Trichinocidal activity of a novel formulation of curcumin-olive oil nanocomposite in vitro

Khalifa, Marwa M.; Ramadan, Reem M.; Youssef, Fady Sayed; Abdel-Radi, Shimaa; El-Bahy, Mohamed M.; Taha, Noha

doi:10.1016/j.vprsr.2023.100880

Cited by 4 publications

(6 citation statements)

References 24 publications

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“…The absence of adult worms in intestines after 33 dpi was previously mentioned by Khalifa et al 28 , as this can be explained as a result of the effect of the drugs in addition to the normal life span of the adult worms in the intestinal tissues even in the non-treated mice.…”

Section: Discussionmentioning

confidence: 58%

“…In this study, nanomaterials’ heightened effectiveness, compared to other products supported by various researchers, is attributed to their distinctive and advanced physicochemical properties. Nanomaterials have a huge surface-to-volume ratio, high reactivity, stability, measured molecular sizes, effective bioactivity, and high bioavailability, as well as controlled delivery of loaded drugs to the target site 12 , 28 .…”

Section: Discussionmentioning

confidence: 99%

“…1 ) were extracted from naturally infected pigs' diaphragmatic muscles directly after slaughtering at a Cairo abattoir in Egypt. After inspection of suspected samples by trichinoscope, heavily infected pieces were minced, and then their larvae were extracted using the digestion technique described by Khalifa et al 28 . In brief, the macerated minced muscles were transferred to a ten-time volume of digestion solution composed of 1% pepsin HCl in 200 ml of distilled water and then incubated at 37 °C for one hour with continuous stirring.…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, pieces from the body and diaphragmatic muscles were compressed by trichinoscope plates and investigated microscopically to determine the stage of development of the cyst and the presence of early migrating larvae (Fig. 1 ) 28 . After removing the skin, internal organs, terminal parts of the feet, and the mouse heads of infected and control mice, the carcasses were weighed and then dissected into small pieces and minced before transferring the whole tissue to the digestion process.…”

Section: Methodsmentioning

confidence: 99%

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Efficacy of silver nanoparticles against Trichinella spiralis in mice and the role of multivitamin in alleviating its toxicity

Taha,

Youssef,

Auda

et al. 2024

Sci Rep

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Trichinellosis is a worldwide zoonotic disease. The majority of currently available anti-trichinellosis medications exhibit inadequate efficacy. The efficacy of a natively prepared new formulation of silver nanoparticles (Ag-NPs) was evaluated in the treatment of Trichinella spiralis (T. spiralis) infection in mice alone and combined with multivitamin-mineral (MM). After investigating the product’s biological and pharmacological characteristics, its therapeutic dose was estimated to be Ag-NPs at 21.5 mg/kg B.W. This dose was orally inoculated to experimentally infected mice at 3–5 days post-inoculation (dpi) against the mature worms, at 8–10 dpi against the newborn larvae, and at 33–35th dpi against the encapsulated larvae. Each treatment’s efficacy was assessed by scarifying control and treated mice 3 days post-treatment. The drug alone or in supplement form has a high trichinocidal effect exceeding that of the reference drug. Early treatment (3–5 dpi) by Ag-NPs or Ag-NPs + MM and albendazole revealed high efficacy against the intestinal stage, reaching 93.3%, 94.7%, and 90.6% for the three treatments, respectively. The materials causing a significant (P-value < 0.001) decrease in the mean encapsulated larvae reached 86.61%, 89.07%, and 88.84%/gm of muscles using the three treatments, respectively. Moreover, all larvae extracted from Ag-NPs-treated groups failed to induce infection post-inoculation in new mice. Additionally, combining the material with MM proved to overcome the reversible adverse effects of silver material on the estimated redox parameters and liver and kidney biomarkers, denoting its ability to alleviate Ag-NP toxicity. In conclusion, the high trichinocidal effect of Ag-NPs against the adult and encapsulated larvae during a short inoculation period introduced Ag-NPs as an alternative to other nematicidal drugs.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Efficacy of silver nanoparticles against Trichinella spiralis in mice and the role of multivitamin in alleviating its toxicity

