Transcriptomes analysis of Aeromonas molluscorum Av27 cells exposed to tributyltin (TBT): Unravelling the effects from the molecular level to the organism

Abstract

Aeromonas molluscorum Av27 cells were exposed to 0, 5 and 50 μM of TBT and the respective transcriptomes were obtained by pyrosequencing. Gene Ontology revealed that exposure to 5 μM TBT results in a higher number of repressed genes in contrast with 50 μM of TBT, where the number of over-expressed genes is greater. At both TBT concentrations, higher variations in gene expression were found in the functional categories associated with enzymatic activities, transport/binding and oxidation-reduction. A number of proteins are affected by TBT, such as the acriflavin resistance protein, several transcription-related proteins, several Hsps, ABC transporters, CorA and ZntB and other outer membrane efflux proteins, all of these involved in cellular metabolic processes, important to maintain overall cell viability.Using the STRING tool, several proteins with unknown function were related with others involved in degradation processes, such as the pyoverdine chromophore biosynthetic protein, that has been described as playing a role in the Sn–C cleavage of organotins.This approach has allowed a better understanding of the molecular effects of exposure of bacterial cells to TBT. Furthermore it contributes to the knowledge of the functional genomic aspects of bacteria exposed to this pollutant. Furthermore, the transcriptomic data gathered, and now publically available, constitute a valuable resource for comparative genome analysis.

Tributyltin exposure leads to increased adiposity and reduced abundance of leptogenic bacteria in the zebrafish intestine

“…The possibility that TBT might directly affect microbial physiology is supported by studies in bacteria, fungi, and other environmental microbial communities (41)(42)(43)(44)(45)(46)(47)(48)(49). Variable levels of microbial susceptibility to TBT have been linked to differences in TBT adsorption, bioaccumulation, enzymatic transformation, efflux and other adaptations (47,(50)(51)(52)(53)(54)(55)(56)(57)(58). It is therefore possible that TBT could also directly impact the composition and activity of host-associated microbial communities.…”

Transcriptomic effects of tributyltin (TBT) in zebrafish eleutheroembryos. A functional benchmark dose analysis

“…It has been proposed that the mode of action (MoA) of TBT is via covalent binding with retinoid X receptors (RXR) (le Maire et al, 2009). Although its effects at phenotypical, organismal and transcriptomic levels have been explained in other species (Baker and J o u r n a l P r e -p r o o f Hardiman, 2014;Cruz et al, 2015b;Zhang et al, 2018), not much is known on its transcriptomic effects in zebrafish eleutheroembryos. This fish species is considered an excellent vertebrate model for human and environmental toxicology, including endocrine disruption, thanks to its easy rearing and to the characteristics of their freeswimming late embryonic stages (Kimmel et al, 1995a;Parichy et al, 2009;Raldúa and Piña, 2014;Scholz and Mayer, 2008;Segner, 2009;Stegeman et al, 2010;Strähle et al, 2012).…”

Análise das bases moleculares da tolerância ao íon cloreto em bactérias acidófilas utilizadas em biolixiviação

“…As proteínas codificadas por estes genes desempenham papéis importantes na resposta das células de A. ferrooxidans ao NaCl. Este tipo de validação tem sido utilizado em vários trabalhos (Yoder-Himes et al, 2009;Croucher & Thomson, 2010;Fu et al, 2014;Cruz et al, 2015). Os resultados são mostrados na Tabela 3.3 e na Figura 3.5.…”