Triple-Negative Breast Cancer Comparison With Canine Mammary Tumors From Light Microscopy to Molecular Pathology

Namagerdi, Asadoor; d’Angelo, Danila; Ciani, Francesca; Iannuzzi, Carmelina Antonella; Napolitano, Francesco; Avallone, Luigi; Laurentiis, Michelino De; Giordano, Antonio

doi:10.3389/fonc.2020.563779

Cited by 15 publications

(15 citation statements)

References 171 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, fragments removed from female dogs will not represent all existing tumor clones. On the contrary, since BC is smaller in women, the fragment achieves a greater representation of the tumor as a whole [2,25].…”

Section: Discussionmentioning

confidence: 96%

“…By recognizing the similarities of BC molecular background in human and canine species, we further evaluated 37 BC samples (biopsy of fragments and plasma) in dogs. The canine mammary tumor (CMT) has been proposed as a promising model resembling woman BC, since they share common components regarding the physiopathology of the disease [2,24]. We extracted DNA from these samples to construct a genomic library for NGS, ensuring the high quality of the procedures with respect to the efficacy and functionality of liquid biopsy.…”

Section: Discussionmentioning

confidence: 99%

“…Breast cancer (BC) in women and dogs shares similarities, such as high rate of recurrence and metastasis and a high mortality rate [1,2]. BC represents the second leading cause of cancer death in women worldwide [3].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Liquid Biopsy as a Diagnostic and Prognostic Tool for Women and Female Dogs with Breast Cancer

Colombo

Moschetta-Pinheiro

Novais

et al. 2021

Cancers

View full text Add to dashboard Cite

Introduction: Breast cancer (BC) is the malignant neoplasm with the highest mortality rate in women and female dogs are good models to study BC. Objective: We investigated the efficacy of liquid biopsy to detect gene mutations in the diagnosis and follow-up of women and female dogs with BC. Materials and Methods: In this study, 57 and 37 BC samples were collected from women and female dogs, respectively. After core biopsy and plasma samples were collected, the DNA and ctDNA of the tumor fragments and plasma were processed for next generation sequencing (NGS) assay. After preprocessing of the data, they were submitted to the Genome Analysis ToolKit (GATK). Results: In women, 1788 variants were identified in tumor fragments and 221 variants in plasma; 66 variants were simultaneously detected in tumors and plasma. Conversely, in female dogs, 1430 variants were found in plasma and 695 variants in tumor fragments; 59 variants were simultaneously identified in tumors and plasma. The most frequently mutated genes in the tumor fragments of women were USH2A, ATM, and IGF2R; in female dogs, they were USH2A, BRCA2, and RRM2. Plasma of women showed the most frequent genetic variations in the MAP3K1, BRCA1, and GRB7 genes, whereas plasma from female dogs had variations in the NF1, ERBB2, and KRT17 genes. Mutations in the AKT1, PIK3CA, and BRIP genes were associated with tumor recurrence, with a highly pathogenic variant in PIK3CA being particularly prominent. We also detected a gain-of-function mutation in the GRB7, MAP3K1, and MLH1 genes. Conclusion: Liquid biopsy is useful to identify specific genetic variations at the beginning of BC manifestation and may be accompanied over the entire follow-up period, thereby supporting the clinicians in refining interventions.

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Liquid Biopsy as a Diagnostic and Prognostic Tool for Women and Female Dogs with Breast Cancer

Colombo

Moschetta-Pinheiro

Novais

et al. 2021

Cancers

View full text Add to dashboard Cite

show abstract

“…The luminal subtype generally carries a good prognosis, and luminal B tumors have a much poorer prognosis than the luminal A subtype [33]. The basal subtype comprises ER-PR-HER2-(triple-negative) tumors with an expression profile that mimics basal epithelial cells from other parts of the body and normal breast myoepithelial cells [34]. This subtype has a low expression of hormone receptors and HER2 and high expression of basal markers and proliferation-associated genes.…”

