The challenge of growing oral spirochaetes

Wardle, Helen M.

doi:10.1099/00222615-46-2-104

Cited by 9 publications

(5 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…needs and extremely low oxygen tolerance (2,11,12). Despite the progress and development of new culture media and techniques in clinical microbiology, Treponema species remain excluded from many culture-dependent studies (13).…”

mentioning

confidence: 99%

Passive Filtration, Rapid Scanning Electron Microscopy, and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Treponema Culture and Identification from the Oral Cavity

et al. 2019

View full text Add to dashboard Cite

We present here a new passive-filtration-based culture device combined with rapid identification with a new electron microscope (Hitachi TM4000) for the detection and culture of Treponema species from the human oral cavity. Of the 44 oral samples cultivated, 15 (34%) were found to be positive for Treponema using electron microscopy and were also culture positive. All were subcultured on agar plates; based on genome sequencing and analyses, 10 were strains of Treponema pectinovorum and 5 were strains of Treponema denticola. The 29 samples that were negative for Treponema remained culture negative. In addition, 14 Treponema species ordered from the DSMZ collection were cultured in the T-Raoult culture medium optimized here. Finally, matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) was used and 30 novel spectra were added to the MALDI-TOF MS database. We have successfully developed a new and effective method for treponemal detection, culture, and identification.

show abstract

mentioning

confidence: 99%

Passive Filtration, Rapid Scanning Electron Microscopy, and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Treponema Culture and Identification from the Oral Cavity

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Many studies have employed different collection techniques, including paper points, sterile cotton swaps, curettes or strips, in the sulcus, mesial and distal surfaces of teeth and were mainly focused on the subgingival plaque ( Teles et al, 2007 ; Wang et al, 2013 ). The methods used to sample supragingival or subgingival plaque were described by Wardle (1997) . Studies have been performed to ascertain the method of sampling that will yield the most organisms ( Renvert et al, 1992 ) and to determine the area from which it is appropriate to take the sample ( Katsanoulas et al, 1992 ).…”

Section: Discussionmentioning

confidence: 99%

Quantitative Molecular Detection of 19 Major Pathogens in the Interdental Biofilm of Periodontally Healthy Young Adults

et al. 2016

View full text Add to dashboard Cite

In oral health, the interdental spaces are a real ecological niche for which the body has few or no alternative defenses and where the traditional daily methods for control by disrupting biofilm are not adequate. The interdental spaces are the source of many hypotheses regarding their potential associations with and/or causes of cardiovascular disease, diabetes, chronic kidney disease, degenerative disease, and depression. This PCR study is the first to describe the interdental microbiota in healthy adults aged 18–35 years-old with reference to the Socransky complexes. The complexes tended to reflect microbial succession events in developing dental biofilms. Early colonizers included members of the yellow, green, and purple complexes. The orange complex bacteria generally appear after the early colonizers and include many putative periodontal pathogens, such as Fusobacterium nucleatum. The red complex (Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola) was considered the climax community and is on the list of putative periodontal pathogens. The 19 major periodontal pathogens tested were expressed at various levels. F. nucleatum was the most abundant species, and the least abundant were Actinomyces viscosus, P. gingivalis, and Aggregatibacter actinomycetemcomitans. The genome counts for Eikenella corrodens, Campylobacter concisus, Campylobacter rectus, T. denticola, and Tannerella forsythensis increased significantly with subject age. The study highlights the observation that bacteria from the yellow complex (Streptococcus spp., S. mitis), the green complex (E. corrodens, Campylobacter gracilis, Capnocytophaga ochracea, Capnocytophaga sputigena, A. actinomycetemcomitans), the purple complex (Veillonella parvula, Actinomyces odontolyticus) and the blue complex (A. viscosus) are correlated. Concerning the orange complex, F. nucleatum is the most abundant species in interdental biofilm. The red complex, which is recognized as the most important pathogen in adult periodontal disease, represents 8.08% of the 19 bacteria analyzed. P. gingivalis was detected in 19% of healthy subjects and represents 0.02% of the interdental biofilm. T. forsythensis and T. denticola (0.02 and 0.04% of the interdental biofilm) were detected in 93 and 49% of healthy subjects, respectively. The effective presence of periodontal pathogens is a strong indicator of the need to develop new methods for disrupting interdental biofilm in daily oral hygiene.

show abstract

“…Therefore, we could not identify bacterial components and bacteria that were already eliminated by the immune system of the healthy subjects, bacteria that transformed into a non‐cultivable state despite remaining vital, or bacteria that did not survive until being processed. In addition, the analysis is highly technique‐sensitive, as some bacteria might not survive the culturing process or at least would not form colonies (Wardle ). With highly specific tests based on genetic analysis methods (FISH etc.…”

Section: Discussionmentioning

confidence: 99%

Effect of application of a PVP‐iodine solution before and during subgingival ultrasonic instrumentation on post‐treatment bacteraemia: a randomized single‐centre placebo‐controlled clinical trial

Sahrmann

Manz

Attin

et al. 2015

J Clinic Periodontology

View full text Add to dashboard Cite

Effect of application of a PVPiodine solution before and during subgingival ultrasonic instrumentation on posttreatment bacteraemia: a randomized single-centre placebo-controlled clinical trial Abstract Background: To assess the effect of concomitant subgingival rinsing with 10% PVP-iodine during subgingival instrumentation on the prevalence and magnitude of bacteraemia of oral origin. Materials and Methods: Subgingival instrumentation was performed with water or PVP-iodine rinse in patients with periodontitis. Prior to instrumentation, subjects gargled for 1 min with the allocated liquid. Pockets were then rinsed for 1 min and subgingivally instrumented with liquid-cooled (water/PVP-iodine) ultrasonic scalers (1 min). Two minutes later, a blood sample from the arm vein was drawn using a lysis centrifugation blood culture system for quantitative microbiological analysis. Non-parametric statistical tests were performed to assess differences in the prevalence and extent of bacteraemia between groups. Results: Of the 19 samples in each group, oral-borne bacteraemia was detected in 10 of the control and 2 of the test samples. With an average of 3.0 [1; 5] colony forming units, significantly less bacteria and bacteraemia were found in the test group compared to the controls (12.2 [1; 46]) (p = 0.003). Anaerobic bacteria were not found in the test group. Conclusions: Bacteraemia after subgingival instrumentation with concomitant PVP-iodine rinsing is reduced but not eliminated. Therefore, it might be recommended for patients at a high risk of endocarditis or infection of endoprostheses. However, preventive antibiotic treatment should not be omitted.

show abstract

The challenge of growing oral spirochaetes

Cited by 9 publications

References 46 publications

Passive Filtration, Rapid Scanning Electron Microscopy, and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Treponema Culture and Identification from the Oral Cavity

Passive Filtration, Rapid Scanning Electron Microscopy, and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Treponema Culture and Identification from the Oral Cavity

Quantitative Molecular Detection of 19 Major Pathogens in the Interdental Biofilm of Periodontally Healthy Young Adults

Effect of application of a PVP‐iodine solution before and during subgingival ultrasonic instrumentation on post‐treatment bacteraemia: a randomized single‐centre placebo‐controlled clinical trial

Contact Info

Product

Resources

About