O-)-OH) and 87.2 (-C(O-)-).…”

Saponins in tissue culture of Primula veris L.

“…Other triterpenoid saponins have been identified from the roots of Primula sp. in previous studies (Ahmad et al 1988;Çalis and Yürüker 1992;Ahmad and Shah 1993;Yayli 2001;Müller et al 2006). The fact that the PA I present in the medium can constitute up to 40% of the total PA I in some lines of root cultures puts the amount in root cultures in perspective.…”

Triterpene Saponins from Plants of the Flora of Turkey

“…macrocalyx and P. elatior subsp. meyeri (Calis et al, 1992). Primulasaponin (9) was isolated as the major saponin of P. auriculata, P. megaseifolia, P. longipes, and P. elatior subsp.…”