Treponema brennaborense sp. nov., a novel spirochaete isolated from a dairy cow suffering from digital dermatitis

Abstract

A novel Treponema species was isolated from an ulcerative lesion of a cow suffering from digital dermatitis (DD), a disease which causes painful ulcerations along the coronary band. Among other anaerobic bacteria, high numbers of spirochaetes have been regularly found in DD lesions. Here data are presented of a spirochaete isolated from a DD ulcer. By chemotaxonomy, protein analysis and comparative 165 rDNA sequence analysis this isolate was classified as a treponeme that differed from all Treponema species described previously. The only isolate, DD5/3l, for which the name Treponema brennaborense is proposed, is designated the type strain of the novel species.The strain is a small, highly motile spirochaete that has two periplasmic flagella, one flagellum being attached at each cell pole. Strain DD5/3f exhibits a-glucosidase and N-acetyl-p-glucosaminidase activity and growth is inhibited by rabbit serum. T. brennaborense was phylogenetically most closely related (89.5 O/ O 165 rRNA similarity) to Treponema maltophilum, an oral spirochaete isolated from a periodontitis patient.

Exilispira thermophila gen. nov., sp. nov., an anaerobic, thermophilic spirochaete isolated from a deep-sea hydrothermal vent chimney

“…1a) has been reported for other members of the genera Spirochaeta and Treponema (e.g. Fracek & Stolz, 1985;Schrank et al, 1999;Zhilina et al, 1996). Moreover, the cells of strain RASEN T had hook-shaped ends.…”

Acetogenesis from H2plus CO2and nitrogen fixation by an endosymbiotic spirochete of a termite-gut cellulolytic protist

“…2) revealed that the sequences of the endosymbionts were derived from spirochetes of the genus Treponema and belonged to the previously defined termite Treponema cluster II (26), which consists of the ectosymbionts attached to the cell surface of gut protists as far as their localizations were examined (27,28). This cluster formed a clade with saccharolytic treponemes that contained Treponema brennaborense, an isolate from a digital dermatitis ulcer of a cow (29), as a close cultured relative, but distantly related to the termite Treponema cluster I that comprises isolates and clone sequences from termite guts. We propose a species, "Candidatus Treponema intracellularis" for this previously unidentified endosymbiont (SI Text).…”

Isolation and characterisation of a novel spirochaete from severe virulent ovine foot rot

“…Motile spirochaetes were also observed and successfully cultured [9,10]. Recently, spirochaetes have been cultured successfully from cases of a related disease of cattle, bovine digital dermatitis, in the USA [11], Germany [12] and the UK [13]. The latter study used immunomagnetic separation to isolate a spirochaete from affected cattle [13].…”