Section: Molecular Development Of Breast Cancermentioning

confidence: 99%

α-Mangostin Nanoparticles Cytotoxicity and Cell Death Modalities in Breast Cancer Cell Lines

et al. 2021

View full text Add to dashboard Cite

α-Mangostin (AMG) is a potent anticancer xanthone that was discovered in mangosteen (Garcinia mangostana Linn.). AMG possesses the highest opportunity for chemopreventive and chemotherapeutic therapy. AMG inhibits every step in the process of carcinogenesis. AMG suppressed multiple breast cancer (BC) cell proliferation and apoptosis by decreasing the creation of cancerous compounds. Accumulating BC abnormalities and their associated molecular signaling pathways promotes novel treatment strategies. Chemotherapy is a commonly used treatment; due to the possibility of unpleasant side effects and multidrug resistance, there has been substantial progress in searching for alternative solutions, including the use of plant-derived natural chemicals. Due to the limitations of conventional cancer therapy, nanotechnology provides hope for effective and efficient cancer diagnosis and treatment. Nanotechnology enables the delivery of nanoparticles and increased solubility of drugs and drug targeting, resulting in increased cytotoxicity and cell death during BC treatment. This review summarizes the progress and development of AMG’s cytotoxicity and the mechanism of death BC cells. The combination of natural medicine and nanotechnology into a synergistic capital will provide various benefits. This information will aid in the development of AMG nanoparticle preparations and may open up new avenues for discovering an effective BC treatment.

show abstract

“…Triple-negative breast cancers (TNBC) constitute around 15% of all cases of breast cancer; globally, it is the most complex and aggressive type of breast cancer encountered in women. The TNBC is defined by its lack of expression of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) [12,13]. Tumors with a triple negative phenotype are characterized by poor clinical prognostic features; they are usually larger in size, higher in grade, with earlier lymph node involvement, and exhibit aggressive tumor behavior, with very poor outcomes for patients [14].…”

Section: Introductionmentioning

confidence: 99%

Comparison of PLA-Based Micelles and Microspheres as Carriers of Epothilone B and Rapamycin. The Effect of Delivery System and Polymer Composition on Drug Release and Cytotoxicity against MDA-MB-231 Breast Cancer Cells

et al. 2021

View full text Add to dashboard Cite

Co-delivery of epothilone B (EpoB) and rapamycin (Rap) increases cytotoxicity against various kinds of cancers. However, the current challenge is to develop a drug delivery system (DDS) for the simultaneous delivery and release of these two drugs. Additionally, it is important to understand the release mechanism, as well as the factors that affect drug release, in order to tailor this process. The aim of this study was to analyze PLA–PEG micelles along with several types of microspheres obtained from PLA or a mixture of PLA and PLA–PEG as carriers of EpoB and Rap for their drug release properties and cytotoxicity against breast cancer cells. The study showed that the release process of EpoB and Rap from a PLA-based injectable delivery systems depends on the type of DDS, morphology, and polymeric composition (PLA to PLA–PEG ratio). These factors also affect the biological activity of the DDS, because the cytotoxic effect of the drugs against MDA-MB-231 cells depends on the release rate. The release process from all kinds of DDS was well-characterized by the Peppas–Sahlin model and was mainly controlled by Fickian diffusion. The conducted analysis allowed also for the selection of PLA 50/PLA–PEG 50 microspheres and PLA–PEG micelles as a promising co-delivery system of EpoB and Rap.

show abstract

Triple-Negative Breast Cancer Comparison With Canine Mammary Tumors From Light Microscopy to Molecular Pathology

Cited by 15 publications

References 171 publications

Liquid Biopsy as a Diagnostic and Prognostic Tool for Women and Female Dogs with Breast Cancer

Liquid Biopsy as a Diagnostic and Prognostic Tool for Women and Female Dogs with Breast Cancer

α-Mangostin Nanoparticles Cytotoxicity and Cell Death Modalities in Breast Cancer Cell Lines

Comparison of PLA-Based Micelles and Microspheres as Carriers of Epothilone B and Rapamycin. The Effect of Delivery System and Polymer Composition on Drug Release and Cytotoxicity against MDA-MB-231 Breast Cancer Cells

Contact Info

Product

Resources

About