Performance appraisal of Trichoderma viride based novel tablet and powder formulations for management of Fusarium wilt disease in chickpea

Pradhan, Prakash Chandra; Mukhopadhyay, Arkadeb; Kumar, Raman; Kundu, Aditi; Patanjali, Neeraj; Dutta, Anirban; Kamil, Deeba; Bag, T. K.; Aggarwal, Rashmi; Bharadwaj, C.; Singh, Pradeep K.; Singh, Anupama

doi:10.3389/fpls.2022.990392

Cited by 10 publications

(7 citation statements)

References 46 publications

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“…There are no prior studies on the production of heptadecanoic acid, methyl ester by Bacillus strains. However, it was detected by GC-MS emitted from Trichoderma viride , which is used as a chickpea seed protectant against Fusarium wilt disease ( 84 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of bioactive compounds of Bacillus velezensis HNA3 that contribute to its dual effects as plant growth promoter and biocontrol against post-harvested fungi

Zaid,

Li,

Yang

et al. 2023

Microbiol Spectr

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Bacillus velezensis HNA3 displayed dual bioactivities as a plant growth promoter and a biocontrol agent against five isolated post-harvest phytopathogens: Alternaria alternata , Cladosporium cladosporioides , Penicillium expansum , Monilinia fructicola , and Fusarium oxysporum . Moreover, the volatile organic compounds (VOCs) emitted by HNA3 exhibited remarkable efficacy, achieving 100% fungal inhibition against Alternaria alternata and Cladosporium cladosporioides . This is the initial study to demonstrate the HNA3-emitted VOCs effect on enhancing soybean seedling growth and seed germination by breaking seed dormancy and improving root construction. Semi-VOCs and emitted VOCs were identified by two methods of extraction coupled with gas chromatography-mass spectrometry. A total of 14 main VOCs were detected; phenol,2,4-bis(1,1-dimethylethyl) and 1,2-benzenedicarboxylic acid are the most abundant semi-VOCs, both are known to have anti-fungal and plant growth-promoting properties. 9-Octadecenoic acid (z)-, methyl ester is the most common volatile compound emitted by HNA3 followed by hexadecanoic acid, methyl ester and heptadecanoic acid, methyl ester. It is noteworthy that heptadecanoic acid, methyl ester has not been previously detected in Bacillus spp. Pure 9-octadecenoic acid (z)-, methyl ester, as well as heptadecanoic acid, methyl ester, exhibited inhibitory effects on the fungal growth of all isolated phytopathogens. Interestingly, when combined, these compounds synergistically eradicated fungal growth. Indole acetic acid and serotonin, both classified as plant growth-promoting indoles, have been identified within HNA3. Moreover, during the phytopathogen inhibition stage, HNA3 demonstrated upregulation in gene expression associated with non-VOCs, including bacillibactin, bacillomycin, bacilysin, and surfactin. This study publicized an extensive variety of bioactive compounds produced by HNA3 liable for its bioactivities. IMPORTANCE The current study is an extension to our previous work on the plant growth-promoting rhizobacteria (PGPR) Bacillus velezensis HNA3 strain, which comes to confirm and reveals the huge stock of active secondary metabolites produced by HNA3. HNA3-emitted volatile organic compounds (VOCs) have demonstrated the capacity to impede the growth of phytopathogens affecting some fruits and vegetables, even in the absence of direct contact. Additionally, these volatiles enhanced soybean seed germination by breaking seed dormancy and inducing root system development. Furthermore, they promoted seedling growth, giving it prominence in soybean cultivation. The relevance of active volatiles derives from the fact that they can be developed as natural-safe biocontrol agents and plant promoters. This research validates the remarkable bioactivities exhibited by the Bacillus velezensis HNA3 and their potential applications in agriculture as an inoculant, encompassing biocontrol, plant growth promotion, and seed germination activities, thereby offering a safer alternative to hazardous chemicals.

