Trichomes, essential oils and in vitro propagation of Lavandula pedunculata (Lamiaceae)

Zuzarte, Mónica; Dinis, Alexandra; Cavaleiro, Carlos; Salgueiro, Lı́gia; Canhoto, Jorge

doi:10.1016/j.indcrop.2010.07.010

Cited by 106 publications

(84 citation statements)

References 43 publications

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“…R. Zuzarte et al (2010) during morphological analysis demonstrated that trichomes on Lavandula pedunculata leaves in vitro are similar to the ones in intact plants. Histological investigation by Avato P. (2005) and Kamatou G.P.P.…”

Section: Resultsmentioning

confidence: 89%

Anatomy Features of Lavandula Angustifolia Mill. and Lavandula Hybrida Rev. Plants in Vitro

Brailko¹,

Митрофанова²,

Лесникова-Седошенко³

et al. 2017

AgricultForest

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SUMMARYLavender and lavandin are widely used in food, pharmaceutical, cosmetic industries and ornamental gardening. Gene pool collection of Lavandula angustifolia and lavandin is presented in Nikita Botanical Gardens plots and the same collection is being created in vitro.Initial explants of L. angustifolia, its cultivars 'Belyanka', 'Record' and L. hybrida -'Rabat' and 'Snezhnyi Bars' were introduced in vitro. Plant regeneration occurred on MS medium supplemented with 0.3-0.5 mg l -1 kinetin, 0.025 mg l -1 NAA, 0.25 mg l -1 GA 3 . Samples were analyzed after 2 and 8 months of culture.Structural analysis of regenerated plants vegetative organs enabled assessment of their morphogenetic capacity. Under in vitro culture 2-5 microshoots/explants were produced with the height of 2.3 -8.2 cm. Leaves were opposite, sessile linear or linear-lanceolate, with entire edges curved outward, densely pubescent, and 10-30 leaves/microshoot. Leaf lamina length was 0.9-1.5 cm. Leaves were bifacial, amphystomatic, lamina thickness -132-215 µm, palisade rate was 0.38-0.41 after 2 months culture and 0.56-0.58 -after 8 months. Epidermal thickness was 8-19 µm, cells were relatively small with thin, cellulose walls covered with cuticle. Stomata are of anomocyte type, small, raised above the epiderm cells, 48-150 pcs/mm 2 on adaxial surface, 115-432 pcs/mm 2 -on abaxial one. Essential oils are produced in simple and glandular, unicellular and multicellular hairs of epidermal origin, located above the leaf chlorophyll tissue. Features of xeromorphic structure appeared at a long-term in vitro cultivation.

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Section: Resultsmentioning

confidence: 89%

Anatomy Features of Lavandula Angustifolia Mill. and Lavandula Hybrida Rev. Plants in Vitro

Brailko¹,

Митрофанова²,

Лесникова-Седошенко³

et al. 2017

AgricultForest

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“…Taha and Haron (2008) suggest that morphological and anatomical differences can be seen in the structure of leaves of plants cultivated under field and in vitro conditions, related to the conditions of their growth and development. Zuzarte et al (2008Zuzarte et al ( , 2010 evaluated the structure and appearance of trichomes in L. pedunculata and observed that the trichomes, as well as the essential oils accumulated therein, from in vitro plants are identical to those from fieldgrown plants. In the present study, the diameter, as well as the number of trichomes per 1 mm 2 , were compared.…”

Section: Micromorphological Analysismentioning

confidence: 99%

“…Thus far, several attempts have been made to propagate Lavandula plants in vitro, e.g. such species as: L. dentata (Sudriá et al 1999, Sudriá et al 2001, L. pedunculata (Zuzarte et al 2010), L. viridis (Nogueira and Romano 2002), and L. angustifolia (Falk et al 2009). However, the antimicrobial and antioxidant properties of the essential oils isolated from them have not been determined.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and antimicrobial activities of Lavandula angustifolia Mill. field-grown and propagated in vitro

Andrys

Kulpa

Grzeszczuk

et al. 2017

Folia Horticulturae

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In the study, micropropagation of three varieties of Lavandula angustifolia was developed, and the appearance of trichomes, antioxidant activity of extracts and antimicrobial activity of essential oils isolated from plants growing in field conditions and in vitro cultures were compared. The study evaluated the number of shoots, and the height and weight of the plants grown on media with additions of BAP, KIN and 2iP. The greatest height was attained by the lavenders growing on MS medium with the addition of 1 mg dm -3 2iP -'Ellagance Purple'. The greatest number of shoots was developed by the 'Ellagance Purple' and 'Munstead' plants growing on the medium with 2 mg dm -3 BAP. The highest weight was attained by the plants growing on the medium with the highest concentration of BAP -3 and 5 mg dm . Moreover, the present study determined the influence of media with the addition of different concentrations of IBA and media with a variable mineral composition (½, ¼, and complete composition of MS medium) and with the addition of IBA or NAA for rooting. The majority of the media used had a positive influence on the development of the root system. The longest root system was observed in 'Ellagance Purple' growing on the medium composed of ¼ MS with 0.2 mg dm -3 NAA. All the examined oils exhibited activity towards S. aureus, S. epidermidis, P. aeruginosa, E. coli and C. albicans. The majority of the essential oils isolated from the plants propagated in vitro exhibited stronger antimicrobial activity than the field-grown plants. The plants propagated under in vitro conditions demonstrated considerably higher antioxidant activity as compared with the field-grown plants, which was determined using the DPPH, FRAP and ABTS assay.

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“…Therefore, vegetative propagation is the most effective to produce a large number of uniform individuals displaying a genotype of interest. Tissue culture techniques have been applied to Lavandula plants (Quazi 1980;Calvo and Segura 1989;Jordan et al 1990;Gras and Calvo 1996;Andrade et al 1999;Echeverrigaray et al 2005;Zuzarte et al 2010;Machado et al 2011;). However, the performance and survival of in vitro cultures of many plant species are often hampered by the phenomenon of hyperhydricity (Debergh et al 1992), including lavender species.…”

Section: Lavandula Angustifolia Mill (Syn L Officinalismentioning

confidence: 99%

Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill.

Machado

Silva

Biasi

et al. 2014

Braz. arch. biol. technol.

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Trichomes, essential oils and in vitro propagation of Lavandula pedunculata (Lamiaceae)

Cited by 106 publications

References 43 publications

Anatomy Features of Lavandula Angustifolia Mill. and Lavandula Hybrida Rev. Plants in Vitro

Anatomy Features of Lavandula Angustifolia Mill. and Lavandula Hybrida Rev. Plants in Vitro

Antioxidant and antimicrobial activities of Lavandula angustifolia Mill. field-grown and propagated in vitro

Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill.

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