Triacylglycerol regioisomers in human milk resolved with an algorithmic novel electrospray ionization tandem mass spectrometry method

Kallio, Heikki; Nylund, Matts; Boström, Pontus; Yang, Baoru

doi:10.1016/j.foodchem.2017.04.122

Cited by 87 publications

(76 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These FAs combinations in the TAGs detected by DI-ESI-MS are in accordance with the literature, and it represents more than half of all FAs in the samples evaluated in the present study. The results obtained agreed with a study by Kallio et al, 37 in which they evaluated TAGs in pooled Chinese and Finnish HM samples, it was concluded that these FAs may be influenced by factors such as local eating habits, but remain the predominant FAs.…”

Section: Di-esi(+)-mssupporting

confidence: 90%

Lipid Profile Determination by Direct Infusion ESI-MS and Fatty Acid Composition by GC-FID in Human Milk Pools by Folch and Creamatocrit Methods

Rydlewski¹,

Silva²,

Manin³

et al. 2019

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Human milk (HM) is extremely important to the baby, containing a varied and balanced range of nutrients fundamental to the ideal development of the newborn. Among these nutrients, HM lipids provide 40-60% of the energy. In HM banks, the creamatocrit method is employed as a quick and solvent-free tool, however, since Folch methodology is considered reference for lipid extraction, this study compared the fatty acid (FA) composition and the lipid profile by gas chromatography with flame ionization detector (GC-FID) and direct infusion by electrospray ionization mass spectrometry (DI-ESI-MS) in pools of different HM phases, obtained from Folch and creamatocrit methods, in order to elucidate which method provides better information about HM lipids. Lipid compositon results by Folch were superior to the results obtained by creamatocrit in HM pools investigated. It means, the lipid profiles of all HM pools were more intense, and the number of FA identified by Folch was also higher in comparison to the creamatocrit method (21 and 6 FAs, respectively). GC-FID analysis in combination with principal component analysis (PCA) divided the methods, confirming a greater contribution of the Folch method. Therefore, Folch methodology revealed an effective lipid extraction in comparison to creamatocrit method.

show abstract

Section: Di-esi(+)-mssupporting

confidence: 90%

Lipid Profile Determination by Direct Infusion ESI-MS and Fatty Acid Composition by GC-FID in Human Milk Pools by Folch and Creamatocrit Methods

Rydlewski¹,

Silva²,

Manin³

et al. 2019

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

show abstract

“…These results interpreted shows that the preferred FA species of sn‐glycerol‐3‐phosphate acyltransferase and 1‐acyl‐sn‐glycerol‐3‐phosphate acyltransferase in marine mammals are different to those in fish and other animals, or that diacylglycerol acyltransferase in marine mammals favors 1,2‐dipalmitoyl‐sn‐glycerol formed from 1,2‐dipalmitoyl‐sn‐glycerol‐3‐phosphatidate if HUFA is the reaction substrate. Heikki Kallio and coworkers revealed hundreds of TAGs regioisomers in human milk samples by UPLC resolution of lithiated TAGs followed by daughter scan of positive ions for quantitative analysis with ESI‐MS/MS. The research discovered that when present in the same TAG molecule together with C18 FAs, palmitic acid was typically in the sn‐2 position.…”

Section: Applications In Foodmentioning

confidence: 99%

Research advances based on mass spectrometry for profiling of triacylglycerols in oils and fats and their applications

Wei

Xie

et al. 2018

Electrophoresis

View full text Add to dashboard Cite

Vegetable oils and animal fats are dietary source of lipids that play critical and multiple roles in biological function. Triacylglycerols (TAGs) are the principal component of oils and fats with significant difference in profile among different oils and fats. TAG profiling is essential for nutritional evaluation, quality control and assurance of safety in oils and fats. However, analysis of TAGs is a challenging task because of the complicated composition of TAGs and their similar physicochemical properties in oils and fats. The rapid development of mass spectrometry (MS) technology in recent years makes it possible to analyze the composition, content and structure of TAGs in the study of the physical, chemical and nutritional properties of oils, fats and related products. This review described the research advancement based on MS for profiling of TAGs in oil, fat and their applications in food. The application of MS, including direct infusion strategies, and its combination with chromatography, gas chromatography-MS (GC-MS) and liquid chromatography-MS (LC-MS), in the analysis of TAGs were reviewed. The advantages and disadvantages of these analytical methods with relevant applications for TAGs analysis in food were also described.

show abstract

“…Desta forma, é possível coletar os lipídios extraídos de maneira eficaz e dosá-los utilizando métodos gravimétricos. 37,38 Nestes métodos, é realizada a evaporação total do solvente e a dosagem lipídica é quantificada pela diferença do peso da vidraria vazia ao se iniciar a análise e vidraria com a fase lipídica, após a extração lipídica. 39 Dentre as metodologias amplamente empregadas para a extração lipídica, destacase o procedimento proposto por Soxhlet (1879), que utiliza a extração a quente com o refluxo de solvente puro, que pode ser éter de petróleo, éter dietílico ou n-hexano.…”

