In the history of phytopathology, microbial toxins have been the objects of extensive studies as possible pathogenicity or virulence factors for the producer pathogens. The recent development of molecular genetic techniques provided an experimental basis to thoroughly test the role of these secondary metabolites in pathogenesis. Some of them did prove to be highly associated with disease initiation or enhanced virulence in certain plant-pathogen interactions. In this review, we describe recent progresses in the field of plant-pathogen interactions focusing on two toxins; i.e., tabtoxin from Pseudomonas syringae and trichothecenes from Fusarium and other fungi. These microbial toxins have convincingly been shown to play causal roles in plant disease development. Studies on the biosynthesis and resistance mechanisms of these producers are outlined, and the significance of this knowledge is discussed in relation to practical applications in agriculture.Key Words--disease-resistant plants; gene cluster evolution; Fusarium mycotoxins; horizontal gene transfer; Pseudomonas syringae; self-protection mechanism; tabtoxin; trichothecene biosynthesis J. Gen. Appl. Microbiol., 47, 149-160 (2001) Invited Review * Address reprint requests to: Dr. Isamu Yamaguchi, Environmental Plant Research Group, Plant Science Center, RIKEN, 2-1 Hirosawa, Wako, 351-0198, Japan. E-mail: isyama@postman.riken.go.jp pathogen of the ability to produce the toxic substances in question or to endow the host with the ability to inactivate them, leaving all other properties of these organisms exactly the same in either case. Molecular genetic techniques served as powerful tools to prove that some of the toxins are indeed required for pathogenicity or virulence. Perhaps the most well-known examples for this are the host-specific toxins produced by three Cochliobolus species; i.e., HCtoxin of C. carbonum, T-toxin of C. heterostrophus, and victorin of C. victoriae, all of which proved to be the causal agents of severe blight diseases of economically important crops. The remarkable progresses in understanding the biological systems of these toxin producers have been summarized by the researchers involved (Cheng and Walton, 2000;Kodama et al., 1999;Walton, 1996;Walton and Panaccione, 1993).In this review, we deal with the recent progress of research on toxin biosynthesis and self-resistance mechanisms of plant pathogenic microorganisms. The following two sections summarize studies on two toxins, tabtoxin produced by Pseudomonas syringae and trichothecenes produced by a wide variety of fungal genera comprising mainly Fusarium.
Tabtoxin
Characteristics of tabtoxinTobacco leaves diseased by infection with wildfire bacteria result in a small necrotic spot containing bacterial cells, surrounded by a chlorotic zone that is free from bacteria. The wildfire bacterium P. syringae pv. tabaci produces a pathogenic toxin called tabtoxin. When a tobacco leaf was treated with tabtoxin, this toxin caused chlorotic spots similar to the halos on leaves infected with th...