Trichobilharzia spp. in natural conditions in Annecy Lake, France

Jouet, Damien; Ferté, Hubert; Depaquit, Jérôme; Rudolfová, Jitka; Latour, P.; Zanella, Damien; Kaltenbach, Matthieu L.; Léger, N.

doi:10.1007/s00436-008-0926-3

Cited by 53 publications

(56 citation statements)

References 23 publications

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“…Later on, R. lagotis and R. labiata were identified as the intermediate hosts in the Czech Republic (R. lagotis served for routine laboratory maintenance of the parasite for many years, but without determination of the level (in %) of its susceptibility); the role of R. peregra in T. regenti transmission was weakened. Now it is re-confirmed that R. peregra, besides R. lagotis and R. labiata, can play a role as a vector of T. regenti; this is in agreement with the results from France (Jouet et al 2008). Interestingly, in Iceland, R. peregra represents the dominant snail species and hosts several schistosomes (Skírnisson et al 2009), showing that both the schistosomes and the snails can adapt to each other under specific local conditions.…”

Section: Discussionsupporting

confidence: 85%

Radix spp.: Identification of trematode intermediate hosts in the Czech Republic

Huňová

Kašný

Hampl

et al. 2012

Acta Parasitologica

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Lymnaeid snails of the genus Radix serve as intermediate hosts of some schistosomes and fasciolids. In Europe, delineation of species within the genus Radix is unresolved and, therefore, spectrum of snail hosts susceptible to trematode infections is under discussion. We used and compared three criteria for species delineation using snails collected at 43 localities. (a) Sequence analysis of ITS-2 rDNA disclosed that the collected snails belong to four species -R. auricularia (Linnaeus, 1758), R. peregra (Müller, 1774), R. lagotis (Schrank, 1803) and R. labiata (Rossmaessler, 1835) (criteria and names are based on the work of Bargues et al. 2001). Occurrence of R. peregra in the Czech Republic was confirmed by molecular data for the first time. (b) Characterization of reproductive system disclosed differences in location, size and shape of bursa copulatrix and its ductus. Unfortunately, some R. labiata specimens shared morphological features of reproductive organs with R. lagotis. (c) Statistical analysis of shell morphology proved that significant differences exist among particular species. One prediction model showed that correct classification of species may be achieved in 82-84% of cases. However, identification of individual snails in the field (without knowledge of respective snail population and use of statistical tools) still remains a complicated issue due to overlaps of shell characteristics. Concerning the role in trematode transmission, R. lagotis, R. labiata and R. peregra are susceptible to Trichobilharzia regenti. Also, successful experimental infections of R. lagotis and R. labiata by Fascioloides magna were accomplished.

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Section: Discussionsupporting

confidence: 85%

Radix spp.: Identification of trematode intermediate hosts in the Czech Republic

Huňová

Kašný

Hampl

et al. 2012

Acta Parasitologica

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“…Outbreaks can lead to the closing of swimming areas and consequently to major financial losses for touristic operators. Several foci of HCD are well known in Europe, for example in Germany (Selbach et al 2015), France ( Jouet et al 2008), the Netherlands (Schets et al 2008), and Poland (Zbikowska 2004). It is worth mentioning that in Belgium, to the authors' knowledge, no cases were ever recorded in scientific literature.…”

Section: Introductionmentioning

confidence: 99%

“…The rDNA of furcocercariae was amplified using the primers previously described (Mollaret et al 1997): C2¢B (forward) (5¢-GAA AAGTACTTTGRARAGAGA-3¢) and D2 (reverse) (5¢-TCC GTGTTTCAAGACGGG-3¢). For the amplification of the positive snails, a primer pair, previously described ( Jouet et al 2008), was used: DIX1(forward) (5¢-CGCGCTCTG GWCCKTCGCGGC-3¢) and DIX2 (reverse) (5¢-ATYTYG TYCGATTTGAGGTTG-3¢) to amplify the ITS-2 of Radix spp. For cercariae, initial denaturation at 94°C for 3 min was followed by 40 cycles of denaturation at 94°C for 45 s, annealing at 50°C for 45 s, and extension at 68°C for 2 min with a final elongation time of 10 min at 68°C.…”

