Trichoderma-Based Biopreparation with Prebiotics Supplementation for the Naturalization of Raspberry Plant Rhizosphere

Abstract

The number of raspberry plants dying from a sudden outbreak of gray mold, verticillium wilt, anthracnosis, and phytophthora infection has increased in recent times, leading to crop failure. The plants suffer tissue collapse and black roots, symptoms similar to a Botrytis–Verticillium–Colletotrichum–Phytophthora disease complex. A sizeable number of fungal isolates were acquired from the root and rhizosphere samples of wild raspberries from different locations. Subsequent in vitro tests revealed that a core consortium of 11 isolates of selected Trichoderma spp. was the most essential element for reducing in phytopathogen expansion. For this purpose, isolates were characterized by the efficiency of their antagonistic properties against Botrytis, Verticillium, Colletotrichum and Phytophthora isolates and with hydrolytic properties accelerating the decomposition of organic matter in the soil and thus making nutrients available to plants. Prebiotic additive supplementation with a mixture of adonitol, arabitol, erythritol, mannitol, sorbitol, and adenosine was proven in a laboratory experiment to be efficient in stimulating the growth of Trichoderma isolates. Through an in vivo pathosystem experiment, different raspberry naturalization-protection strategies (root inoculations and watering with native Trichoderma isolates, applied separately or simultaneously) were tested under controlled phytotron conditions. The experimental application of phytopathogens attenuated raspberry plant and soil properties, while Trichoderma consortium incorporation exhibited a certain trend of improving these features in terms of a short-term response, depending on the pathosystem and naturalization strategy. What is more, a laboratory-scale development of a biopreparation for the naturalization of the raspberry rhizosphere based on the Trichoderma consortium was proposed in the context of two application scenarios. The first was a ready-to-use formulation to be introduced while planting (pellets, gel). The second was a variant to be applied with naturalizing watering (soluble powder).

Potential biocontrol efficiency of Trichoderma species against oomycete pathogens

“…Trichoderma is considered the representative of beneficial fungi. The members of this genus are a great source of enzymes, antibiotics, plant growth promoters, soil remediation agents, and commercial biological fungicides, while some of them have important scientific research value and economic significance in the fields of agriculture, medicine, and biotechnology (Daniel and Filho, 2007 ; Brotman et al, 2010 ; Oszust et al, 2021 ). For agricultural production, Trichoderma is often used as bio-organic fertilizer and fungicide, which has a positive impact on host plants.…”

Performance appraisal of Trichoderma viride based novel tablet and powder formulations for management of Fusarium wilt disease in chickpea

“…Clay is generally used in formulations as a filler material (Le Bras and Bourbigot, 1996). Biopolymer has been extensively used in the development of biocontrol formulations due to their nontoxicity, wider availability, and cost-effectiveness (Mukhopadhyay et al, 2019;Meena et al, 2020;Oszust et al, 2021). The presence of xerogel in the formulation matrix may provide a sustained moisture-enriched environment during storage (Mukhopadhyay et al, 2020) and supply moisture to the crop after getting released to the field through formulation application (Akhter et al, 2004;Prakash et al, 2021).…”

Metalaxyl Resistance of Phytophthora palmivora Causing Durian Diseases in Thailand

“…For example, Ramallo et al [43] reported that potassium phosphite presents a complex mode of action against the Phytophthora brown rot of lemons in pre-and postharvest applications. Moreover, durian growers should introduce integrated approaches with resistant cultivars, cultural practices, or biological control [5,44,45]. We conclude that metalaxyl use should be considered carefully, as it could increase the management costs for durian production in southern Thailand.…”