Tributyltin Chloride (TBTCl)-Enhanced Exopolysaccharide and Siderophore Production in an Estuarine Alcaligenes faecalis Strain

Khanolkar, Dnyanada S.; Dubey, Santosh Kumar; Naik, Milind Mohan

doi:10.1007/s00244-014-0120-9

Cited by 7 publications

(2 citation statements)

References 38 publications

(61 reference statements)

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“…Within the transport and binding category, a membrane protein (IPR000531) involved in iron uptake by active transport ( Moeck and Coulton, 1998 ) was found over-expressed. Previously, other researchers ( Inoue et al., 2003; Khanolkar et al., 2015 ) suggested that siderophore-like proteins might capture TBT. Thus, the results here reported support this hypothesis.…”

Section: Resultsmentioning

confidence: 99%

Transcriptomes analysis of Aeromonas molluscorum Av27 cells exposed to tributyltin (TBT): Unravelling the effects from the molecular level to the organism

Cruz

Rodrigues

Pinheiro

et al. 2015

Marine Environmental Research

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Aeromonas molluscorum Av27 cells were exposed to 0, 5 and 50 μM of TBT and the respective transcriptomes were obtained by pyrosequencing. Gene Ontology revealed that exposure to 5 μM TBT results in a higher number of repressed genes in contrast with 50 μM of TBT, where the number of over-expressed genes is greater. At both TBT concentrations, higher variations in gene expression were found in the functional categories associated with enzymatic activities, transport/binding and oxidation-reduction. A number of proteins are affected by TBT, such as the acriflavin resistance protein, several transcription-related proteins, several Hsps, ABC transporters, CorA and ZntB and other outer membrane efflux proteins, all of these involved in cellular metabolic processes, important to maintain overall cell viability.Using the STRING tool, several proteins with unknown function were related with others involved in degradation processes, such as the pyoverdine chromophore biosynthetic protein, that has been described as playing a role in the Sn–C cleavage of organotins.This approach has allowed a better understanding of the molecular effects of exposure of bacterial cells to TBT. Furthermore it contributes to the knowledge of the functional genomic aspects of bacteria exposed to this pollutant. Furthermore, the transcriptomic data gathered, and now publically available, constitute a valuable resource for comparative genome analysis.

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Section: Resultsmentioning

confidence: 99%

Transcriptomes analysis of Aeromonas molluscorum Av27 cells exposed to tributyltin (TBT): Unravelling the effects from the molecular level to the organism

Cruz

Rodrigues

Pinheiro

et al. 2015

Marine Environmental Research

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“…The possibility that TBT might directly affect microbial physiology is supported by studies in bacteria, fungi, and other environmental microbial communities (41)(42)(43)(44)(45)(46)(47)(48)(49). Variable levels of microbial susceptibility to TBT have been linked to differences in TBT adsorption, bioaccumulation, enzymatic transformation, efflux and other adaptations (47,(50)(51)(52)(53)(54)(55)(56)(57)(58). It is therefore possible that TBT could also directly impact the composition and activity of host-associated microbial communities.…”

Section: Introductionmentioning

confidence: 99%

Tributyltin exposure leads to increased adiposity and reduced abundance of leptogenic bacteria in the zebrafish intestine

Mueller

et al. 2021

Preprint

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The chemical obesogen tributyltin (TBT) is known to promote fat storage in adipose tissue through direct action on vertebrate cells. TBT also has direct toxic effects on microorganisms, raising the possibility that TBT may also promote fat storage in vertebrates by altering their microbiota. Here we show that exposure of conventionally-reared post-embryonic zebrafish to TBT results in increased adiposity, reduced body size, and altered intestinal microbiota composition including reduced relative abundance of Plesiomonas bacteria. To test if those microbiota alterations affected host adiposity, we exposed conventionally-reared zebrafish to intestinal bacterial strains representative of TBT-altered taxa. We found that introduction of a Plesiomonas strain into conventionally-reared zebrafish was sufficient to reduce adiposity and alter intestinal microbiota composition. Using new long-term gnotobiotic zebrafish husbandry methods, we found that colonization of germ-free zebrafish with Plesiomonas was sufficient to reduce host adiposity. Together these results show the leptogenic activity of Plesiomonas on zebrafish hosts, indicating that the ability of TBT to increase adiposity in vivo may be due in part to TBT-mediated modification of the abundance of leptogenic bacteria like Plesiomonas. These findings underscore how complex reciprocal interactions between animals and their microbial and chemical environments can influence energy balance and metabolic health.IMPORTANCEObesogens are environmental chemicals that promote fat storage and are generally thought to exert this effect directly on animal cells. Using zebrafish, we show that the obesogen tributyltin can also promote fat storage by acting upon intestinal microbiota via reduction of bacteria that are sufficient to reduce fat storage.

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Microbial Remediation of Organometals and Oil Hydrocarbons in the Marine Environment

Cruz

Cavaleiro

Paulo

et al. 2016

Marine Pollution and Microbial Remediation

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Tributyltin Chloride (TBTCl)-Enhanced Exopolysaccharide and Siderophore Production in an Estuarine Alcaligenes faecalis Strain

Cited by 7 publications

References 38 publications

Transcriptomes analysis of Aeromonas molluscorum Av27 cells exposed to tributyltin (TBT): Unravelling the effects from the molecular level to the organism

Transcriptomes analysis of Aeromonas molluscorum Av27 cells exposed to tributyltin (TBT): Unravelling the effects from the molecular level to the organism

Tributyltin exposure leads to increased adiposity and reduced abundance of leptogenic bacteria in the zebrafish intestine

Microbial Remediation of Organometals and Oil Hydrocarbons in the Marine Environment

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