An evaluation of delayed hypersensitivity in guinea pigs to various trichophytin preparations

Kaaman, T; Lv, von Stedingk; Wasserman, J

doi:10.2340/0001555556283287

Acta Derm Venereol

1976

DOI: 10.2340/0001555556283287

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An evaluation of delayed hypersensitivity in guinea pigs to various trichophytin preparations

T Kaaman¹,

von Stedingk Lv²,

J Wasserman³

Abstract: Guinea pigs immunized with mycelium from Trichophyton mentagrophytes in Freund's complete adjuvant were tested intradermally with the following trichophytin preparations: TrI extracted by ethylene glycol, TrII extracted by phenol and TrII, a commercially obtained preparation fromS√§chsisches Serumverk KG. In our experience, TrI was found to be superior to the other preparations. It showed signifiicant delayed skin reactions in all sensitized animals and none in the controls. Lymphocyte stimulation test with th… Show more

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Cited by 8 publications

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Humoral and cellular immune response to a crude exo-antigen and purified keratinase of Microsporum canis in experimentally infected guinea pigs

Mignon

Leclipteux²,

Focant³

et al. 1999

Med Mycology

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In order to understand better the host-parasite relationship and to compare with previous observations in Microsporum canis naturally infected cats, the humoral and cellular immune responses to both a crude exo-antigen and a 31.5 kDa purified keratinase were evaluated in 12 M. canis experimentally infected guinea pigs. Humoral and cellular responses were assessed by ELISA from days 0 to 56 postinfection (PI) and by measurement of delayed-type hypersensitivity (DTH) responses on days 14 and 57 PI, respectively. Additionally, immunohistochemical staining was performed and demonstrated that the keratinase was produced in infected guinea pig skin, as previously reported in cats. Despite a marked interindividual variation, all the guinea pigs produced specific IgG to the crude exo-antigen from day 21 PI onwards, but no anti-keratinase IgG was detected. Strongly positive DTH responses to the exo-antigen were observed on both dates, whereas the keratinase elicited no and weak DTH on days 14 and 57 PI, respectively. These results are in agreement with those previously described for naturally infected cats, and indicate that the 31.5 kDa keratinase is not a major antigen in M. canis infection.

show abstract

Humoral and cellular immune response to a crude exo-antigen and purified keratinase of Microsporum canis in experimentally infected guinea pigs

Mignon

Leclipteux²,

Focant³

et al. 1999

Med Mycology

View full text Add to dashboard Cite

show abstract

Humoral and cellular immune response to a crude exo-antigen and purified keratinase ofMicrosporum canisin experimentally infected guinea pigs

Mignon¹,

Leclipteux²,

Focant³

et al. 1999

Med Mycol

View full text Add to dashboard Cite

In order to understand better the host-parasite relationship and to compare with previous observations in Microsporum canis naturally infected cats, the humoral and cellular immune responses to both a crude exo-antigen and a 31·5 kDa purified keratinase were evaluated in 12 M. canis experimentally infected guinea pigs. Humoral and cellular responses were assessed by ELISA from days 0 to 56 postinfection (PI) and by measurement of delayed-type hypersensitivity (DTH) responses on days 14 and 57 PI, respectively. Additionally, immunohistochemical staining was performed and demonstrated that the keratinase was produced in infected guinea pig skin, as previously reported in cats. Despite a marked interindividual variation, all the guinea pigs produced specific IgG to the crude exo-antigen from day 21 PI onwards, but no anti-keratinase IgG was detected. Strongly positive DTH responses to the exo-antigen were observed on both dates, whereas the keratinase elicited no and weak DTH on days 14 and 57 PI, respectively. These results are in agreement with those previously described for naturally infected cats, and indicate that the 31·5 kDa keratinase is not a major antigen in M. canis infection.

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Human epidermal Langerhans cells induce cellular immune response to trichophytin in dermatophytosis

Braathen

Kaaman²

1983

Br J Dermatol

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Human epidermal cell suspensions were prepared from patients sensitized to trichophytin. The cells were 75% to 95% viable, and contained 2% to 6% Langerhans cells as judged by immunofluorescence staining with an anti-DR antiserum. T lymphocytes from the same patients were co-cultured with trichophytin, with or without epidermal cells or macrophages. Proliferative T-cell response to trichophytin was obtained in cultures containing epidermal cells or macrophages. Pretreatment of the epidermal cells with anti-DR antiserum abolished the response, but normal rabbit serum did not, indicating that HLA-DR positive Langerhans cells are able to induce the cellular immune response to trichophytin. These data indicate that Langerhans cells play an important role in dermatophytosis.

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An evaluation of delayed hypersensitivity in guinea pigs to various trichophytin preparations

Cited by 8 publications

References 5 publications

Humoral and cellular immune response to a crude exo-antigen and purified keratinase of Microsporum canis in experimentally infected guinea pigs

Humoral and cellular immune response to a crude exo-antigen and purified keratinase of Microsporum canis in experimentally infected guinea pigs

Humoral and cellular immune response to a crude exo-antigen and purified keratinase ofMicrosporum canisin experimentally infected guinea pigs

Human epidermal Langerhans cells induce cellular immune response to trichophytin in dermatophytosis

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