Trimethylselenonium ion determination in human urine by high-performance liquid chromatography–hydride generation–atomic fluorescence spectrometry optimization of the hydride generation step

Atomic spectrometry update: review of advances in the analysis of clinical and biological materials, foods and beverages

“…The identification and quantification of Se metabolites in human urine is still an open field of research, considering their analytical difficulties. Slejkovec et al 151 evaluated how to optimise the HG step as part of the determination of trimethylselenonium (TMSO) in human urine by HPLC-HG-AFS. They reported an evaluation of the analytical conditions and techniques applied in recent literature, then explored the mechanism for Se IV , SeMet and TMSO volatilisation, using various mobile phases (20 mmol L −1 pyridine, 15 mmol L −1 KH 2 PO 4 or 50 mmol L −1 CH 3 COONH 4 ) and reaction coils in terms of types of Teflon (fluorinated ethylene propylene (FEP), perfluoroalkoxy (PFA), or PTFE), id (0.5–0.8 mm) and lengths (1 or 2 m).…”

Atomic spectrometry update: review of advances in elemental speciation

“…However, HPLC-HG-AFS has been also used as it also provides an adequate LOD to determine TMSe in biological fluids. Based on that, Slejkovec et al 112 report a detailed study on the conditions affecting on-line conversion of TMSe by varying the mobile phase composition (pyridine, phosphate and acetate) testing different reaction coils and several concentrations of reagents for hydride generation. The optimised HPLC-HG-AFS system consisted of a Dionex Ion Pac AS7anion exchange column with 20 mmol L −1 KH 2 PO 4 at pH 4.65 as the mobile phase at a flow-rate of 1 mL min −1 .…”