Triploidization of hybrids (female zebrafish × male blunt snout bream) by heat-shock can improve survival rate

Fu, Wen; Peng, Liangyue; Wu, Xianlong; He, Sheng; Zhao, Han; Liu, Jinhui; Liu, Wenbin; Xiao, Yamei

doi:10.1016/j.aquaculture.2019.734786

Cited by 6 publications

(3 citation statements)

References 34 publications

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“…Distant hybridization is an important technique for generating polyploidy, and it can lead to an altered genotype and phenotype of the offspring (Liu et al, 2016;Ren et al, 2019). Parsons et al (1986) found that 3n rainbow trout × coho salmon hybrids showed increased resistance to infectious hematopoietic necrosis virus, and Fu et al (2020) reported that the artificial induction of triploidy may improve the survival rate of distant hybrids. Xiao et al (2019) showed that 3n crucian carp displayed stronger disease resistance compared with their parents and proposed that this effect occurred through the mitochondrial antiviral-signaling protein.…”

Section: Discussionmentioning

confidence: 99%

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Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp

et al. 2021

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Previous research has indicated that triploid crucian carp (3n fish) have preferential resistance to cadmium (Cd) compared to Carassius auratas red var. (2n fish). In this article, comparative research is further conducted between the 2n and 3n fish in terms of the immune response to Cd-induced stress. Exposure to 9 mg/L Cd for 96 h changed the hepatic function indexes remarkably in the 2n fish, but not in the 3n fish. In the serum of Cd-treated 2n fish, the levels of alanine amino transferase, aspartate aminotransferase, adenosine deaminase, and total bilirubin significantly increased, while the levels of total protein, albumin, lysozyme, and anti-superoxide anion radicals decreased demonstrating hepatotoxicity. By analysis of transcriptome profiles, many immune-related pathways were found to be involved in the response of 3n fish to the Cd-induced stress. Expression levels of the immune genes, including the interleukin genes, tumor necrosis factor super family member genes, chemokine gene, toll-like receptor gene, and inflammatory marker cyclooxygenase 2 gene were significantly enhanced in the hepatopancreas of the Cd-treated 3n fish. In contrast, the expression levels of these genes decreased in the 2n fish. This research provides a theoretical basis for polyploid fish breeding and is helpful for the ecological restoration of water due to pollution.

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Section: Discussionmentioning

confidence: 99%

“…(2n fish) and Xiangyunji fish (3n fish) raised at the Engineering Center of Polyploidy Fish Breeding of the National Education Ministry located at Hunan Normal University were obtained for use in this study. The ploidy level of each sample was confirmed by a flow cytometer assay (Fu et al, 2020).…”

Section: Sample Preparationmentioning

confidence: 99%

Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp

et al. 2021

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“…Detailed heat shock treatment of fertilized eggs to induce triploid zebrafish can be referred to our previous report (Fu et al 2020). Briefly, the night before experiment, female and male parent zebrafish were separated and kept in the dark for 10 h. On the day of the experiment, parent zebrafish were first paired.…”

Section: Broodstock Management and Heat Shock Treatmentmentioning

confidence: 99%

Bisexual Fertile Triploid Zebrafish (Danio rerio): a Rare Case

Peng

et al. 2020

Mar Biotechnol

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Previous studies have suggested that artificially induced triploid zebrafish are exclusively male-biased. Owing to greatly inhibited gonadal development for the artificially induced triploid fish, they are regarded to be sterile in general. In this article, partially fertile bisexual triploid zebrafish are produced by suppressing extrusion of the second polar body by heat shock. Histological observation confirms that the early gonadal development of these triploid zebrafish is normal. Backcrossing and self-crossing are used to demonstrate that both the female and male triploid zebrafish have partial reproductive ability. Their dynamic of chromosomes during meiosis is revealed from the chromosome preparations of gonads. Examination of the expressed gonadal development-related genes shows some molecular evidence of the normal gonadal development in the triploid zebrafish. Clearly, these fertile bisexual triploid zebrafish can provide a unique system to study sex determination, as well as aneuploidy associated human diseases such as infertility and pregnancy loss.

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Identification of gynogenetic Megalobrama amblycephala induced by red crucian carp sperm and establishment of a new hypoxia tolerance strain

Chu

Xiao

et al. 2022

Aquaculture

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Triploidization of hybrids (female zebrafish × male blunt snout bream) by heat-shock can improve survival rate

Cited by 6 publications

References 34 publications

Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp

Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp

Bisexual Fertile Triploid Zebrafish (Danio rerio): a Rare Case

Identification of gynogenetic Megalobrama amblycephala induced by red crucian carp sperm and establishment of a new hypoxia tolerance strain

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