Trichinella spiralis: Immunization of rats with an antigen fraction enriched for allergenicity

Durham, C.P.; Murrell, K. D.; Lee, C.M.

doi:10.1016/0014-4894(84)90104-8

Cited by 12 publications

(5 citation statements)

References 27 publications

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“…The changing surface of T. spiralis implices the presence of dierent antibodies in sera of infected individuals (Morakote et al 1992;Wang and Bell 1992). Additionally, after immunization with dierent fractions of T. spiralis antigen, dierent antibody levels were found (Durham et al 1984).…”

Section: Discussionmentioning

confidence: 98%

Trichinella spiralis : in vitro cytotoxicity of peritoneal cells against synchronous newborn larvae of different age

Moskwa¹

1999

Parasitology Research

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Two different immune sera obtained from mice infected with muscle larvae (ML) of Trichinella spiralis (oral infection) or injected with 20-h-old newborn larvae (NBL) via retro-orbital venous plexus (intraocular injection) were compared in antibody-dependent cellular cytotoxicity test. Four synchronous stages of NBL, 0-2 h old, 22 24 h old, 46-48 h old and 6 days old (sNBL) were used to study susceptibility of larvae to the cytotoxic reaction of peritoneal cells. Peritoneal cells adhered to and destroyed sNBL of T. spiralis only in the presence of both immune sera. Living sNBL, living sNBL covered by peritoneal cells and dead sNBL were observed in all experimental groups. The lowest percentage of dead sNBL was detected in the 22- to 24-h-old groups. A dramatic increase in mortality was observed in older groups. A greater susceptibility of sNBL to serum obtained from mice infected per os with ML was observed. Serum obtained after oral infection showed higher levels of IgG1 isotype specific to ML than serum obtained after ocular injection.

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Section: Discussionmentioning

confidence: 98%

Trichinella spiralis : in vitro cytotoxicity of peritoneal cells against synchronous newborn larvae of different age

Moskwa¹

1999

Parasitology Research

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“…Parasite-speci®c bands were observed from day 10 and the number of identi®ed antigenic bands increased during the infection. Dierent antibodies and variations in the level of antibody have been found in the sera of infected animals and also after immunisation with dierent fractions of T. spiralis antigen (Durham et al 1984). Janssen et al (1998) reported that T. spiralis infective larvae are activated very early, upon liberation of the larvae from nurse cells inside the host stomach.…”

Section: Discussionmentioning

confidence: 99%

Immune response to Trichinella spiralis larvae after treatment with the anti-allergic compound ketotifen

Doligalska¹

2000

Parasitology Research

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Ketotifen was used as an anti-allergic agent to study the relationship between eosinophil-related responses and IgG1 and IgG2a antibody responses in BALB/c mice infected with Trichinella spiralis. The results showed that leukocyte and eosinophil numbers and interleukin-5 (IL-5) concentrations in the peritoneal fluid increased after exposure to nematodes and the increases were slightly greater in animals treated with ketotifen. A decreased concentration of eosinophil peroxidase and an elevation in IgG1 accompanied the muscle phase of infection. In mice treated with ketotifen, antibody-mediated recognition of muscle larvae was delayed. The retardation of IgG1 and IgG2a responses may have been responsible for the ineffective immune response against larvae migrating into the muscle. The activation of eosinophils was accompanied by changes in IL-5 concentration, but these changes were not associated with differences in protection against infection.

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“…It will facilitate fur ther investigation of the relationship between immune status and gut sensitivity, as well as aid the purifica tion and characterisation of the allergens in tissue homogenates and excretory-secretory products. To date, the only parasite allergens described in detail are those purified from Ascaris suum [38,39] and T. spiralis [40].…”

Section: Discussionmentioning

confidence: 99%

Gut Hypersensitivity Reactions in Guinea Pigs following Vaccination or Infection with <i>Trichostrongylus colubriformis</i>

Ward

Wagland

1990

Int Arch Allergy Immunol

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A quantitative approach to measuring gut hypersensitivity responses to parasite antigens is described. Guinea pigs exposed to infection with Trichostrongylus colubriformis, were sensitised to excretory-secretory products and homogenates of third-stage larvae (L3), fourth-stage larvae (L4) and adult worms. Εhe nature of these responses and their modification by pharmacological agents were typical of immediate-type hypersensitivity reactions. Gut sensitivity was greatest in the anterior half of the small intestine, persisted for at least 12 months and was more pronounced against L3 antigens than against L4 or adult preparations. Guinea pigs injected with homogenates of L3, L4 or adult worms were sensitised to these homogenates, but not to T. colubriformis excretory-secretory products.

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Trichinella spiralis: Immunization of rats with an antigen fraction enriched for allergenicity

Cited by 12 publications

References 27 publications

Trichinella spiralis : in vitro cytotoxicity of peritoneal cells against synchronous newborn larvae of different age

Trichinella spiralis : in vitro cytotoxicity of peritoneal cells against synchronous newborn larvae of different age

Immune response to Trichinella spiralis larvae after treatment with the anti-allergic compound ketotifen

Gut Hypersensitivity Reactions in Guinea Pigs following Vaccination or Infection with <i>Trichostrongylus colubriformis</i>

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