Taha,

Youssef,

Auda

et al. 2024

Sci Rep

Self Cite

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“…Latin phrase nanus is where the word nano comes from, which is the same as dominate and denotes extremely small size (1 nm equals 10 9 m). Another emerging technology that has applications in a variety of disciplines, such as research, agriculture, and infection treatment delivery tests is nanotechnology [1]. Additionally, nanomaterials may have an impact on biomedical applications and research both in vivo and in vitro [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic and Toxicological Aspects of Some Metal Nanoparticles on The Central Nervous System: A Review

Youssef,

Fouad,

Ismail

et al. 2024

Egyptian Journal of Veterinary Sciences

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RAPIDLY developing technology, nanotechnology significantly impacts several therapeutic application domains. Nanotechnology, developed in 1974, focuses on creating materials with a size between 1 and 100 nanometres. It has applications in research, agriculture, and infection treatment delivery tests. Numerous issues pertaining to the welfare and productivity of animals can be resolved by it. Despite their distinctive benefits and uses in both the home and commercial areas, the usage of materials with diameters in nanometres have inspired concerns about worker, consumer, and environmental safety. Nanoparticles have the potential to harm humans and animals through interactions through a variety of processes due to their little dimensions and other distinctive properties. In this review article we have reported and mentioned the toxicity of different selected types of nanoparticles especially metallic nanoparticles such as gold, titanium dioxide, silver, iron oxide and manganese oxide nanoparticles and in addition, we focused on their mechanisms of action of toxicity on central nervous system especially on brain.

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Light microscopical and parasitological analyses revealed the beneficial effects of silver nanoparticles and various myrrh extracts against Trichinella spiralis infection in mice

Elossily,

Abd‐ELrahman,

Khedr

et al. 2024

Microscopy Res & Technique

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Trichinella spiralis infection is a food‐borne zoonotic disease caused by nematodes that dwell in the tissues, presenting a significant public health concern. This study aimed to evaluate the effectiveness of different treatments including silver nanoparticles (AgNPs), myrrh biosynthesized AgNPs “AgNPs synthesized using plant‐based green technologies”, myrrh extract, and myrrh essential oil, as alternative treatments against T. spiralis infection. Parasitological, histopathological, and cytotoxicity assessments were conducted to investigate the effects of various concentrations of these treatments in reducing the populations of adult worms and larvae during both the intestinal and muscular phases of T. spiralis‐infected mice. The results showed that the highest antihelminthic efficacy against the intestinal phase of T. spiralis was achieved by myrrh extract (86.66%), followed closely by AgNPs (84.96%) and myrrh AgNPs (82.51%) at higher concentrations (800 mg/kg for myrrh extract, 40 μg/mL for AgNPs, and 40 μg/mL for myrrh AgNPs). While the group treated with myrrh essential oil showed the lowest percentage of adult reduction (78.14%). However, all treatments demonstrated comparable effects in reducing the larvae population in the muscle phase. Histopathological examination of the tissues revealed compelling evidence of the effectiveness of AgNPs, particularly when prepared with myrrh. Additionally, a comprehensive assessment of the cytotoxicity of AgNPs indicated low toxicity levels. This study supports that AgNPs synthesized using plant‐based green technologies hold therapeutic potential for the treatment of T. spiralis infection. These findings present a promising avenue for the development of novel antiparasitic drugs that are both effective and safe.Research Highlights Myrrh extract has the highest antihelminthic efficacy against the intestinal phase of T. spiralis. Histopathological examination of the tissues revealed compelling evidence of the effectiveness of AgNPs, particularly when prepared with myrrh. During intestinal phase of T. spiralis, varying levels of nanoparticle precipitation were detected in the liver, brain, lung, and intestine. During the muscular phase, the highest amount of AgNPs precipitation was detected in the liver, followed by the brain, and lung.

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Trichinocidal activity of a novel formulation of curcumin-olive oil nanocomposite in vitro

Cited by 4 publications

References 24 publications

Efficacy of silver nanoparticles against Trichinella spiralis in mice and the role of multivitamin in alleviating its toxicity

Efficacy of silver nanoparticles against Trichinella spiralis in mice and the role of multivitamin in alleviating its toxicity

Therapeutic and Toxicological Aspects of Some Metal Nanoparticles on The Central Nervous System: A Review

Light microscopical and parasitological analyses revealed the beneficial effects of silver nanoparticles and various myrrh extracts against Trichinella spiralis infection in mice

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