Trichloroethylene removal and oxidation toxicity mediated by toluene dioxygenase of Pseudomonas putida

Heald, S. C.; Jenkins, Richard O.

doi:10.1128/aem.60.12.4634-4637.1994

Cited by 76 publications

(14 citation statements)

References 15 publications

(13 reference statements)

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“…An additional objective of this study was to confirm and expand upon the recent findings that Heald and Jenkins (21) obtained with P. putida and that McClay et al (35) obtained with P. mendocina KR1 and Pseudomonas sp. strain ENVPC5 which showed that TCE can act as an inducer of tolueneoxidizing activity.…”

supporting

confidence: 61%

“…Trichloroethylene (TCE), a suspected carcinogen (43) and U.S. Environmental Protection Agency priority pollutant (60), is the most commonly reported volatile organic contaminant of groundwater (51). TCE can be degraded by means of a cooxidation reaction which has been shown to be catalyzed by both toluene dioxygenase (9,21,68) and monooxygenase (29,55,66) enzyme systems found in toluene-oxidizing bacteria. Linkage of TCE degradation with the metabolism of aromatic hydrocarbons has been established for indigenous microbial populations in soil (11,13,42) and groundwater (22)(23)(24), in which TCE removal has been shown to be stimulated by the addition of toluene or phenol.…”

mentioning

confidence: 99%

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Comparison of factors influencing trichloroethylene degradation by toluene-oxidizing bacteria

Leahy

Byrne

Olsen

1996

Appl Environ Microbiol

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The degradation of trichloroethylene (TCE) by toluene-oxidizing bacteria has been extensively studied, and yet the influence of environmental conditions and physiological characteristics of individual strains has received little attention. To consider these effects, the levels of TCE degradation by strains distinguishable on the basis of toluene and nitrate metabolism were compared under aerobic or hypoxic conditions in the presence and absence of nitrate and an exogenous electron donor, lactate. Under aerobic conditions with tolueneinduced cells, strains expressing toluene dioxygenases (Pseudomonas putida F1, Pseudomonas sp. strain JS150, Pseudomonas fluorescens CFS215, and Pseudomonas sp. strain W31) degraded TCE at low rates, with less than 12% of the TCE removed in 18 h. In contrast, strains expressing toluene monooxygenases (Burkholderia cepacia G4, Burkholderia pickettii PKO1, and Pseudomonas mendocina KR1) degraded 36 to 67% of the TCE over the same period. Under hypoxic conditions (1.7 mg of dissolved oxygen per liter) or when lactate was added as an electron donor, the extent of TCE degradation by toluene-induced cells was generally lower. In the presence of lactate, degradation of TCE by denitrifying strain PKO1 was enhanced by nitrate under conditions in which dissimilatory nitrate reduction was observed. The results of experiments performed with strains F1, G4, PKO1, and KR1 suggested that TCE or an oxidation product induces toluene degradation and that TCE induces its own degradation in the monooxygenase strains. The role of TCE as an inducer of toluene oxygenase activity in PKO1 was confirmed by performing a promoter probe analysis, in which we found that TCE activates transcription from the PKO1 3-monooxygenase operon promoter.

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supporting

confidence: 61%

mentioning

confidence: 99%

Comparison of factors influencing trichloroethylene degradation by toluene-oxidizing bacteria

Leahy

Byrne

Olsen

1996

Appl Environ Microbiol

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“…The difference in growth or no growth is dependent solely on the genetic regulation of toluene 2-monooxygenase. In this context, it is of interest that TCE induces the expression of toluene dioxygenase in Pseudomonas putida UV4 (7) and of toluene 4-monooxygenase in Pseudomonas mendocina KR1 (14). These results and those of the present study highlight the importance of matching enzyme and induction specificities for optimizing the metabolic capabilities of organisms.…”

supporting

confidence: 65%

Toluene 2-Monooxygenase-Dependent Growth of Burkholderia cepacia G4/PR1 on Diethyl Ether