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Section: Discussionmentioning

confidence: 99%

Identification of bioactive compounds of Bacillus velezensis HNA3 that contribute to its dual effects as plant growth promoter and biocontrol against post-harvested fungi

Zaid,

Li,

Yang

et al. 2023

Microbiol Spectr

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“…Although members of Malassezia restricta found in the HL stage are not well characterized in teleosts, there are reports of this species causing infective endocarditis in humans 48 . On the other hand, Trichoderma viride (abundant in HL) is known to be a potent antifungal species that efficiently kills pathogenic fungi in plants through its extracellular secretions consisting mainly of the enzymes chitinases and β-1,3-glucanases 49 . It would be relevant to investigate whether the salmon phylotype has similar potential for pathogen control.…”

Section: Discussionmentioning

confidence: 99%

Unravelling the temporal and spatial variation of fungal phylotypes from embryo to adult stages in Atlantic salmon

Lokesh,

Siriyappagouder,

Fernandes

2024

Sci Rep

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Early microbial colonization has a profound impact on host physiology during different stages of ontogeny. Although several studies have focused on early bacterial colonization and succession, the composition and role of fungal communities are poorly known in fish. Here, we sequenced the internal transcribed spacer 2 (ITS2) region of fungi to profile the mycobiome associated with the eggs, hatchlings and intestine of Atlantic salmon at various freshwater and marine stages. In most of the stages studied, fungal diversity was lower than bacterial diversity. There were several stage-specific fungal phylotypes belonging to different stages of ontogeny but some groups, such as Candida tropicalis, Saccharomyces cerevisiae, Alternaria metachromatica, Davidiella tassiana and Humicola nigrescens, persisted during successive stages of ontogeny. We observed significant changes in the intestinal fungal communities during the first feeding. Prior to first feeding, Humicola nigrescens dominated, but Saccharomyces cerevisiae (10 weeks post hatch) and Candida tropicalis (12 weeks post hatch) became dominant subsequently. Seawater transfer resulted in a decrease in alpha diversity and an increase in Candida tropicalis abundance. We also observed notable variations in beta diversity and composition between the different farms. Overall, the present study sheds light on the fungal communities of Atlantic salmon from early ontogeny to adulthood. These novel findings will also be useful in future studies investigating host-microbiota interactions in the context of developing better nutritional and health management strategies for Atlantic salmon farming.

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“…Pradhan et al (2022) prepared a talc-based formulation of T. viride and tested it against Fusarium wilt disease in chickpeas. The biocontrol potential of talc-based formulation was found effective in terms of seed and soil application and for reducing the incidence of wilt in chickpeas [200]. Sundaramoorthy and Balabaskar (2013) also developed the talc-based formulation of T. harzinaum to evaluate their biocontrol efficacy against wilt of tomato caused by Fusarium oxysporum f. sp.…”

Section: Talc Based Formulationmentioning

confidence: 99%

Journey of Trichoderma from Pilot Scale to Mass Production: A Review

Kumar,

Koul,

Taak

et al. 2023

Agriculture

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Trichoderma spp. has the ability to inhibit fungal plant pathogens through several mechanisms like the production of hydrolytic enzymes, mycoparasitism, coiling, and antibiosis and is therefore recommended as a potential and native biocontrol agent for effective control of soil-transmitted diseases. Various species of Trichoderma, like T. virens, T. asperellum, T. harzianum, etc., have been explored for their biocontrol activity against phytopathogens. There are different Trichoderma species and strains with respect to plant pathogens. Efforts have been made to develop effective and efficient methods, such as microencapsulation use of different polymers, adjuvants, or carriers, to increase the shelf-life and efficacy of Trichoderma formulations. The crucial aspects for the success of a biocontrol agent include developing and validating formulations, improvement in shelf-life, cost-effectiveness, easy accessibility, improved delivery systems, broad spectrum in action, robust performance (biocontrol), and integrative strategies for sustainable disease management. This review focuses on recent developments in the isolation, identification, preservation, substrates, consortium, quality control, mass production, delivery methods, field performance, registration, and commercialization of Trichoderma formulations for strategic development of next-generation multifunctional biological control formulations.

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Performance appraisal of Trichoderma viride based novel tablet and powder formulations for management of Fusarium wilt disease in chickpea

Cited by 10 publications

References 46 publications

Identification of bioactive compounds of Bacillus velezensis HNA3 that contribute to its dual effects as plant growth promoter and biocontrol against post-harvested fungi

Identification of bioactive compounds of Bacillus velezensis HNA3 that contribute to its dual effects as plant growth promoter and biocontrol against post-harvested fungi

Unravelling the temporal and spatial variation of fungal phylotypes from embryo to adult stages in Atlantic salmon

Journey of Trichoderma from Pilot Scale to Mass Production: A Review

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