Triploid lion-paw scallop (Nodipecten subnodosus Sowerby); growth, gametogenesis, and gametic cell frequencies when grown at a high food availability site

Maldonado-Amparo, Rosalío; Ramı́rez, José Luis; Avila, Susana; Ibarra, Ana M.

doi:10.1016/j.aquaculture.2003.12.014

Cited by 44 publications

(30 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The frequently observed minimal fertility of triploid individuals is a general pattern, directly related to the ploidy level and already mentioned for numerous species in both plants and animals, which seems to be independent of other indirect effects. One causal factor of the infertility of triploid individuals is related to chromosomal pairing, segregation during meiosis and the fact that homologous chromosomes fail to synapse due to genomic imbalance , Crane & Sleeper 1989, Otto & Whitton 2000, Maldonado-Amparo et al 2004. This is consistent with the results of the present and some previous studies (Allen & Downing 1990, Normand et al 2008, all of which show that the difficulty met by triploid Pacific oysters in developing gonadic tissues occurs in the final stage of gametogenesis.…”

Section: Disturbance Of Gametogenesis In Triploid Oysterssupporting

confidence: 82%

Reproductive effort and growth in Crassostrea gigas: comparison of young diploid and triploid oysters issued from natural crosses or chemical induction

Normand¹,

Ernande²,

Haure³

et al. 2009

Aquat. Biol.

View full text Add to dashboard Cite

Early reproductive effort and growth were measured in 3 groups of the Pacific oyster Crassostrea gigas: 1 diploid group and 2 triploid groups resulting from either chemical induction (3nCB) or crosses between tetraploid and diploid parents (3nDT). Oysters were reared under intensive nursery conditions and sampled when 5 mo old. Reproductive effort was estimated by crosssectional area measurements of the visceral mass (i.e. gonadic occupation) and maturation stage was assessed by qualitative histology. As expected, comparison of the reproductive patterns of these 3 groups revealed a lower reproductive effort in triploid individuals relative to diploid. However, gonadic occupation in triploid oysters was higher than expected in both 3nCB and 3nDT groups, as the gonadic occupation was 47% of that in diploids at the sampling date. Our results suggest that, despite much lower mature gamete production in triploid oysters relative to diploid, their reproductive effort can be significant, even in young individuals. Additionally, a significant relationship was observed between gender and body mass within each group and for gonadic occupation in the diploid group, suggesting that there is a link between sex and fitness-related traits in C. gigas.

show abstract

Section: Disturbance Of Gametogenesis In Triploid Oysterssupporting

confidence: 82%

Reproductive effort and growth in Crassostrea gigas: comparison of young diploid and triploid oysters issued from natural crosses or chemical induction

Normand¹,

Ernande²,

Haure³

et al. 2009

Aquat. Biol.

View full text Add to dashboard Cite

show abstract

“…Maintaining the germline after the first maturation and spawn would be expected in the scallop because a second maturation will occur approximately one year after the first one (see [4], [11]). Furthermore, the sexual identity of male or female germ cells has to be either maintained or specified when a new gametogenic cycle begins.…”

Section: Discussionmentioning

confidence: 99%

“…These studies have shown that the triploid condition induced by inhibiting the release of the second polar body in fertilized eggs, and therefore the conclusion of meiosis II, induces not only functional sterility, but also structural sterility as practically no gametes of either sex are produced in adult triploids [11]. This is not the case for all triploid mollusks, but complete or structural sterility has been found for some triploid-induced species [12]–[14] whereas other species, including other scallops, are capable of producing gametes [15]–[17], even if they are aneuploids [18].…”

Section: Introductionmentioning

confidence: 99%

Identification of Male Gametogenesis Expressed Genes from the Scallop Nodipecten subnodosus by Suppressive Subtraction Hybridization and Pyrosequencing

