Triggering Cell Adhesion, Migration or Shape Change with a Dynamic Surface Coating

Dongen, Stijn F. M. van; Maiuri, Paolo; Marie, Emmanuelle; Tribet, Christophe; Piel, Matthieu

doi:10.1002/adma.201204474

Cited by 68 publications

(87 citation statements)

References 43 publications

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“…Moreover, specific cellular processes such as division or migration could be controlled by defining the cell adhesion geometry, mimicking the spatial constraints that a cell is exposed to inside a tissue. 53,54,[56][57][58] Indeed, the combination of patterning techniques with mechanical mapping has provided new insights in the identification of local mechanical heterogeneities of cells. Park et al investigated 5 local cell stiffness in relation to the remodeling rate of the CSK actin density and prestress, and provided evidence for a linear correlation between cell stiffness and prestress.…”

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confidence: 99%

Atomic Force Microscopy Mechanical Mapping of Micropatterned Cells Shows Adhesion Geometry-Dependent Mechanical Response on Local and Global Scales

et al. 2015

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In multicellular organisms, cell shape and organization are dictated by cell-cell or cell-extracellular matrix adhesion interactions. Adhesion complexes crosstalk with the cytoskeleton enabling cells to sense their mechanical environment. Unfortunately, most of cell biology studies, and cell mechanics studies in particular, are conducted on cultured cells adhering to a hard, homogeneous, and unconstrained substrate with nonspecific adhesion sites, thus far from physiological and reproducible conditions. Here, we grew cells on three different fibronectin patterns with identical overall dimensions but different geometries (▽, T, and Y), and investigated their topography and mechanics by atomic force microscopy (AFM). The obtained mechanical maps were reproducible for cells grown on patterns of the same geometry, revealing pattern-specific subcellular differences. We found that local Young's moduli variations are related to the cell adhesion geometry. Additionally, we detected local changes of cell mechanical properties induced by cytoskeletal drugs. We thus provide a method to quantitatively and systematically investigate cell mechanics and their variations, and present further evidence for a tight relation between cell adhesion and mechanics.

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confidence: 99%

Atomic Force Microscopy Mechanical Mapping of Micropatterned Cells Shows Adhesion Geometry-Dependent Mechanical Response on Local and Global Scales

et al. 2015

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“… Surface and interface analysis is instrumental to advancing biointerface development. Detachment of RGD 108,110,120,121 Attachment of RGD 81,122 Reversible binding of RGD 89 Unblocking of RGD 74 Reversible display of RGD through self assembly -Leucine zipper domains 86,127 Reversible display of RGD through self assembly -cyclodextrins 88,112 Reversible display of RGD through conformational changes 85,98,109,111,129,130 Polymer bound electrodesregulation of glutamate 131 Reversible Enzyme release through surface porosity regulated by stress 125 …”

Section: Future Perspectivesmentioning

confidence: 99%

“…Control over cell adhesion and/or survival on a surface can be used to aid biomaterial integration in a living tissue, 79 detach laboratory cell cultures for further processing 80 or control spatial cellular organisation to create more complex artificial tissue. 81 Stimulated cell migration and polarisation may also aid the design of more complex tissue constructs and control the population of porous 3D objects with cells. Materials that control stem cell fate, including proliferation and differentiation, by recreating the stem cell niche in vitro are of major interest to develop advanced stem cell therapies.…”

Section: Biological Responsementioning

confidence: 99%

“…[86][87][88]109,111,112,127 In analogy to the pNIPAM based thermo-responsive surfaces that were designed to harvest cell sheets, 136,137 many of these cell-adhesion modulating surfaces were put forward as alternative routes to harvest cells. Notable other applications focus on the ability to spatially control the adhesion of cells to enable co-cultures of different cell types in predefined patterns 81 .…”

Section: Biological Responsementioning

confidence: 99%

“…On an azide terminated surface, patterns of fibronectin were created for the adhesion of the first cell type. 81 The azide surface can undergo a catalyst free cyclo addition with a cyclo-octyne bicyclo[6.1.0]nonyne-peptide conjugate (a click reaction), rendering the remaining surface cell adhesive and allowing spatially defined culture of a second cell line on the surface.…”

Section: Attachmentmentioning

confidence: 99%

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Regulation of Stem Cell Fate by Nanomaterial Substrates

Mashinchian

Turner

Dalby

et al. 2015

Nanomedicine (Lond.)

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Major design aspects for novel biomaterials are driven by the desire to mimic more varied and complex properties of a natural cellular environment with man-made materials. The development of stimulus responsive materials makes considerable contributions to the effort to incorporate dynamic and reversible elements into a biomaterial. This is particularly challenging for cell-material interactions that occur at an interface (biointerfaces); however, the design of responsive biointerfaces also presents opportunities in a variety of applications in biomedical research and regenerative medicine. This review will identify the requirements imposed on a responsive biointerface and use recent examples to demonstrate how some of these requirements have been met. Finally, the next steps in the development of more complex biomaterial interfaces, including multiple stimuli responsive surfaces, surfaces of 3D objects and interactive biointerfaces will be discussed.

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Stimuli‐Responsive Neuronal Networking via Removable Alginate Masks

2018

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The neuron patterning method has been applied to cultured neuronal networks to achieve better reproducibility and various experimental conditions in brain research. However, as models of dynamic environments, conventional patterned neural networks having an unalterable structure formed by a preprogrammed substrate have limitations, such as replicating the changes in the physical structure of networks in vivo. This study presents a novel culture technique that can control the location of the neuronal cell bodies constituting the network and the connection of the isolated neuronal networks at a desired time point. Alginate hydrogel, which has a cell‐repellent property and is dissolvable by weak chemical treatment, is used as a background masking material for patterning. Axon growth from neural clusters to the background area formed by a microwell‐patterned alginate layer is possible due to removal of the alginate layer, triggering synchronized spontaneous firing of the neuronal clusters. This technique was combined with a microelectrode array system to observe dynamic changes in neuronal activity according to the transformed morphology of the network. Finally, the clustered network structure formed by this technique showed greatly improved spontaneous firing rates and spike amplitudes compared to the network model prepared using an existing uniform structure.

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Triggering Cell Adhesion, Migration or Shape Change with a Dynamic Surface Coating

Cited by 68 publications

References 43 publications

Atomic Force Microscopy Mechanical Mapping of Micropatterned Cells Shows Adhesion Geometry-Dependent Mechanical Response on Local and Global Scales

Atomic Force Microscopy Mechanical Mapping of Micropatterned Cells Shows Adhesion Geometry-Dependent Mechanical Response on Local and Global Scales

Regulation of Stem Cell Fate by Nanomaterial Substrates

Stimuli‐Responsive Neuronal Networking via Removable Alginate Masks

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