Section: Molecular Analysis Of Cercariae and Positive Snailsmentioning

confidence: 99%

“…The sequences were amplified using a commercial kit Taq PCR Master Mix (Qiagen, Hilden, Germany) in a Peltier Thermal Cycler (MJ Research). A protocol previously described ( Jouet et al 2008( Jouet et al , 2009) was followed. The rDNA of furcocercariae was amplified using the primers previously described (Mollaret et al 1997): C2¢B (forward) (5¢-GAA AAGTACTTTGRARAGAGA-3¢) and D2 (reverse) (5¢-TCC GTGTTTCAAGACGGG-3¢).…”

Section: Molecular Analysis Of Cercariae and Positive Snailsmentioning

confidence: 99%

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Swimmer's Itch in Belgium: First Recorded Outbreaks, Molecular Identification of the Parasite Species and Intermediate Hosts

Caron

Cabaraux²,

Maréchal

et al. 2017

Vector-Borne and Zoonotic Diseases

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Background: Cercarial dermatitis or swimmer's itch is a skin condition in humans due to the larval forms of bird schistosomes of some species of the genus Trichobilharzia. The life cycle of these schistosomes requires freshwater snails (intermediate host) and waterfowl (definitive host). Repeated exposures to cercariae can lead to skin sensitization with the induction of pruritic skin lesions. Methods: We describe, in this study, two outbreaks of human cercarial dermatitis at the Eau d'Heure Lakes, Belgium. In July and August 2012, a total of, respectively, 78 and 10 people reported a sudden skin rash accompanied by pruritus following recreational activities in the Plate Taille Lake. However, no ocellate furcocercariae were detected following light exposure of the snails collected between September 2012 and September 2013 (n = 402). No outbreaks were recorded in 2013 and 2014. In August 2015, about 30 new cases were recorded. Snails were collected (n = 270) in different locations around the lake. PCR was used to identify accurately the intermediate hosts and the parasite species involved. Results: After light exposure, seven Radix spp. (2.6%) shed ocellate furcocercariae. Molecular identification based on the rDNA internal transcribed spacer 2 sequence ascribed the infected snails to R. balthica ( = R. peregra = R. ovata) (6/7) and R. auricularia (1/7). Based on the amplification of the D2 domain of the 28S rDNA, the cercariae were shown to belong to two different haplotypes of Trichobilharzia franki. Conclusions: This is the first record in Belgium of T. franki and associated skin condition.

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“…Larval stages of F. hepatica have been recorded in natural conditions in Lymnaea truncatula, Omphiscola glabra (Muller, 1774; Rondelaud et al 2001;Dreyfuss et al 2005) and even in unspecific Planorbis leucostoma Millet, 1813 (Dreyfuss et al 2002). Also conducted from a medical point of view was research on snails infected with avian schistosomes-an etiological factor of "swimmers' itch" in Lake Annecy (eastern France; Jouet et al 2008). Extremely interesting observations taking into account parasites as a biotic factor affecting snail populations living in Lake Combourg and Mont Saint-Michel Bay were made by Gerard (1997Gerard ( , 1998Gerard ( , 2001aGerard ( , b, 2003.…”

Section: Countries In Which Research Has Been Conducted On the Naturamentioning

confidence: 98%

One hundred years of research on the natural infection of freshwater snails by trematode larvae in Europe

Żbikowska

Nowak

2009

Parasitol Res

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Research on the infection of snails by trematodes has been conducted in Europe for over a hundred years. The initial poor knowledge of the intra-molluscan stages of these parasites together with the difficulty of classifying them constituted a serious obstacle to the undertaking of integrated parasitological and malacological efforts to gain a better understanding of the phenomenon. The compilation of morphological and anatomical results of research on trematode larvae resulted in the publication of keys to designate species of parasites, but was not sufficient to encourage malacologists to collaborate with parasitologists. This paper undertakes to collect data published over the last hundred years on the natural infection of European populations of freshwater snails by trematode larvae. The aim of this undertaking is to make researchers of malacofauna and, above all, experts on freshwater snails aware of the scale of the problem of molluscs being exploited as intermediate hosts of trematodes and, consequently, to encourage parasitologists and malacologists to collaborate on this phenomenon that is crucial for both parasites and hosts.

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Trichobilharzia spp. in natural conditions in Annecy Lake, France

Cited by 53 publications

References 23 publications

Radix spp.: Identification of trematode intermediate hosts in the Czech Republic

Radix spp.: Identification of trematode intermediate hosts in the Czech Republic

Swimmer's Itch in Belgium: First Recorded Outbreaks, Molecular Identification of the Parasite Species and Intermediate Hosts

One hundred years of research on the natural infection of freshwater snails by trematode larvae in Europe

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