Triazenos e atividade antibacteriana

Hörner, Manfredo; Giglio, Vinícius Feltrin; Santos, Aline Joana Rolina Wohlmuth Alves dos; Westphalen, André Bilibio; Iglesias, Bernardo A.; Martins, Paulo Roberto; Amaral, Carlos Henrique do; Michelot, Tiago Mozaquatro; Reetz, Luiz Gustavo Brenner; Bertoncheli, Claudia de Mello; Paraginski, Gustavo Luiz; Hörner, Rosmari

doi:10.1590/s1516-93322008000300014

Cited by 7 publications

(4 citation statements)

References 24 publications

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“…The experiments were performed in triplicate and the plates were incubated at 28 • C for 14 days. The MIC was determined as the lowest concentration of the extract capable of completely inhibiting pathogen growth (Hörner et al, 2008).…”

Section: Determination Of the Minimum Inhibitory Concentration For Pathogenic Fungimentioning

confidence: 99%

Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

et al. 2021

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Plant diseases caused by phytopathogens are responsible for significant crop losses worldwide. Resistance induction and biological control have been exploited in agriculture due to their enormous potential. In this study, we investigated the antimicrobial potential of endophytic fungi of leaves and petioles of medicinal plants Vochysia divergens and Stryphnodendron adstringens located in two regions of high diversity in Brazil, Pantanal, and Cerrado, respectively. We recovered 1,304 fungal isolates and based on the characteristics of the culture, were assigned to 159 phenotypes. One isolate was selected as representative of each phenotype and studied for antimicrobial activity against phytopathogens. Isolates with better biological activities were identified based on DNA sequences and phylogenetic analyzes. Among the 159 representative isolates, extracts from 12 endophytes that inhibited the mycelial growth (IG) of Colletotrichum abscissum (≥40%) were selected to expand the antimicrobial analysis. The minimum inhibitory concentrations (MIC) of the extracts were determined against citrus pathogens, C. abscissum, Phyllosticta citricarpa and Xanthomonas citri subsp. citri and the maize pathogen Fusarium graminearum. The highest activity against C. abscissum were from extracts of Pseudofusicoccum stromaticum CMRP4328 (IG: 83% and MIC: 40 μg/mL) and Diaporthe vochysiae CMRP4322 (IG: 75% and MIC: 1 μg/mL), both extracts also inhibited the development of post-bloom fruit drop symptoms in citrus flowers. The extracts were promising in inhibiting the mycelial growth of P. citricarpa and reducing the production of pycnidia in citrus leaves. Among the isolates that showed activity, the genus Diaporthe was the most common, including the new species D. cerradensis described in this study. In addition, high performance liquid chromatography, UV detection, and mass spectrometry and thin layer chromatography analyzes of extracts produced by endophytes that showed high activity, indicated D. vochysiae CMRP4322 and P. stromaticum CMRP4328 as promising strains that produce new bioactive natural products. We report here the capacity of endophytic fungi of medicinal plants to produce secondary metabolites with biological activities against phytopathogenic fungi and bacteria. The description of the new species D. cerradensis, reinforces the ability of medicinal plants found in Brazil to host a diverse group of fungi with biotechnological potential.

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Section: Determination Of the Minimum Inhibitory Concentration For Pathogenic Fungimentioning

confidence: 99%

Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

et al. 2021

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“…Hörner et al . 37 evaluated the activity of 15 triazenide compounds including salts of vanadium and potassium hydroxytriazene complexes against various bacteria involving extended-spectrum β-lactamase (ESBL) strains. The compounds were active against multidrug-resistant bacteria including ESBL-, metallo-β-lactamases (MBL)-, and adenosine 3,5-cyclic monophosphate (AmpC) gene-producing strains.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the biological potential of diaryltriazene-derived triazene compounds

Figueiredo

Filho

Sodré

et al. 2021

Sci Rep

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In the present study, novel, 1,3-diaryltriazene-derived triazene compounds were synthesized and tested. Triazenes are versatile and belong to a group of alkylating agents with interesting physicochemical properties and proven biological activities. This study describes the synthesis, molecular and crystalline structure, biological activity evaluation, and antifungal and antimicrobial potentials of 1,3-bis(X-methoxy-Y-nitrophenyl)triazenes [X = 2 and 5; Y = 4 and 5]. The antimicrobial and antifungal activities of the compounds were tested by evaluating the sensitivity of bacteria (American Type Culture Collection, ATCC) and clinical isolates to their solutions using standardized microbiological assays, cytotoxicity evaluation, and ecotoxicity tests. The antimicrobial potentials of triazenes were determined according to their minimum inhibitory concentrations (MICs); these compounds were active against gram-positive and gram-negative bacteria, with low MIC values. The most surprising result was obtained for T3 having the effective MIC of 9.937 µg/mL and antifungal activity against Candida albicans ATCC 90028, C. parapsilosis ATCC 22019, and C. tropicallis IC. To the best of our knowledge, this study is the first to report promising activities of triazene compounds against yeast and filamentous fungi. The results showed the potential utility of triazenes as agents affecting selected resistant bacterial and fungal strains.

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“…The sample was tested in triplicate on each microplate. The MIC was considered as the lowest concentration of the sample or controls capable of inhibiting the growth of 90% of the strains (Hörner et al, 2008). The microplates were incubated in an oven at 37ºC for 24 h. Resazurin developer (100 µg/mL) was added to each well of the microplates with the bacteria (30 µL).…”

Section: Minimum Inhibitory Concentration (Mic) Testmentioning

confidence: 99%

Chemical characterization, antimicrobial activity and cardiac effects in rat heart of the ethyl acetate fraction of Syzygium cumini (L.) Skeels

Mota

Santos

et al. 2023

RSD

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Plants are important sources of secondary metabolites responsible for various biological activities. In this research, was evaluated the effect action ethyl acetate fraction of Syzygium cumini leaves (Sc-AcOEt) in the antimicrobial activity, contractile and electrical effects in isolated rat hearts and the chemical characterization. Antimicrobial activity was assessed using the disk-diffusion method and the determination of the minimum inhibitory concentration. The contractile and electrical effects were tested using the Langendorf method. The chemical characterization was evaluated through quantification and HPLC analyzes. P. aeruginosa was not viable in any concentration of the fraction (MIC 0,125 mg/mL), while S. aureus showed a MIC of 0.5 mg/mL. K. pneumoniae and E. coli had the highest MIC (1 mg/mL). A reduction in LVDP was observed after heart perfusion with 0.1 mg/mL of Sc-AcOEt. No change was observed in the duration of systole and diastole at all concentrations and in the electrocardiographic parameters. Total phenols, total flavonoids and total flavonols contents of Sc-AcOEt, calculated from the respective calibration curves, were 21.556µg/mg AGE, 617.222µg/mg QE and 315,222µg/mg RE, respectively. In addition, the HPLC analysis of Sc-AcOEt showed the presence of gallic acid, quercetin-3-glucoside, naringin and myricetin as main components. Thus, the Sc-AcOEt has important antimicrobial and cardiac activities possibly due to its chemical constituents.

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Triazenos e atividade antibacteriana

Abstract: Estes resultados demonstraram a potencial atividade biológica destes compostos contra bactérias Gram-positivas e Gram-negativas.

Cited by 7 publications

References 24 publications

Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

Endophytes of Brazilian Medicinal Plants With Activity Against Phytopathogens

Assessment of the biological potential of diaryltriazene-derived triazene compounds

Chemical characterization, antimicrobial activity and cardiac effects in rat heart of the ethyl acetate fraction of Syzygium cumini (L.) Skeels

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