Triggered and catalyzed self-assembly of hyperbranched DNA structures for logic operations and homogeneous CRET biosensing of microRNA

Bi, Sai; Yue, Shuzhen; Wu, Qiang; Ye, Jiayan

doi:10.1039/c6cc01308b

Cited by 63 publications

(30 citation statements)

References 36 publications

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“…HCR is a linear growth mechanism that responds to initiators. In recent years, more complicated monomers have been designed to develop nonlinear HCR [96]. For example, Hsing et al designed a target DNA-triggered nonlinear HCR system using two double-stranded DNA (dsDNA) substrates and two single-stranded assistants [97].…”

Section: Hybridization Chain Reaction (Hcr)mentioning

confidence: 99%

DNA Nanotechnology for Multimodal Synergistic Theranostics

Qiao

Song

et al. 2021

J. Anal. Test.

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Over the past decade, DNA nanotechnology has developed rapidly due to its unique characteristics, such as excellent biocompatibility, high programmability, good predictability, automatically chemical synthesis, and so on. So far, a variety of DNA-based nanostructures, from small to large and simple to complex, have been designed and synthesized with controllable size and shape in one, two, or three dimensions. Therefore, DNA has become a kind of competitive materials for biosensing, bioimaging and biomedicine. In particular, the integration of DNA nanotechnology with multimodal synergistic theranostics can not only achieve accurate cancer diagnosis by the sensitive and accurate detection of cancer biomarkers, but also achieve enhanced anti-cancer therapeutic efficacy, which promote the development of DNA nanotechnology and nanomedicine. In this review, we first give a comprehensive introduction of DNA nanotechnology, and then summarize the DNA self-assembly and amplification strategies for the construction of functional nanoplatforms for multimodal synergistic theranostics. Finally, the challenges and opportunities faced by DNA nanotechnology in biomedicine are discussed.

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Section: Hybridization Chain Reaction (Hcr)mentioning

confidence: 99%

DNA Nanotechnology for Multimodal Synergistic Theranostics

Qiao

Song

et al. 2021

J. Anal. Test.

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“…This system generates long nicked duplexes, providing additional output pathways (Figure D) . Moreover, the system enables intracellular nucleic acid imaging …”

Section: Cha Based Biosensors For Various Targetsmentioning

confidence: 99%

Applications of Catalytic Hairpin Assembly Reaction in Biosensing

Liu

Zhang

Xie

et al. 2019

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Nucleic acids are considered as perfect programmable materials for cascade signal amplification and not merely as genetic information carriers. Among them, catalytic hairpin assembly (CHA), an enzyme‐free, high‐efficiency, and isothermal amplification method, is a typical example. A typical CHA reaction is initiated by single‐stranded analytes, and substrate hairpins are successively opened, resulting in thermodynamically stable duplexes. CHA circuits, which were first proposed in 2008, present dozens of systems today. Through in‐depth research on mechanisms, the CHA circuits have been continuously enriched with diverse reaction systems and improved analytical performance. After a short time, the CHA reaction can realize exponential amplification under isothermal conditions. Under certain conditions, the CHA reaction can even achieve 600 000‐fold signal amplification. Owing to its promising versatility, CHA is able to be applied for analysis of various markers in vitro and in living cells. Also, CHA is integrated with nanomaterials and other molecular biotechnologies to produce diverse readouts. Herein, the varied CHA mechanisms, hairpin designs, and reaction conditions are introduced in detail. Additionally, biosensors based on CHA are presented. Finally, challenges and the outlook of CHA development are considered.

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“…In particular, protein/enzymefree amplification technologies( CHA and HCR) have been widely used in DNA-based logic systems. [44] Although most logic systems based on strand-displacement reaction employ the FRET signal strategy,o ther signal producing mechanisms are still being investigated. Deitersa nd coworkers [45] reported al ogic system that contains oligonucleotides modified with 7-amino-4-methyl coumarin (AMC) and phosphine separately,t he hybridization of which triggers the Staudinger reactionp artnersi nto close proximity and yielded small molecular output signals.…”

Section: Signals Derivedf Rom Strand-displacement Reactionsmentioning

confidence: 99%

Recent Progress in Fluorescence Signal Design for DNA‐Based Logic Circuits

Yang

Song

et al. 2019

Chemistry A European J

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DNA‐based logic circuits, encoding algorithms in DNA and processing information, are pushing the frontiers of molecular computers forward, owing to DNA′s advantages of stability, accessibility, manipulability, and especially inherent biological significance and potential medical application. In recent years, numerous logic functions, from arithmetic to nonarithmetic, have been realized based on DNA. However, DNA can barely provide a detectable signal by itself, so that the DNA‐based circuits depend on extrinsic signal actuators. The signal strategy of carrying out a response is becoming one of the design focuses in DNA‐based logic circuit construction. Although work on sequence and structure design for DNA‐based circuits has been well reviewed, the strategy on signal production lacks comprehensive summary. In this review, we focused on the latest designs of fluorescent output for DNA‐based logic circuits. Several basic strategies are summarized and a few designs for developing multi‐output systems are provided. Finally, some current difficulties and possible opportunities were also discussed.

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Triggered and catalyzed self-assembly of hyperbranched DNA structures for logic operations and homogeneous CRET biosensing of microRNA

Cited by 63 publications

References 36 publications

DNA Nanotechnology for Multimodal Synergistic Theranostics

DNA Nanotechnology for Multimodal Synergistic Theranostics

Applications of Catalytic Hairpin Assembly Reaction in Biosensing

Recent Progress in Fluorescence Signal Design for DNA‐Based Logic Circuits

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