Treponema denticola (ex Brumpt 1925) sp. nov., nom. rev., and Identification of New Spirochete Isolates from Periodontal Pockets

Chan, E. C. S.; Siboo, R.; Keng, Teresa; Psarra, N.; Hurley, Rosalinde; Cheng, S.-L.; Iugovaz, Irene

doi:10.1099/00207713-43-2-196

Cited by 85 publications

(58 citation statements)

References 9 publications

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“…In T. denticola, the flagellar filaments are attached at one end of the cell, the other being free in the periplasm; the flagellar filaments originating at opposite ends of the same cell overlap at mid-cell (Chan et al, 1993). The formation and rotation of the flagellar filament is associated with its attachment to the flagellar basal body, consisting of the motor, regulator, and export machinery, which is embedded in the cytoplasmic membrane, and protrudes from the cell end (Limberger, 2004).…”

Section: Flagellar Basal Body Organizationmentioning

confidence: 99%

Native cellular architecture of Treponema denticola revealed by cryo-electron tomography

Izard¹,

Hsieh²,

Limberger³

et al. 2008

Journal of Structural Biology

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Using cryo-electron tomography, we are developing a refined description of native cellular structures in the pathogenic spirochete Treponema denticola. Tightly organized bundles of periplasmic flagella were readily observed in intact plunge-frozen cells. The periplasmic space was measured in both wild-type and aflagellate strains, and found to widen by less than the diameter of flagella when the latter are present. This suggests that a structural change occurs in the peptidoglycan layer to accommodate the presence of the flagella. In dividing cells, the flagellar filaments were found to bridge the cytoplasmic cylinder constriction site. Cytoplasmic filaments, adjacent to the inner membrane, run parallel to the tightly organized flagellar filaments. The cytoplasmic filaments may be anchored by a narrow plate-like structure. The tapering of the cell ends was conserved between cells, with a patella-shaped structure observed in the periplasm at the tip of each cytoplasmic cylinder. Several incompletely characterized structures have been observed in the periplasm between dividing cells, including a cable-like structure linking two cytoplasmic cylinders and complex foil-shaped structures.

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Section: Flagellar Basal Body Organizationmentioning

confidence: 99%

Native cellular architecture of Treponema denticola revealed by cryo-electron tomography

Izard¹,

Hsieh²,

Limberger³

et al. 2008

Journal of Structural Biology

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“…denticola strain 35405 was initially isolated and designated as the type strain by Chan et al (7). Bacteria used in this study were obtained from the American Type Culture Collection.…”

Section: Bacteriamentioning

confidence: 99%

Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes

Seshadri

Myers

Tettelin

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

250

314

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We present the complete 2,843,201-bp genome sequence of Treponema denticola (ATCC 35405) an oral spirochete associated with periodontal disease. Analysis of the T. denticola genome reveals factors mediating coaggregation, cell signaling, stress protection, and other competitive and cooperative measures, consistent with its pathogenic nature and lifestyle within the mixed-species environment of subgingival dental plaque. Comparisons with previously sequenced spirochete genomes revealed specific factors contributing to differences and similarities in spirochete physiology as well as pathogenic potential. The T. denticola genome is considerably larger in size than the genome of the related syphiliscausing spirochete Treponema pallidum. The differences in gene content appear to be attributable to a combination of three phenomena: genome reduction, lineage-specific expansions, and horizontal gene transfer. Genes lost due to reductive evolution appear to be largely involved in metabolism and transport, whereas some of the genes that have arisen due to lineage-specific expansions are implicated in various pathogenic interactions, and genes acquired via horizontal gene transfer are largely phagerelated or of unknown function.

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“…It is also apparent that different filament arrangements have been observed within the same treponeme strain. Chan et al [51] described 7: denticola strain ATCC 35405 as having 10 axial filaments -and therefore a 5-10-5 endoflagella arrangement -whereas, in previous studies, I: denticola strains have been documented as having 2-4-2, 3-6-3 and 5-10-5 arrangements [16,53,601.…”

Section: Structurementioning

confidence: 99%

“…7: socranskii has similar requirements [19]. However, Chan et al [51] described 7: denticola as being primarily an aminoacid fermenter that does not use the glycolytic pathway as a major energy source. This study found that only sodium bicarbonate was essential for growth of the six treponeme isolates investigated.…”

Section: Mtygvsmentioning

confidence: 99%

See 1 more Smart Citation

The challenge of growing oral spirochaetes

Wardle

1997

Journal of Medical Microbiology

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Treponema denticola (ex Brumpt 1925) sp. nov., nom. rev., and Identification of New Spirochete Isolates from Periodontal Pockets

Cited by 85 publications

References 9 publications

Native cellular architecture of Treponema denticola revealed by cryo-electron tomography

Native cellular architecture of Treponema denticola revealed by cryo-electron tomography

Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes

The challenge of growing oral spirochaetes

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