Triggering of apoptosis and cell cycle arrest by fennel and clove oils in Caco-2 cells: the role of combination

El-Garawani, Islam; El-Nabi, Sobhy Hassab; Dawoud, Gouda T. M.; Esmail, Shaimaa M; Moneim, Ahmed E. Abdel

doi:10.1080/15376516.2019.1650149

Cited by 16 publications

(10 citation statements)

References 39 publications

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“…We analysed the cell cycle to investigate the growth inhibitory effects of SLNs against the MCF‐7 cancer cell line by flow cytometry, showing that the synthesised SLNs promoted a dose‐dependent inhibitory effect on the S phase of the cell cycle. The apoptotic and cell‐cycle inhibitory effects of the SLNs could be key mechanisms through which these NPs affected the cellular growth of cancer cells [ 64 ].…”

Section: Resultsmentioning

confidence: 99%

Biosynthesis and characterisation of solid lipid nanoparticles and investigation of toxicity against breast cancer cell line

Sharifalhoseini

Es‐haghi

Vaezi

et al. 2021

IET Nanobiotechnology

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Solid lipid nanoparticles (SLNs) comprise non-toxic surface-active lipidic agents combined with appropriate ratios of drugs or essential oils. The goal of this research was to investigate the effects of the SLN synthesised using essential oils of Foeniculum vulgare on the MCF-7 breast cancer cell line. SLNs were prepared by homogenisation and ultrasound techniques and characterised by dynamic light scattering (DLS), zeta potential assessment, and transmission electron microscopy (TEM). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MTT assay), flow-cytometry, and Acridine-Orange assay were employed for assessing the biological activities of the SLNs. The average particle size was 55.43 nm and the net surface charge was −29.54 ± 11.67 mV. TEM showed that the mean particle size was 33.55 nm and the synthesised SLNs had a uniform round morphology. The MTT assay showed that the prepared SLNs had high toxicity against MCF-7 cells and low toxicity against normal HUVECs cells. Flowcytometry revealed a noteworthy rise in the subG1 peak of the cell cycle in the cancer cells treated with SLNs compared to the controls, indicating apoptosis in cancer cells. The results also showed discolouration in SLNs-treated cells, which further confirmed the induction of apoptosis and the toxicity of the SLNs against MCF-7 cells.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Section: Resultsmentioning

confidence: 99%

Biosynthesis and characterisation of solid lipid nanoparticles and investigation of toxicity against breast cancer cell line

Sharifalhoseini

Es‐haghi

Vaezi

et al. 2021

IET Nanobiotechnology

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“…Clove’s crude ethyl alcohol extract has suppressed the proliferation of human gastric cancer (AGS) cells, possibly due to the induction of apoptosis [ 51 ]. The anticancer effects of fennel and clove oil have been investigated on Caco-2 cells and normal human lymphocytes, and the oil mixture has applied selective cytotoxicity to human epithelial colorectal adenocarcinoma Caco-2 cells through cell cycle arrest and apoptosis [ 26 ].…”

Section: Main Textmentioning

confidence: 99%

The nanocomposites designs of phytomolecules from medicinal and aromatic plants: promising anticancer-antiviral applications

Kaplan

2022

Beni-Suef Univ J Basic Appl Sci

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Background Nowadays, researchers are moving toward a herbal approach to cancer treatment because of the harmful effects of synthetic anti-tumor drugs. The evaluation of active compounds with plant origin may help in the remedy of human illnesses in the future. These active compounds have direct or indirect curative efficacies on difficult to cure diseases such as cancer. Investigation of nanoforms of these active compounds is one of the curious topics of the scientific community. Main body Saffron and its components obtained from Crocus sativa, essential oils obtained from lavender, Syzygium aromaticum called cloves and Beta vulgaris are known for their anticancer effects. Nano-drugs are designed to increase the anticancer activity of plant-derived drugs. Herbal extracts operate very great in the production of nanoparticles. The aim is to ensure that only the nano-drug is delivered to the tumor site. Furthermore, nanoparticles have hazardous effects when analyzed at elevated doses, but this issue can be doped together with plant extracts. Short conclusions The nanocomposites (graphene oxide, solid lipid nano and nanoemulsion) of phytomolecules obtained from saffron, clove, lavender and red beet may be effective in minimizing these toxic effects. In the near future, detecting the anticancer molecular mechanisms of these naturally derived compounds and nanocomposites could contribute to further cancer research. Apart from these, these compounds and its nanocomposites could have antiviral effects against today's threat covid-19 virus. Consequently, more promising anticancer and antiviral agents would be discovered. Graphical abstract

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“…The biological applications of various natural products were the interest of researchers decades ago. Natural product extracts can exert chemoprotective [1,2], antigenotoxic [3], antihelminthic [4] and anticancer potency [5][6][7][8][9]. Similarly, several bioactive compounds were identified from insects and have elicited significant bioactivities including anticancer [10][11][12], antihyperlipidemic [13], antiulcer [14], cardioprotective [15], antidiabetic [16], antimicrobial [17,18] and anti-inflammatory [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Antioxidant and Cytotoxic Potentials of Lipopolysaccharides-Injected Musca domestica Larvae

et al. 2020

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The usage of insects as a sustainable and functional natural products resource is a new promise in complementary and alternative medicine. The present study aimed to investigate the ability of Musca domestica (housefly) larval hemolymph (insect blood) to display the enhanced in vitro antioxidant and cytotoxic effects. The oxidative stress (OS) was elicited by inducing lipopolysaccharides (LPS) treatment as an exogenous stressor. Determination of superoxide dismutase 1 (SOD1), glutathione (GSH), malondialdehyde (MDA) and total antioxidant capacity (TAC), and mRNA and protein expressions of SOD1, was investigated as confirmatory markers of oxidative stress induction. Cytotoxicity on cancerous MCF-7 and normal Vero cells were also evaluated using an MTT assay at 24 h post-injection. The injection of LPS induced a significant (p < 0.05) increase in SOD, GSH and TAC, whereas, the MDA was diminished. Hemolymph was collected from normal and treated larvae after 6, 12 and 24 h. The M. domestica superoxide dismutase (MdSOD1) transcripts were significantly (p < 0.05) upregulated 6 and 12 h post-treatment, while a significant downregulation was observed after 24 h. Western blot analysis showed that MdSOD1 was expressed in the hemolymph of the treated larvae with an increase of 1.2 folds at 6 and 12 h and 1.6 folds at 24 h relative to the control group. LPS-treated larval hemolymphs exhibited significant cytotoxicity with respect to the untreated ones against MCF-7 while Vero cells showed no cytotoxicity for both hemolymphs. The DPPH free radical scavenging activity was examined and a significant antioxidant potential potency was observed at 6 h (50% maximal inhibitory concentration (IC50): 63.3 ± 3.51 µg/mL) when compared to the control M. domestica larval hemolymph (IC50: 611.7 ± 10.41 µg/mL). Taken together, M. domestica larval hemolymph exhibited enhanced antioxidant and consequently increased cytotoxic capacities under stressed conditions.

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Triggering of apoptosis and cell cycle arrest by fennel and clove oils in Caco-2 cells: the role of combination

Cited by 16 publications

References 39 publications

Biosynthesis and characterisation of solid lipid nanoparticles and investigation of toxicity against breast cancer cell line

Biosynthesis and characterisation of solid lipid nanoparticles and investigation of toxicity against breast cancer cell line

The nanocomposites designs of phytomolecules from medicinal and aromatic plants: promising anticancer-antiviral applications

Enhanced Antioxidant and Cytotoxic Potentials of Lipopolysaccharides-Injected Musca domestica Larvae

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