Trichophytin Extraction: Biological Comparison of Trichophytin Extracted from
            <i>Trichophyton mentagrophytes</i>
            Grown in a Complex Medium and a Defined Medium

Ottaviano, Paul J.; Jones, Henry E.; Jaeger, June; King, Robert D.; Bibel, Debra Jan

doi:10.1128/am.28.2.271-275.1974

Cited by 6 publications

(4 citation statements)

References 13 publications

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“…Induration greater than 5 mm was considered as positive and ≤5 mm was considered negative DTH response. 11,12 Estimation of serum levels of total IgE was done by ImmunoCAP kit commercially purchased from Thermo Fisher. The test was performed as per the manufacturer's protocol (Thermo Fisher Phadia…”

Section: Intradermal Skin Testmentioning

confidence: 99%

Immunological response and clinical profile in patients with recurrent dermatophytosis

Jha

Shaw

Karim

et al. 2021

Mycoses

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Background: An alarming increase in the number of patients with chronic and recurrent dermatophytosis has invoked the need to study the immunological parameters of the host. Objectives: To evaluate delayed type of hypersensitivity (DTH) response and immediate hypersensitivity (IH) response by flow cytometry evaluation of immune cells from peripheral blood and intradermal trichophyton skin test in patients with recurrent dermatophytosis. Methods: A hundred patients with recurrent dermatophytosis and 50 controls (healthy controls and acute dermatophytosis controls) were included. Relevant risk factors for recurrence were analysed, and serum IgE levels were estimated. Flow cytometry evaluation of immune cells in peripheral blood and intradermal trichophyton skin test was done. Dermatophyte pathogens were isolated, and antifungal susceptibility was performed.Results: Trichophyton mentagrophytes complex (95.84%) and T. rubrum (4.16%) were isolated in culture. Serum IgE was elevated in 83.15% cases (p = .01). IFNγ + cells (p = .0501, p = .0001, p = .0014), Th1 cells (p = .1197, p = .0024, p = .0169), IL-17 + cells (p = .0127, p = .0006, p = .0007) and Th17 cells (p = .0634, p = .0001, p = .0054) were reduced, and IL-4 + cells (p = .0108, p = .0175, p = .0018) were increased in cases.Intradermal test demonstrated negative DTH response in all cases (p < .001, p < .001, p < .001), strongly positive IH response in 6%, and borderline positive IH response in 85% cases (p = .018, p < .001, p < .001). Topical corticosteroids application, undergarment types (tight fit), poor frequency of washing clothes, family history of tinea, sharing of towels were significant risk factors for recurrent dermatophytosis. Conclusions:Reduced IFNγ + , Th1, IL-17 + and Th17 cells population along with impaired DTH response by the intradermal test was observed in patients with recurrent dermatophytosis.

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Section: Intradermal Skin Testmentioning

confidence: 99%

Immunological response and clinical profile in patients with recurrent dermatophytosis

Jha

Shaw

Karim

et al. 2021

Mycoses

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“…For larger-scale production, strains ATCC 32903 and UW-1 were separately grown in 80 1 fermentors. Flasks containing 250 ml of liquid synthetic medium (containing dextrose, amino acids, vitamins, and trace minerals), pH 6.5 (18) were inoculated with fungal strains. The flasks were incubated at 30°C on a rotary shaker at 100 RPM for 2-3 weeks.…”

Section: Fungal Organism and Antigensmentioning

confidence: 99%

“…Dermatophyte glycopeptide antigen (GP) extracts were prepared from bulk-fermented ATCC 32903 and UW-I by previously-described methods ( 18,19). Briefly, mycelium was acetone-dried and ground to a powder.…”

Section: Fungal Organism and Antigensmentioning

confidence: 99%

The Immune Response toMicrosporum canisInduced by a Fungal Cell Wall Vaccine*

DeBoer

Moriello

1994

Veterinary Dermatology

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An experimental infection model was used to assess induction of specific immunity against Microsporum canis in cats with an M . canis cell wall vaccine preparation. Kittens 8-9 weeks old (n = 12) received five doses of either vaccine or placebo at biweekly intervals. Specific immunity was monitored via plasma anti-dermatophyte antibody titers and lymphocyte blastogenesis (LB) to dermatophyte antigens. After vaccination, cats were chauenged with viable M . canis spores, and lesion development was monitored. Vaccinated cats developed higher anti-dermatophyte IgG, but not IgM, titers than controls, beginning after the second dose of vaccine (P < 0.001). During the vaccination period, specific cellular immunity as measured by LB was absent in control cats, but developed to a limited degree in vaccinated cats (P < 0.05).After challenge with lo5 fungal spores per cat, both control and vaccinated cats developed active infections.The vaccine appeared to induce an antibody titer quantitatively similar to that produced by infection, but less measured cellular immunity than was seen with infection and recovery. These results suggest that induction of high titers of serum IgG or IgM antibody against Microsporum canis is not protective against challenge exposure.

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“…Antigen l (Tr I) was extracted with ethylene glycol ac cording to Cruickshank et al 1960 (4) and O11aviano et al 1974 (13). Ethylene glycol 200 ml was added per gram dried powder.…”

Section: Preparation Of A11tige11smentioning

confidence: 99%

An evaluation of delayed hypersensitivity in guinea pigs to various trichophytin preparations

Kaaman¹,

Lv²,

Wasserman³

1976

Acta Derm Venereol

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Guinea pigs immunized with mycelium from Trichophyton mentagrophytes in Freund's complete adjuvant were tested intradermally with the following trichophytin preparations: TrI extracted by ethylene glycol, TrII extracted by phenol and TrII, a commercially obtained preparation fromS√§chsisches Serumverk KG. In our experience, TrI was found to be superior to the other preparations. It showed signifiicant delayed skin reactions in all sensitized animals and none in the controls. Lymphocyte stimulation test with this preparation (TrI) gave positive results.

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Trichophytin Extraction: Biological Comparison of Trichophytin Extracted from Trichophyton mentagrophytes Grown in a Complex Medium and a Defined Medium

Cited by 6 publications

References 13 publications

Immunological response and clinical profile in patients with recurrent dermatophytosis

Immunological response and clinical profile in patients with recurrent dermatophytosis

The Immune Response toMicrosporum canisInduced by a Fungal Cell Wall Vaccine*

An evaluation of delayed hypersensitivity in guinea pigs to various trichophytin preparations

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