et al. 2013

View full text Add to dashboard Cite

Despite the great advances in sequencing technologies, genomic and transcriptomic information for marine non-model species with ecological, evolutionary, and economical interest is still scarce. In this work we aimed to identify genes expressed during spermatogenesis in the functional hermaphrodite scallop Nodipecten subnodosus (Mollusca: Bivalvia: Pectinidae), with the purpose of obtaining a panel of genes that would allow for the study of differentially transcribed genes between diploid and triploid scallops in the context of meiotic arrest and reproductive sterility. Because our aim was to isolate genes involved in meiosis and other testis maturation-related processes, we generated suppressive subtractive hybridization libraries of testis vs. inactive gonad. We obtained 352 and 177 ESTs by clone sequencing, and using pyrosequencing (454-Roche) we maximized the identified ESTs to 34,276 reads. A total of 1,153 genes from the testis library had a blastx hit and GO annotation, including genes specific for meiosis, spermatogenesis, sex-differentiation, and transposable elements. Some of the identified meiosis genes function in chromosome pairing (scp2, scp3), recombination and DNA repair (dmc1, rad51, ccnb1ip1/hei10), and meiotic checkpoints (rad1, hormad1, dtl/cdt2). Gene expression analyses in different gametogenic stages in both sexual regions of the gonad of meiosis genes confirmed that the expression was specific or increased towards the maturing testis. Spermatogenesis genes included known testis-specific ones (kelch-10, shippo1, adad1), with some of these known to be associated to sterility. Sex differentiation genes included one of the most conserved genes at the bottom of the sex-determination cascade (dmrt1). Transcript from transposable elements, reverse transcriptase, and transposases in this library evidenced that transposition is an active process during spermatogenesis in N. subnodosus. In relation to the inactive library, we identified 833 transcripts with functional annotation related to activation of the transcription and translation machinery, as well as to germline control and maintenance.

show abstract

“…There are several examples of studies on triploidy induction on scallops, including species such as Argopecten irradians (Cogswell, Kenchington, Roach, & MacDonald, ; Tabarini, ; Zarnoch, Surier, Karney, Schreibman, & Gamss, ), A. purpuratus (Canello, Paredes, & Toro, ; Lohrmann & von Brand, ; Toro, Sanhueza, Paredes, & Canello, ; Winkler, Ladrón de Guevara, Estévez, & Jollán, ), A. ventricosus (Ruiz‐Verdugo, Allen, & Ibarra, ; Ruiz‐Verdugo, Ramírez, Allen, & Ibarra, ), Chlamys nobilis (Komaru, Uchimura, Ieyama, & Wada, ; Zeng et al., ), C. farreri (Yang, Zhang, & Guo, ), C. varia (Barón, Diter, & Bodoy, ), Nodipecten subnodosus (Maldonado‐Amparo, Ramírez, Ávila, & Ibarra, ), Patinopecten yessoensis (Meng et al., ; Yang, Li, & Guo, ), Pecten maximus (Beaumont, ), and Placopecten magellanicus (Desroisers et al., ). The efficiency of triploidy induction varies with different species and techniques.…”

Section: Introductionmentioning

confidence: 99%

Effect of three triploidy induction methods on the growth and survival of larvae and post-larvae of the Caribbean scallopArgopecten nucleus

2018

View full text Add to dashboard Cite

Argopecten nucleus is a small scallop from the Caribbean Sea and a relatively new species for aquaculture. One of the key challenges to develop the farming operations for this species from the current pilot scale to commercial level is to improve its harvest size. In this study, we tested three different methods for triploidy induction. Additionally, the effect of these protocols on survival, developmental rate and size of larvae and post‐larvae were assessed. Three different mechanisms to stimulate the inhibition of the release of the second polar body were tested; (1) cold shock (18°C); (2) 6‐dimethylaminopurine (6‐DMAP); (3) cytochalasin B (CB) and (4) dimethylsulphoxide (DMSO). The treatment with 6‐DMAP yielded the highest percentage of triploid larvae (39%). The survival and development rate, however, were higher in non‐treated larvae (control) than in the treatment groups. Interestingly, larvae from CB and the DMSO control groups exhibited lower growth rates in length than those from control and the other two treatments. No influence of the triploidy induction treatments was observed on post‐larvae survival, but the size of post‐larvae was larger for the cold shock treatment and DMSO control group. Our results indicate that the use of 6‐DMAP has the greatest potential to produce triploid larvae of A. nucleus without affecting negatively growth and survival of post‐larvae.

show abstract

Triploid lion-paw scallop (Nodipecten subnodosus Sowerby); growth, gametogenesis, and gametic cell frequencies when grown at a high food availability site

Cited by 44 publications

References 33 publications

Reproductive effort and growth in Crassostrea gigas: comparison of young diploid and triploid oysters issued from natural crosses or chemical induction

Reproductive effort and growth in Crassostrea gigas: comparison of young diploid and triploid oysters issued from natural crosses or chemical induction

Identification of Male Gametogenesis Expressed Genes from the Scallop Nodipecten subnodosus by Suppressive Subtraction Hybridization and Pyrosequencing

Effect of three triploidy induction methods on the growth and survival of larvae and post-larvae of the Caribbean scallopArgopecten nucleus

Contact Info

Product

Resources

About