Hur¹,

Newman²,

Wackett³

et al. 1997

Appl Environ Microbiol

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Aerobic bacterial growth on aromatic hydrocarbons typically requires oxygenase enzymes, which are known to fortuitously oxidize nongrowth substrates. In this study, we found that oxidation of diethyl ether by toluene 2-monooxygenase supported more rapid growth of Burkholderia cepacia G4/PR1 than did the aromatic substrates n-propylbenzene and o-xylene. The wild-type Burkholderia cepacia G4 failed to grow on diethyl ether. Purified toluene 2-monooxygenase protein components oxidized diethyl ether stoichiometrically to ethanol and acetaldehyde. Butyl methyl ether, diethyl sulfide, and 2-chloroethyl ethyl ether were oxidized by B. cepacia G4/PR1.

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“…Trichloroethylene, a suspected carcinogen and U.S. Environmental Protection Agency priority pollutant, is a common groundwater and soil contaminant in the United States. Various types of aerobic bacteria, including methanotrophs [1–3], toluene oxidizers [4,5], phenol oxidizers [6], and ammonia oxidizers [1], have been shown to cometabolically degrade TCE. Growth of these bacteria on substrates other than chlorinated aliphatic compounds involve nonspecific oxygenase enzymes that are able to fortuitously degrade these compounds.…”

Section: Introductionmentioning

confidence: 99%

“…Growth of these bacteria on substrates other than chlorinated aliphatic compounds involve nonspecific oxygenase enzymes that are able to fortuitously degrade these compounds. In toluene‐degrading microorganisms, for example, TCE can be degraded by means of both dioxygenase [4,5,7,8] and mono‐oxygenase [9] enzyme systems. The relationship between cometabolic TCE transformation and toluene consumption has been described qualitatively by many researchers over the past decade [5,10–15].…”

Section: Introductionmentioning

confidence: 99%

Effect of sorption and desorption resistance on aerobic trichloroethylene biodegradation in soils

Lee

Moe

Valsaraj

et al. 2002

Enviro Toxic and Chemistry

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Biodegradation of trichloroethylene (TCE) by toluene-degrading bacteria was measured under aerobic conditions in aqueous and soil-slurry batch microcosms. For soil-phase experiments, a freshly contaminated soil and a soil containing only the desorption-resistant fraction of TCE were tested. In both cases, presence of soil resulted in biodegradation rates substantially lower than those determined in the absence of soil. In aqueous-phase experiments, an appreciable increase in the rate and extent of TCE biodegradation was observed in microcosms when toluene was added multiple times. The TCE degradation rates were clearly correlated with toluene dioxygenase (TOD) enzyme activity over time, thus providing an indication of the cometabolic pathway employed by the microbial population. In soil-slurry experiments containing freshly contaminated soil, a TCE degradation rate of approximately 150 microg TCE/kg/h was observed during the first 39-h period, and then the TCE degradation rate slowed considerably to 0.59 and 0.84 microg TCE/kg/h for microcosms receiving one and two additions of toluene, respectively. The TCE degradation rates in soil-slurry microcosms containing the desorption-resistant fraction of TCE-contaminated soil were approximately 0.27 and 0.32 microg TCE/kg/h in microcosms receiving one and two additions of toluene, respectively. It is clear from these results that mass transfer into the aqueous phase limited bioavailability of TCE in the contaminated soil.

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Trichloroethylene removal and oxidation toxicity mediated by toluene dioxygenase of Pseudomonas putida

Cited by 76 publications

References 15 publications

Comparison of factors influencing trichloroethylene degradation by toluene-oxidizing bacteria

Comparison of factors influencing trichloroethylene degradation by toluene-oxidizing bacteria

Toluene 2-Monooxygenase-Dependent Growth of Burkholderia cepacia G4/PR1 on Diethyl Ether

Effect of sorption and desorption resistance on aerobic trichloroethylene biodegradation in soils

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