Section: Extração E Posterior Dosagem Lipídica Do Leite Humanounclassified

“…36,47,48 Kallio et al (2017) utilizaram a SPE para isolar TAGs e Pérez-Cejuela et al (2018) para extrair fosfolipídios, utilizarando cartuchos à base de sílica gel e os resultados de ambos os trabalhos mostraram-se eficientes para tais objetivos. 36,37 Já Garwolińska et al (2017) empregaram a técnica de micro-extração em fase sólida (SPME) para a extração de lipídios, com o objetivo de identificar glicerofosfolipídios, glicerolipídios e esfingolipídios. Os autores concluíram que este método demonstrou ser rápido e simples, além de permitir a extração de uma ampla faixa de lipídios de LH, sem o uso de solventes tóxicos, como o clorofórmio.…”

Section: Extração E Posterior Dosagem Lipídica Do Leite Humanounclassified

“…Além disso, os pares de TAGs regioisoméricos das mesmas combinações de AGs tiveram retenção idêntica e não se separaram um do outro. 37 A infusão direta por ESI-MS é uma técnica rápida, precisa e simples que pode ser utilizada para a análise lipídica de possíveis TAGs do LH. 74,75 Apesar destas vantagens, há a possibilidade de ocorrerem alguns problemas associados à técnica, como por exemplo: supressão de íons, interferência isotópica e diferenciação de isômeros.…”

Section: Espectrometria De Massas (Ms)unclassified

See 1 more Smart Citation

Analytical Methods Used for the Determination of Lipids in Human Milk: A Review

Rydlewski¹,

Pizzo²,

Manin³

et al. 2020

Rev. Virtual Quim.

View full text Add to dashboard Cite

Human milk is considered the ideal and most complete food for the neonate, due to its balanced nutritional composition, which contributes to the full physical and neurological development of the infant. The lipids present in human milk are sources of saturated, monounsaturated and polyunsaturated fatty acids. The polyunsaturated fatty acids of the omega-6 series, such as linoleic acid and omega-3 series, such as α-linolenic acid, are essential and are also precursors of long-chain polyunsaturated fatty acids, such as arachidonic acid, eicosapentaenoic acid and docosahexaenoic acid, which are involved in a number of functions, such as the development of the nervous system during childhood and the increase in the intelligence quotient of the infant. Due to the lipid importance of human milk, the aim of this review article was to present the analytical methods that are currently being employed to determine its lipid composition. There are few scientific advances in lipid extraction methods and to date, most studies have evaluated the composition of fatty acids by gas chromatography. Liquid chromatography and mass spectrometry have been widely used to identify and quantify different classes of lipids of human milk. O leite humano é considerado o alimento ideal e mais completo para o neonato, devido à sua composição nutricional equilibrada, contribuindo para o pleno desenvolvimento físico e neurológico do lactente. Os lipídios presentes no leite humano são fontes de ácidos graxos saturados, monoinsaturados e poliinsaturados. Os ácidos graxos poli-insaturados da série ômega-6, como o ácido linoleico e da série ômega-3, como o α-linolênico, são essenciais e também são precursores de ácidos graxos poli-insaturados de cadeia longa, como o ácido araquidônico, ácido eicosapentaenoico e ácido docosahexaenoico, que estão envolvidos no desenvolvimento do sistema nervoso e no aumento do quociente de inteligência do lactente. Devido à importância lipídica do leite humano, o objetivo deste artigo de revisão foi apresentar os métodos analíticos que têm sido atualmente mais empregados para determinar sua composição lipídica. Há poucos avanços científicos quanto aos métodos de extração lipídica e até o presente momento, grande parte dos estudos avaliaram, a composição em ácidos graxos, a partir de cromatografia em fase gasosa. A cromatografia líquida e a espectrometria de massas têm sido utilizadas para identificação e quantificação de distintas classes lipídicas presentes no leite humano.

show abstract

Triacylglycerol regioisomers in human milk resolved with an algorithmic novel electrospray ionization tandem mass spectrometry method

Cited by 87 publications

References 38 publications

Lipid Profile Determination by Direct Infusion ESI-MS and Fatty Acid Composition by GC-FID in Human Milk Pools by Folch and Creamatocrit Methods

Lipid Profile Determination by Direct Infusion ESI-MS and Fatty Acid Composition by GC-FID in Human Milk Pools by Folch and Creamatocrit Methods

Research advances based on mass spectrometry for profiling of triacylglycerols in oils and fats and their applications

Analytical Methods Used for the Determination of Lipids in Human Milk: A Review

Contact Info

Product

Resources

About