Tricalcium phosphate solubilizing abilities ofTrichodermaspp. in relation to P uptake and growth and yield parameters of chickpea (Cicer arietinumL.)

Abstract

Nine isolates of Trichoderma spp. were investigated for their ability to solubilize insoluble phosphate in Pikovskaya's broth and were compared with an efficient phosphate-solubilizing bacterium Bacillus megaterium subsp. phospaticum PB that was used as the reference strain. All 9 Trichoderma isolates were found to solubilize insoluble tricalcium phosphate to various extents. Trichoderma viride (TV 97) (9.03 microg x mL(-1)), Trichoderma virens (PDBCTVs 12) (9.0 microg x mL(-1)), and Trichoderma virens (PDBCTVs 13) (8.83 microg x mL(-1)) solubilized 70% of that solubilized by the reference strain Bacillus megaterium (12.43 microg x mL(-1)). Pot culture and field evaluations with Trichoderma harzianum (PDBCTH 10), Trichoderma viride (TV 97), and Trichoderma virens (PDBCTVs 12) using chickpea (Cicer arietinum L.) 'Annegeri-1' as the test plant and rock phosphate as the phosphorus source showed significantly increased P uptake in plants treated with Trichoderma harzianum (PDBCTH 10) followed by Trichoderma virens (PDBCTVs 12) and Trichoderma viride (TV 97). Inoculation of Trichoderma spp. also showed increased growth and yield parameters of chickpea compared with the uninoculated controls under both glasshouse and field conditions.

Potential of three microbial bio-effectors to promote maize growth and nutrient acquisition from alternative phosphorous fertilizers in contrasting soils

“…The absence of response after BE application was observed in many other experiments [45,48,49] and in particular in soils characterized by extraordinary low levels of available P (20 mg kg −1 or lower) or that were limiting in other soil nutrients [10,50,51]. In contrast, other studies have shown that for maize and other crops, the application of BEs was more efficient in the absence of added P fertilizers [36][37][38]. However, in these cases, soil P availability was distinctly higher and P CAL ranged between 35 and 45 mg/kg.…”

“…The choice of these three BEs is based on their published ability to act as potential bio-stimulants and biofertilizers. The strain Trichoderma harzianum Rifai 1295-22 (T22) has demonstrated abilities to increase the solubilization of sparingly soluble calcium phosphates [34] and to promote plant growth in various plant species [35,36] including maize [37]. The strain Pseudomonas DSMZ 13134 is an efficient root colonizer [38] and has been shown to solubilize inorganic phosphate under in vitro conditions [39].…”

Effect of bioeffectors and recycled P-fertiliser products on the growth of spring wheat

“…The concept of BEs covers quite a diverse group of natural products [15]. In the present paper, the scope was limited to plant growth-promoting microorganisms (PGPM) focusing on plant growth-promoting rhizobacteria (PGPR) [16] and free-living fungi, such as species of the genus Trichoderma [17]. PGPM may have the potential to enhance plant uptake of P from soil [14,18,19].…”

“…PGPM may have the potential to enhance plant uptake of P from soil [14,18,19]. Improved growth under P-limiting soil conditions as a result of microbial inoculations has been observed in many different plant species, such as mung bean [17], bean [20], maize [21] and wheat [22]. In soil, a large proportion of the total P pool is not directly available for plant uptake [23], and the ability to solubilise phosphates in the rhizosphere has been viewed as an important function of PGPM [24].…”

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“…Trichoderma sp. is a free-living fungi commonly found in soil and root system and it is known to dissolve phosphate and micro nutrients (Rudresh et al, 2005;Saravanakumar et al, 2013). Interaction Trichoderma sp.…”